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The map label for this gene is rlmN

Identifier: 209694315

GI number: 209694315

Start: 809739

End: 810890

Strand: Direct

Name: rlmN

Synonym: VSAL_I0726

Alternate gene names: 209694315

Gene position: 809739-810890 (Clockwise)

Preceding gene: 209694314

Following gene: 209694316

Centisome position: 24.35

GC content: 40.62

Gene sequence:

>1152_bases
ATGACTACAACTAAAACCAATCTACTGGACTTTGATCGTAAAGGACTTCGAGCATTTTTTTCTGAGGAATTAGGAGAGAA
AGCCTTCCGAGCAGATCAAATCATGAAGTGGATGTATCACTTTGGTTGTGATGACTTCGATCAAATGAACAACATCAACA
AAAAGCTTCGTGAAAAGCTAAAGCAGAAATGTGAAATTCGAGCACCCTACGTATCTGAAGCTCAGCATTCAGTTGATGGC
ACCATCAAATGGGCGATGAAAGTAGGCGATCAAGATGTTGAGACGGTTTATATCCCTGATGGCGATCGCGCAACCTTATG
CGTATCTTCTCAAGTGGGCTGTGCACTTGCTTGTACATTCTGCTCAACAGCACAACAAGGCTTTAACCGTAACCTTAAAG
TTTCTGAAATTGTCGGTCAAATTTGGCGTGCCGCTCGTGAAATTGGTTTAGAAAAAGAAACGGGTCGCCGTCCAATTACG
AATGTCGTAATGATGGGCATGGGTGAACCATTGCTTAATATGAAAAATCTAATGCCATCATTAGATATCATGCTTGATGA
TCTAGGTTTTGGTTTGTCTAAACGTCGTGTCACTGTATCTACATCAGGTGTTGTTTCTGGCTTAGAACAAATGATCGGCA
AAGTTGATGTCGCTTTAGCTATTTCTCTTCATGCACCTACGGATAAATTGCGCAGTGAGATCATGCCAATTAATGATCGT
TGGAATATTGAAGCGTTTTTAGCGTGTGTTCGTGAATACATTGCATCATCAAATGCTAACCGTGGCCGTGTAACGGTAGA
GTATGTACTTTTAGATCATATAAATGACGATATGGATCATGCAAGACAACTCGCAGAATTGCTAAAAGATACCCCGGCAA
AGATTAATTTGATTCCATTTAACCCATATCCTGGGTCACCATACAAAAAGCCAAGTAATTCACGTATCGATCGATTCATG
AAAACATTGATGGAATACGATTTTACGGTAACGGTTCGTAGAACTCGTGGTGATGATATTGATGCCGCTTGTGGTCAATT
GGTCGGTGATGTAATCGATAGAACTAAACGCACTCAAGTTAAACAGCAAGGTGAGGCTATCCCTGTAAAACTGTTTAATC
TTGATGTATTACAGCAAGGGAAAGCAGAATGA

Upstream 100 bases:

>100_bases
TCCTAACGGCTCTCGTGCCTGAAACTCTAGTAATATCTTCTCAAAGCTTGTACAATTCTGCGCCCATATTAGCGGTGATG
TTATTTATCAGAGAGGCAAC

Downstream 100 bases:

>100_bases
TAAAATGGAGTGCAGCATGGTTAGCAATTGGATTGTTAACCAGTGCCGGTTGTGTCACGGTTAATGAAGCCGATGAGATG
ACCGACGATGAGATAATTCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTTTKTNLLDFDRKGLRAFFSEELGEKAFRADQIMKWMYHFGCDDFDQMNNINKKLREKLKQKCEIRAPYVSEAQHSVDG
TIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALACTFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLMPSLDIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGKVDVALAISLHAPTDKLRSEIMPINDR
WNIEAFLACVREYIASSNANRGRVTVEYVLLDHINDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFM
KTLMEYDFTVTVRRTRGDDIDAACGQLVGDVIDRTKRTQVKQQGEAIPVKLFNLDVLQQGKAE

Sequences:

>Translated_383_residues
MTTTKTNLLDFDRKGLRAFFSEELGEKAFRADQIMKWMYHFGCDDFDQMNNINKKLREKLKQKCEIRAPYVSEAQHSVDG
TIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALACTFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLMPSLDIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGKVDVALAISLHAPTDKLRSEIMPINDR
WNIEAFLACVREYIASSNANRGRVTVEYVLLDHINDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFM
KTLMEYDFTVTVRRTRGDDIDAACGQLVGDVIDRTKRTQVKQQGEAIPVKLFNLDVLQQGKAE
>Mature_382_residues
TTTKTNLLDFDRKGLRAFFSEELGEKAFRADQIMKWMYHFGCDDFDQMNNINKKLREKLKQKCEIRAPYVSEAQHSVDGT
IKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALACTFCSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITN
VVMMGMGEPLLNMKNLMPSLDIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGKVDVALAISLHAPTDKLRSEIMPINDRW
NIEAFLACVREYIASSNANRGRVTVEYVLLDHINDDMDHARQLAELLKDTPAKINLIPFNPYPGSPYKKPSNSRIDRFMK
TLMEYDFTVTVRRTRGDDIDAACGQLVGDVIDRTKRTQVKQQGEAIPVKLFNLDVLQQGKAE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=367, Percent_Identity=71.1171662125341, Blast_Score=554, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ALISL (B6EGY4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262243.1
- GeneID:   6988888
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0726
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 43154; Mature: 43023

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTKTNLLDFDRKGLRAFFSEELGEKAFRADQIMKWMYHFGCDDFDQMNNINKKLREKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KQKCEIRAPYVSEAQHSVDGTIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALACTF
HHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCHHHHHHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLMPS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHCCH
LDIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGKVDVALAISLHAPTDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHCCCCCCC
WNIEAFLACVREYIASSNANRGRVTVEYVLLDHINDDMDHARQLAELLKDTPAKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFMKTLMEYDFTVTVRRTRGDDIDAACGQLVGDVIDRTKRTQV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQQGEAIPVKLFNLDVLQQGKAE
HHCCCCCEEEEEEHHHHHCCCCC
>Mature Secondary Structure 
TTTKTNLLDFDRKGLRAFFSEELGEKAFRADQIMKWMYHFGCDDFDQMNNINKKLREKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KQKCEIRAPYVSEAQHSVDGTIKWAMKVGDQDVETVYIPDGDRATLCVSSQVGCALACTF
HHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCHHHHHHH
CSTAQQGFNRNLKVSEIVGQIWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLMPS
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHCCH
LDIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGKVDVALAISLHAPTDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHCCCCCCC
WNIEAFLACVREYIASSNANRGRVTVEYVLLDHINDDMDHARQLAELLKDTPAKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFMKTLMEYDFTVTVRRTRGDDIDAACGQLVGDVIDRTKRTQV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQQGEAIPVKLFNLDVLQQGKAE
HHCCCCCEEEEEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA