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The map label for this gene is queF

Identifier: 209694287

GI number: 209694287

Start: 785945

End: 786790

Strand: Reverse

Name: queF

Synonym: VSAL_I0698

Alternate gene names: 209694287

Gene position: 786790-785945 (Counterclockwise)

Preceding gene: 209694290

Following gene: 209694286

Centisome position: 23.66

GC content: 37.59

Gene sequence:

>846_bases
ATGACTAAATACACGAACGCTGATGAGCTAAAATCACTGACTCTTGGCCAAAAAACTGAATACAAACACAGCTATGAGCC
AGAGTTGCTGCAAGCAGTTCCTCGCAGTTTAAACCGCGATGATCTAGCTCTTGGTGATGAATTACCTTTTGTTGGTTGTG
ATGTATGGACACTCTATGAACTGTCTTGGTTGAACCAAAATGGTTTACCTCAAGTTGCCATCGGGGACGTTGTATTACCT
GCAACAAGCCCTAATTTAGTTGAATCTAAGTCCTTTAAATTGTATTTAAACAGCTTTAACCAAACTAAATTTTCATCATG
GGAAGACGTAACCAGTACGCTAATTAAAGATTTAAGCGTATGTGCTGGTGAAGAAGTTATGGTTAACATCCACCCTGTTC
AAGCTTACTCACAACAACCTATTGTCGATATGCAAGGTGAATGTATTGATAACCAAGACATTGTTATTGATAGCTATGAG
TTTGATGCTAACTATTTAGAATCAAGTACATCAGAAAAAATGGTCAATGAAGTTCTGCATAGCCATCTACTAAAATCAAA
TTGTCTGATCACCAATCAACCAGACTGGGGCAGTGTTGAAATTAATTACGAGGGCAATAAAATTGATCATGAAAAATTGC
TTCGCTACTTAATTTCGTTCCGCCAACATAATGAGTTTCATGAGCAATGTGTTGAACGTATTTATACGGATATTATGAAA
TTCTGCTCACCAAAATCATTAACGGTATTTGCTCGTTACACTCGTCGTGGCGGATTAGATATTAACCCATTCCGCAGTTC
TCATTTAATTAAGCCTGAACATAATCTACGTTTGGCTCGCCAATAA

Upstream 100 bases:

>100_bases
CCTAATAAGACAAAAATTCAAGTAAACTAGCGTCAATTGCATCAAATAACGCTAATTTACTAAGCGACCTTTATTCTAAT
CTACTTTTGGCATTTTCAAT

Downstream 100 bases:

>100_bases
GAAATAACACGAAAAGCACATATTTATCGTCTAATTTGTCTACTTTGAACCGTTTTCTTAGTAGACAAATTTCATGACAA
CGATAGTATGTAATAGTTGT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTKYTNADELKSLTLGQKTEYKHSYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAIGDVVLP
ATSPNLVESKSFKLYLNSFNQTKFSSWEDVTSTLIKDLSVCAGEEVMVNIHPVQAYSQQPIVDMQGECIDNQDIVIDSYE
FDANYLESSTSEKMVNEVLHSHLLKSNCLITNQPDWGSVEINYEGNKIDHEKLLRYLISFRQHNEFHEQCVERIYTDIMK
FCSPKSLTVFARYTRRGGLDINPFRSSHLIKPEHNLRLARQ

Sequences:

>Translated_281_residues
MTKYTNADELKSLTLGQKTEYKHSYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAIGDVVLP
ATSPNLVESKSFKLYLNSFNQTKFSSWEDVTSTLIKDLSVCAGEEVMVNIHPVQAYSQQPIVDMQGECIDNQDIVIDSYE
FDANYLESSTSEKMVNEVLHSHLLKSNCLITNQPDWGSVEINYEGNKIDHEKLLRYLISFRQHNEFHEQCVERIYTDIMK
FCSPKSLTVFARYTRRGGLDINPFRSSHLIKPEHNLRLARQ
>Mature_280_residues
TKYTNADELKSLTLGQKTEYKHSYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAIGDVVLPA
TSPNLVESKSFKLYLNSFNQTKFSSWEDVTSTLIKDLSVCAGEEVMVNIHPVQAYSQQPIVDMQGECIDNQDIVIDSYEF
DANYLESSTSEKMVNEVLHSHLLKSNCLITNQPDWGSVEINYEGNKIDHEKLLRYLISFRQHNEFHEQCVERIYTDIMKF
CSPKSLTVFARYTRRGGLDINPFRSSHLIKPEHNLRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=283, Percent_Identity=61.13074204947, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ALISL (B6EGG7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262215.1
- ProteinModelPortal:   B6EGG7
- GeneID:   6986609
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0698
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32286; Mature: 32155

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYTNADELKSLTLGQKTEYKHSYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYE
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHE
LSWLNQNGLPQVAIGDVVLPATSPNLVESKSFKLYLNSFNQTKFSSWEDVTSTLIKDLSV
EHCCCCCCCCCEEECCEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGEEVMVNIHPVQAYSQQPIVDMQGECIDNQDIVIDSYEFDANYLESSTSEKMVNEVLH
CCCCEEEEEEEECHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHH
SHLLKSNCLITNQPDWGSVEINYEGNKIDHEKLLRYLISFRQHNEFHEQCVERIYTDIMK
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FCSPKSLTVFARYTRRGGLDINPFRSSHLIKPEHNLRLARQ
HCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
TKYTNADELKSLTLGQKTEYKHSYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYE
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHE
LSWLNQNGLPQVAIGDVVLPATSPNLVESKSFKLYLNSFNQTKFSSWEDVTSTLIKDLSV
EHCCCCCCCCCEEECCEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGEEVMVNIHPVQAYSQQPIVDMQGECIDNQDIVIDSYEFDANYLESSTSEKMVNEVLH
CCCCEEEEEEEECHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHH
SHLLKSNCLITNQPDWGSVEINYEGNKIDHEKLLRYLISFRQHNEFHEQCVERIYTDIMK
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FCSPKSLTVFARYTRRGGLDINPFRSSHLIKPEHNLRLARQ
HCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA