Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 209694280

GI number: 209694280

Start: 776939

End: 777847

Strand: Direct

Name: gcvA [H]

Synonym: VSAL_I0691

Alternate gene names: 209694280

Gene position: 776939-777847 (Clockwise)

Preceding gene: 209694277

Following gene: 209694281

Centisome position: 23.37

GC content: 41.14

Gene sequence:

>909_bases
ATGTCTCGAAGATTACCACCGTTAAATTCTCTGCGTGTGTTTGAAGCGGCAGCTCGACACCTAAGTTTCACCCGTGCAGC
AGAAGAATTATTTGTTACGCAGGCTGCGGTTAGCCATCAAATCAAAGCGTTAGAAGAATTTCTAGGGCTTAAGCTTTTTC
GTCGTCGTAATCGTTCTTTACTTCTAACAGAGGAAGGGCAAGGTTACTTTTTAGATATCAAAGAGATTTTTTCATCTCTA
TCTGAAGCAACAGATAAAGTATTGGAAAGAAGTGCGAAAGGCGCGTTAACCATTAGTTTACCGCCAAGCTTCGCAATTCA
ATGGTTAGTTCCTCGTTTGTCTGATTTTAATCAGAAAGAACCAGATATTGATGTGCGAATTAAAGCCGTTGATATGGATG
AAGGCTCATTAACAGAAGATGTTGATGTTGCGATTTATTACGGTCGAGGTAATTGGCCGGGGTTAAGAGTTGAACTGTTG
TATCAAGAGCAATTATTACCGCTGTGTTCACCTCAAGTGTTATTGAACGAAAAGCCATTAGCGACGATTGATGATCTTCG
ATTCCATACGTTATTGCATGATACCTCACGTAAAGATTGGAAGCTGTTTGTTAAACAGTTTGAATTACAAGGTATGAATG
TAAACCAAGGCCCAATTTTTAGTCACTCTACCATGGTGTTGCAAGCCGCGGCACACGGACAGGGCATTGCATTAGGTAAT
AATGTATTAGCTCAACCAGAATTAGATGCAGGTCGACTCGTTGCCCCATTTGAAGAATTACTTATTAGTAAAAATGCATT
TTATCTGGTGTGTAATGAAAAGCAGGCCGATACGGGTCGTATTGCCACCTTTAGAGAGTGGGTGCTAAGCAAAGCACGCA
GTGAGCAAGATGTGGTGGATGATGAATAG

Upstream 100 bases:

>100_bases
TAAACTGCAAAGCAATAGTAGGTTTTATCTGGTAGTTCGACAATGGACAATTTATAATAGTTAGCATTAAAAAAACTAAT
GATTCAGAATGTGAGTTCTT

Downstream 100 bases:

>100_bases
TCGTCTATGCTTTCTTATTGCAGGCTTAAGTGGGGCATTAACCGTGGGTTTAGGCGCTTTTGGATCTCATGGTTTAAAAG
ACGTATTATCACCGTATCTC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQGYFLDIKEIFSSL
SEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNQKEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELL
YQEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKLFVKQFELQGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPELDAGRLVAPFEELLISKNAFYLVCNEKQADTGRIATFREWVLSKARSEQDVVDDE

Sequences:

>Translated_302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQGYFLDIKEIFSSL
SEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNQKEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELL
YQEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKLFVKQFELQGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPELDAGRLVAPFEELLISKNAFYLVCNEKQADTGRIATFREWVLSKARSEQDVVDDE
>Mature_301_residues
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSLLLTEEGQGYFLDIKEIFSSLS
EATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNQKEPDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLY
QEQLLPLCSPQVLLNEKPLATIDDLRFHTLLHDTSRKDWKLFVKQFELQGMNVNQGPIFSHSTMVLQAAAHGQGIALGNN
VLAQPELDAGRLVAPFEELLISKNAFYLVCNEKQADTGRIATFREWVLSKARSEQDVVDDE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=73.0639730639731, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.6122448979592, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=35.0694444444444, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=27.4305555555556, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.7397260273973, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI157672245, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=28.3216783216783, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=34.6153846153846, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=204, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=24.5791245791246, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=27.7456647398844, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789440, Length=275, Percent_Identity=25.8181818181818, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=205, Percent_Identity=26.8292682926829, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87082132, Length=159, Percent_Identity=26.4150943396226, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34122; Mature: 33991

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQGYFLDIKEIFSSLSEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNQKE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLYQEQLLPLCSPQVLLNEKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEHHHCCCCCCCHHEECCCCC
ATIDDLRFHTLLHDTSRKDWKLFVKQFELQGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEECC
NVLAQPELDAGRLVAPFEELLISKNAFYLVCNEKQADTGRIATFREWVLSKARSEQDVVD
CEECCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DE
CC
>Mature Secondary Structure 
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQGYFLDIKEIFSSLSEATDKVLERSAKGALTISLPPSFAIQWLVPRLSDFNQKE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTEDVDVAIYYGRGNWPGLRVELLYQEQLLPLCSPQVLLNEKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEEHHHCCCCCCCHHEECCCCC
ATIDDLRFHTLLHDTSRKDWKLFVKQFELQGMNVNQGPIFSHSTMVLQAAAHGQGIALGN
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEECC
NVLAQPELDAGRLVAPFEELLISKNAFYLVCNEKQADTGRIATFREWVLSKARSEQDVVD
CEECCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]