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The map label for this gene is clpB [H]

Identifier: 209694256

GI number: 209694256

Start: 738205

End: 740790

Strand: Direct

Name: clpB [H]

Synonym: VSAL_I0666

Alternate gene names: 209694256

Gene position: 738205-740790 (Clockwise)

Preceding gene: 209694255

Following gene: 209694261

Centisome position: 22.2

GC content: 36.5

Gene sequence:

>2586_bases
ATGCGTTTAGACCGATTTACCAGTAAATTCCAAATCGCCATTTCAGATGCTCAATCATTAGCATTAGGTCAAGATCATCA
ATATATTGAGCCGACTCATTTAATGGTGGCTTTACTAAATCAAAACGGTAGCACGATAAGACCATTATTGACGTTACTTA
ATGTTGATGTTACCCAATTACGCTCAAAATTAACGGAAATTTTAGATAAAGCCCCAAAAGTAATTGGTATCGGTGGTGAT
GTTCAACTTTCATCAAATATGGGGGTTATTTTTAATTTGTGTGACAAAATTGCACAAAAAAGAAAAGACACTTATATCTC
TTCTGAAATATTCATGCTTGCCGCGATTGAAGACAAAGGCCCATTAGGTCATTTATTAAAAGAGTCAGGTCTTACCGAAG
CTAAAATTTCAAAATCTATTGATGAGATCCGTGGTGGTGAGAAAGTAAATGATCCTAATGCTGAAGAAAAACGTCAGGCA
TTAGAGAAGTTTACTATCGATTTAACTGAGCAAGCTGAGCAAGGTAAATTAGATCCTGTTATTGGGCGAGATGATGAAAT
TCGACGAACAATTCAAGTGCTACAACGAAGAACTAAGAATAACCCTGTAATTATAGGTCAACCCGGTGTAGGTAAAACGG
CGATTGTTGAAGGATTAGCTCAACGAATTATTAATGGCGAAGTACCCGAAGGGTTAAAGCATAAAAGAGTTCTTTCGTTA
GATATTGGGTCTTTAATTGCAGGAGCTAAATTTCGAGGTGAATTCGAAGAGCGTTTAAAAGCCGTATTAAATGAATTAGC
AAAAGAAGAAGGTAATATTATTCTTTTTATAGATGAAGTTCATACGATGGTTGGTGCTGGTAAAGGTGAAGGGTCGATGG
ATGCCGGGAATATGCTAAAGCCGGCGTTAGCTCGAGGAGACTTACATTGTGTTGGAGCGACAACGTTAGATGAATACCGT
AAATATATAGAAAAAGATCCTGCATTAGAACGACGCTTTCAAAAAGTTATTGTTGATGAGCCAACGGTAGAAGATACGAT
CGCTATATTACGTGGCTTGAAAGAACGCTATGAGATACATCATCATGTCGAAATTACCGATCCAGCAATAGTTGCTGCTG
CGACATTATCACATCGATATATTTCAGATAGACAGTTACCAGATAAAGCGATTGATTTAATCGATGAAGCGGCATCTAGT
ATTCGTATGGAAATCGATTCTAAACCAGAATCATTAGATAAACTTGAACGTAAGATCATTCAATTAAAAATAGAACAACA
AGCTTTAGTTAAAGAAAGTGATGATGCGAGTTTAAAGCGTTTAGACTCTTTAAATTTAGAATTAAGAGAAAAAGAAAGAG
ATTACTCTGAATTAGAAGAAATTTGGAAAGCTGAAAAAGCAGCACTTTCTGGAACTCAACATATAAAAACAGAGTTGGAC
ATCGCAAGAAGTAATATGGATATCGCTCGTAGAGCCGGTGATTTGAATCGTATGTCTGAATTACAATATGGCCGAATTCC
TGAATTAGAAAAACAATTGGATTTAGCTGCCCAAGCTGAAATGCAAGAAATGAGTTTGCTTAAGAATAAGGTAACAGACG
CTGAAATAGCTGAAGTTCTTTCCCGTCAAACTGGAATACCAGTTAATAAAATGTTGGAAGGAGAAAGAGATAAGCTATTA
AAGATGGAAGCCATGTTGCACCATCGAGTTATCGGTCAAGATGAAGCGGTTGAAGCGGTATCAAATGCGATTCGTCGTAG
TCGAGCTGGTTTATCGGATCCTAATCGTCCTATTGGATCTTTTTTATTCTTGGGTCCTACGGGGGTGGGGAAAACCGAAT
TATGTAAATCATTAGCAAATTTTATGTTTGATAGTGAAGACGCAATGGTCCGAATTGATATGTCGGAGTTTATGGAGAAG
CATTCTGTTGCTCGATTAGTTGGCGCACCTCCTGGCTATGTAGGTTATGAAGAGGGGGGGTATTTAACTGAAGCCGTAAG
GCGTAAGCCATATTCAGTTTTACTTTTAGATGAAGTTGAAAAGGCTCATCCAGATGTATTTAATATATTATTACAAGTAT
TAGATGATGGGCGTTTAACTGATGGTCAAGGACGTACTGTCGATTTTAAAAATACAGTAATAATTATGACATCAAATTTA
GGTTCAGAAAAAATACAACAGCATTTTGGTGAACTCGATTATGGCGGTATAAAAGAACTTGTCATGGATGTAGTTGGTCA
ACATTTTAGACCTGAATTTTTAAATAGAGTTGATGAAGCGGTTGTTTTCCATCCATTGGGACAGGAGAACATTAAAAGTA
TTGCCAAAATACAATTGCAACGTTTAGAAAAACGACTGAATGAAAAAGATTATCAGTTAAAAGTGAGTAATGAAGCGTTA
ACGTTAATATCGGAAGCTGGTTTTGATCCTGTATATGGTGCAAGACCATTAAAAAGAGCGATTCAAACTTATATTGAAAA
TCCGCTAGCACAAGATATTTTAAGCGGTAAGTTAAGGACAGGGGAAGAAATAAAACTAAAAGTAAGAAATGGTCAGCTGA
TTGCTACTCAAAATGATGATTTTTGA

Upstream 100 bases:

>100_bases
GAATAATCCCTTTTATTGCCACTCCCATCAGTTGATTTCTAAAACAATAAACCCCATTTAATAAGTAATAGTTACTTGCT
TTTCTCTTTAGGAGAGGGTT

Downstream 100 bases:

>100_bases
TAGTAAAATAATCACTTCGATGTAAAGTTGAGCACTTAACCTTTTTTGCTATAAAAAGAGTTGCATGATAGCAAAGACTC
TCTATAATTCGCCCTCACTG

Product: chaperone ClpB (heat-shock protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MRLDRFTSKFQIAISDAQSLALGQDHQYIEPTHLMVALLNQNGSTIRPLLTLLNVDVTQLRSKLTEILDKAPKVIGIGGD
VQLSSNMGVIFNLCDKIAQKRKDTYISSEIFMLAAIEDKGPLGHLLKESGLTEAKISKSIDEIRGGEKVNDPNAEEKRQA
LEKFTIDLTEQAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DIGSLIAGAKFRGEFEERLKAVLNELAKEEGNIILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDPALERRFQKVIVDEPTVEDTIAILRGLKERYEIHHHVEITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLERKIIQLKIEQQALVKESDDASLKRLDSLNLELREKERDYSELEEIWKAEKAALSGTQHIKTELD
IARSNMDIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEAMLHHRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELDYGGIKELVMDVVGQHFRPEFLNRVDEAVVFHPLGQENIKSIAKIQLQRLEKRLNEKDYQLKVSNEAL
TLISEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRTGEEIKLKVRNGQLIATQNDDF

Sequences:

>Translated_861_residues
MRLDRFTSKFQIAISDAQSLALGQDHQYIEPTHLMVALLNQNGSTIRPLLTLLNVDVTQLRSKLTEILDKAPKVIGIGGD
VQLSSNMGVIFNLCDKIAQKRKDTYISSEIFMLAAIEDKGPLGHLLKESGLTEAKISKSIDEIRGGEKVNDPNAEEKRQA
LEKFTIDLTEQAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DIGSLIAGAKFRGEFEERLKAVLNELAKEEGNIILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDPALERRFQKVIVDEPTVEDTIAILRGLKERYEIHHHVEITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLERKIIQLKIEQQALVKESDDASLKRLDSLNLELREKERDYSELEEIWKAEKAALSGTQHIKTELD
IARSNMDIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEAMLHHRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELDYGGIKELVMDVVGQHFRPEFLNRVDEAVVFHPLGQENIKSIAKIQLQRLEKRLNEKDYQLKVSNEAL
TLISEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRTGEEIKLKVRNGQLIATQNDDF
>Mature_861_residues
MRLDRFTSKFQIAISDAQSLALGQDHQYIEPTHLMVALLNQNGSTIRPLLTLLNVDVTQLRSKLTEILDKAPKVIGIGGD
VQLSSNMGVIFNLCDKIAQKRKDTYISSEIFMLAAIEDKGPLGHLLKESGLTEAKISKSIDEIRGGEKVNDPNAEEKRQA
LEKFTIDLTEQAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DIGSLIAGAKFRGEFEERLKAVLNELAKEEGNIILFIDEVHTMVGAGKGEGSMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDPALERRFQKVIVDEPTVEDTIAILRGLKERYEIHHHVEITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPESLDKLERKIIQLKIEQQALVKESDDASLKRLDSLNLELREKERDYSELEEIWKAEKAALSGTQHIKTELD
IARSNMDIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVLSRQTGIPVNKMLEGERDKLL
KMEAMLHHRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEK
HSVARLVGAPPGYVGYEEGGYLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
GSEKIQQHFGELDYGGIKELVMDVVGQHFRPEFLNRVDEAVVFHPLGQENIKSIAKIQLQRLEKRLNEKDYQLKVSNEAL
TLISEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRTGEEIKLKVRNGQLIATQNDDF

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=310, Percent_Identity=37.4193548387097, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=75.3792298716453, Blast_Score=1345, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=488, Percent_Identity=35.4508196721311, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=713, Percent_Identity=52.1739130434783, Blast_Score=761, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=866, Percent_Identity=42.7251732101617, Blast_Score=668, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96292; Mature: 96292

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFTSKFQIAISDAQSLALGQDHQYIEPTHLMVALLNQNGSTIRPLLTLLNVDVTQL
CCCHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
RSKLTEILDKAPKVIGIGGDVQLSSNMGVIFNLCDKIAQKRKDTYISSEIFMLAAIEDKG
HHHHHHHHHCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGHLLKESGLTEAKISKSIDEIRGGEKVNDPNAEEKRQALEKFTIDLTEQAEQGKLDPV
CHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DIGSLIAGAKFRGEFEERLKAVLNELAKEEGNIILFIDEVHTMVGAGKGEGSMDAGNMLK
EHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRKYIEKDPALERRFQKVIVDEPTVEDTIAILRGLKERYEIH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPESLDKLERKII
HEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
QLKIEQQALVKESDDASLKRLDSLNLELREKERDYSELEEIWKAEKAALSGTQHIKTELD
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
IARSNMDIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVL
HHHCCHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRQTGIPVNKMLEGERDKLLKMEAMLHHRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSEKIQQHFGELDYGGIKELVMDVVGQHFRPEFLNRVDEAVVFHPLGQENIKSIAKIQLQ
CHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLEKRLNEKDYQLKVSNEALTLISEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRT
HHHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCC
GEEIKLKVRNGQLIATQNDDF
CCEEEEEEECCEEEEECCCCC
>Mature Secondary Structure
MRLDRFTSKFQIAISDAQSLALGQDHQYIEPTHLMVALLNQNGSTIRPLLTLLNVDVTQL
CCCHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
RSKLTEILDKAPKVIGIGGDVQLSSNMGVIFNLCDKIAQKRKDTYISSEIFMLAAIEDKG
HHHHHHHHHCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PLGHLLKESGLTEAKISKSIDEIRGGEKVNDPNAEEKRQALEKFTIDLTEQAEQGKLDPV
CHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVIIGQPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DIGSLIAGAKFRGEFEERLKAVLNELAKEEGNIILFIDEVHTMVGAGKGEGSMDAGNMLK
EHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRKYIEKDPALERRFQKVIVDEPTVEDTIAILRGLKERYEIH
HHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPESLDKLERKII
HEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
QLKIEQQALVKESDDASLKRLDSLNLELREKERDYSELEEIWKAEKAALSGTQHIKTELD
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
IARSNMDIARRAGDLNRMSELQYGRIPELEKQLDLAAQAEMQEMSLLKNKVTDAEIAEVL
HHHCCHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRQTGIPVNKMLEGERDKLLKMEAMLHHRVIGQDEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLANFMFDSEDAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVLLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIIMTSNL
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSEKIQQHFGELDYGGIKELVMDVVGQHFRPEFLNRVDEAVVFHPLGQENIKSIAKIQLQ
CHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
RLEKRLNEKDYQLKVSNEALTLISEAGFDPVYGARPLKRAIQTYIENPLAQDILSGKLRT
HHHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCC
GEEIKLKVRNGQLIATQNDDF
CCEEEEEEECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]