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The map label for this gene is slt [H]

Identifier: 209694248

GI number: 209694248

Start: 730675

End: 732609

Strand: Direct

Name: slt [H]

Synonym: VSAL_I0658

Alternate gene names: 209694248

Gene position: 730675-732609 (Clockwise)

Preceding gene: 209694245

Following gene: 209694249

Centisome position: 21.97

GC content: 36.85

Gene sequence:

>1935_bases
ATGAAATATAGCGGACATAAATTAGGGATGATGGCGGCGTTATGTATTGGTATGAGTGTGCATACTGCTGAAGCGTTATC
AATTGATGAATCACGAGTTCAATATCAAGAAGCAAAAGATTTACTCACTGAAAAAAAATTCGATGAATATAAGAAAGTTA
GAGCTTCACTGGATGATTATCCACTCGCTCCTTATTTAGACTATCGTGAGCTGAAAATGGATTTACAGGCCAAAACTCCC
GATGACATTCGACTGTTTAGACACGCTCATCAAACACTCGCCATTGGTAATTCATTAGATTATCAATACTTAGTTATTCT
TGGTCTTAATGAGCGTTGGGCTGATTTTGTTGATTATTTTCCACAAGAACCGAGCAGTACAAATTTACAATGCTATTACT
ACCAAGCAAAAAATAAGTTGGGCGATAAAAAAACAGCGTGGAAAGGCGCTGAAAAATTGTGGTTAACGGGCAGTTCGGTT
ACTTATGAATGTGATGATTTATTTAGTGCTTGGAGTGACGCAGGAAAACTGACGGATGAACTGATTATTGAGCGTATGTT
GTTAGTATATAAAGCTCGAAATAATAATTTGTTTTCTTATTTAGAAAAAATGTTGTCTTCAGAGAAAGCAAAAATACAGG
CAAAGCATATTGTTGAGTTATTTAATGAACCTGATTCGTTAAATGAGTACTCTGCAAAAAATGAAGCCACTGAATTTACT
AAGCAACTAACGCTTATTTCTATAGAAAGAGAAGCAAGAAAAAACCCTAAAAAAGCGATTGAAATGTTGCCTGAATTAGT
GAAAAAGCAACAATTCAGTTCAGATGAACATATTGCGATAAAGAAACGAATAGTCAGTAGCATCATGTCTACAGATTCGG
CTTCTTTAGCTGCTTGGCGTGATACTGAGTTAGAAACAAATCCAGAAGATTCATTTGTAGAGCGACGAATTAGGGTTGCA
ATAAGAGCCGCTGATTGGAATGACGTTAGTAAATGGATCGGTTATTTAACACCAAGTGCAAAAAAATCGTTACGTTGGCA
GTTTTGGATTGCTCGTATAGAAAGCGAAGAGGGAAAACAATCAGCCGCTGATGCTCGCCTCAAAAAGCTACTTGGTCAGC
GTGATTTTTATAGTGTTGCGGCTGCGAATATTTTAGGTGAACCGATTCAATATTCTATGGATAAAGCATCAAAGAATACT
AAAGCCGTCGCCGGAAAGTATAAAAATGAATTAAATCGAATTAAAGAACTTATTGCGATTGATGAAATCTCAACAGCCAA
AAGTGAGTGGGAATATTTATTAGTCCGCTCTTCTAAAAAAGAAGTAAAGGAGTTAGCAAGCTATGCTGCTCAGCACCGTT
GGCATCACTTTACAGTGCTTGCAACCATTAAAGGGAAGATGTGGGGTTACTTAAGTTTACGATTTCCTATTGCTCATCAG
TGGTGGTTTGGGCATTACAGTAAAGAGTTGGGTTTAGATAAAATAACGCTTATGTCTTTGTCTCGCCAAGAAAGTGCGTT
ATACGCAGAAGCTCAATCTCCGGTTGGGGCGCGAGGATTAATGCAAATTATGCCAGCGACGGCAAAATATACAGCAAAGA
AAATTGATTATGATAAATATGAGGGTGTGAGTAGCCTTAATAATGTGGATGTGAATATACACATTGGTTCTAATTATCTA
AAAGGATTGTTAGATGATTATGATGGAAATCGAATTTTTGCTTTAGCGGGATATAATGCAGGACCTCATCGAGTTAAGCG
TTGGAGAGCGGTATCTAATGAGAAATTAGATGCCTATGCCTTTATTGAAGCGATTCCTTTTAAAGAAACAAGAGGTTATG
TACAAAACATTTTAATGTTTGAAACGTATTACCGTAGCTTACTTGGGGAGAAAGGCCCTTTCTTATCTGTTAAAGAGCTT
AATTTAGAGTATTAA

Upstream 100 bases:

>100_bases
GTAATATAAGTAAACTAAAAGAAGAATTAGAGAGTTATTTACTCTGTCTTATATCAGTATGCTGATGCAAAAGGTTGGAA
GTTAGGTGTTTAAAAAATCA

Downstream 100 bases:

>100_bases
TTTTTTTAGATTAATTATAGAGTAGGGGACTTTAGAGTCCCCTTTCTTTTATCATTATATTGAGGATTGTATGTCTGGTT
CAGCCAAATATTCAGATTGG

Product: soluble lytic murein transglycosylase precursor

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MKYSGHKLGMMAALCIGMSVHTAEALSIDESRVQYQEAKDLLTEKKFDEYKKVRASLDDYPLAPYLDYRELKMDLQAKTP
DDIRLFRHAHQTLAIGNSLDYQYLVILGLNERWADFVDYFPQEPSSTNLQCYYYQAKNKLGDKKTAWKGAEKLWLTGSSV
TYECDDLFSAWSDAGKLTDELIIERMLLVYKARNNNLFSYLEKMLSSEKAKIQAKHIVELFNEPDSLNEYSAKNEATEFT
KQLTLISIEREARKNPKKAIEMLPELVKKQQFSSDEHIAIKKRIVSSIMSTDSASLAAWRDTELETNPEDSFVERRIRVA
IRAADWNDVSKWIGYLTPSAKKSLRWQFWIARIESEEGKQSAADARLKKLLGQRDFYSVAAANILGEPIQYSMDKASKNT
KAVAGKYKNELNRIKELIAIDEISTAKSEWEYLLVRSSKKEVKELASYAAQHRWHHFTVLATIKGKMWGYLSLRFPIAHQ
WWFGHYSKELGLDKITLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVSSLNNVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSNEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGPFLSVKEL
NLEY

Sequences:

>Translated_644_residues
MKYSGHKLGMMAALCIGMSVHTAEALSIDESRVQYQEAKDLLTEKKFDEYKKVRASLDDYPLAPYLDYRELKMDLQAKTP
DDIRLFRHAHQTLAIGNSLDYQYLVILGLNERWADFVDYFPQEPSSTNLQCYYYQAKNKLGDKKTAWKGAEKLWLTGSSV
TYECDDLFSAWSDAGKLTDELIIERMLLVYKARNNNLFSYLEKMLSSEKAKIQAKHIVELFNEPDSLNEYSAKNEATEFT
KQLTLISIEREARKNPKKAIEMLPELVKKQQFSSDEHIAIKKRIVSSIMSTDSASLAAWRDTELETNPEDSFVERRIRVA
IRAADWNDVSKWIGYLTPSAKKSLRWQFWIARIESEEGKQSAADARLKKLLGQRDFYSVAAANILGEPIQYSMDKASKNT
KAVAGKYKNELNRIKELIAIDEISTAKSEWEYLLVRSSKKEVKELASYAAQHRWHHFTVLATIKGKMWGYLSLRFPIAHQ
WWFGHYSKELGLDKITLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVSSLNNVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSNEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGPFLSVKEL
NLEY
>Mature_644_residues
MKYSGHKLGMMAALCIGMSVHTAEALSIDESRVQYQEAKDLLTEKKFDEYKKVRASLDDYPLAPYLDYRELKMDLQAKTP
DDIRLFRHAHQTLAIGNSLDYQYLVILGLNERWADFVDYFPQEPSSTNLQCYYYQAKNKLGDKKTAWKGAEKLWLTGSSV
TYECDDLFSAWSDAGKLTDELIIERMLLVYKARNNNLFSYLEKMLSSEKAKIQAKHIVELFNEPDSLNEYSAKNEATEFT
KQLTLISIEREARKNPKKAIEMLPELVKKQQFSSDEHIAIKKRIVSSIMSTDSASLAAWRDTELETNPEDSFVERRIRVA
IRAADWNDVSKWIGYLTPSAKKSLRWQFWIARIESEEGKQSAADARLKKLLGQRDFYSVAAANILGEPIQYSMDKASKNT
KAVAGKYKNELNRIKELIAIDEISTAKSEWEYLLVRSSKKEVKELASYAAQHRWHHFTVLATIKGKMWGYLSLRFPIAHQ
WWFGHYSKELGLDKITLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKYEGVSSLNNVDVNIHIGSNYL
KGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSNEKLDAYAFIEAIPFKETRGYVQNILMFETYYRSLLGEKGPFLSVKEL
NLEY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=636, Percent_Identity=35.2201257861635, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74016; Mature: 74016

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSGHKLGMMAALCIGMSVHTAEALSIDESRVQYQEAKDLLTEKKFDEYKKVRASLDDY
CCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLAPYLDYRELKMDLQAKTPDDIRLFRHAHQTLAIGNSLDYQYLVILGLNERWADFVDYF
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCEEEEEEEECCHHHHHHHHHC
PQEPSSTNLQCYYYQAKNKLGDKKTAWKGAEKLWLTGSSVTYECDDLFSAWSDAGKLTDE
CCCCCCCCEEEEEEECCCCCCCCHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
LIIERMLLVYKARNNNLFSYLEKMLSSEKAKIQAKHIVELFNEPDSLNEYSAKNEATEFT
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHH
KQLTLISIEREARKNPKKAIEMLPELVKKQQFSSDEHIAIKKRIVSSIMSTDSASLAAWR
HHHHHEEECHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
DTELETNPEDSFVERRIRVAIRAADWNDVSKWIGYLTPSAKKSLRWQFWIARIESEEGKQ
CCCCCCCCCHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHEEEEEEEECCCCCCHH
SAADARLKKLLGQRDFYSVAAANILGEPIQYSMDKASKNTKAVAGKYKNELNRIKELIAI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DEISTAKSEWEYLLVRSSKKEVKELASYAAQHRWHHFTVLATIKGKMWGYLSLRFPIAHQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEEECCCHHH
WWFGHYSKELGLDKITLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCHHH
EGVSSLNNVDVNIHIGSNYLKGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSNEKLDAYA
CCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHH
FIEAIPFKETRGYVQNILMFETYYRSLLGEKGPFLSVKELNLEY
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKYSGHKLGMMAALCIGMSVHTAEALSIDESRVQYQEAKDLLTEKKFDEYKKVRASLDDY
CCCCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLAPYLDYRELKMDLQAKTPDDIRLFRHAHQTLAIGNSLDYQYLVILGLNERWADFVDYF
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCEEEEEEEECCHHHHHHHHHC
PQEPSSTNLQCYYYQAKNKLGDKKTAWKGAEKLWLTGSSVTYECDDLFSAWSDAGKLTDE
CCCCCCCCEEEEEEECCCCCCCCHHHHCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHH
LIIERMLLVYKARNNNLFSYLEKMLSSEKAKIQAKHIVELFNEPDSLNEYSAKNEATEFT
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHH
KQLTLISIEREARKNPKKAIEMLPELVKKQQFSSDEHIAIKKRIVSSIMSTDSASLAAWR
HHHHHEEECHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
DTELETNPEDSFVERRIRVAIRAADWNDVSKWIGYLTPSAKKSLRWQFWIARIESEEGKQ
CCCCCCCCCHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHEEEEEEEECCCCCCHH
SAADARLKKLLGQRDFYSVAAANILGEPIQYSMDKASKNTKAVAGKYKNELNRIKELIAI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DEISTAKSEWEYLLVRSSKKEVKELASYAAQHRWHHFTVLATIKGKMWGYLSLRFPIAHQ
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEEECCCHHH
WWFGHYSKELGLDKITLMSLSRQESALYAEAQSPVGARGLMQIMPATAKYTAKKIDYDKY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCHHH
EGVSSLNNVDVNIHIGSNYLKGLLDDYDGNRIFALAGYNAGPHRVKRWRAVSNEKLDAYA
CCCCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHH
FIEAIPFKETRGYVQNILMFETYYRSLLGEKGPFLSVKELNLEY
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]