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The map label for this gene is codB [H]

Identifier: 209694221

GI number: 209694221

Start: 695453

End: 696706

Strand: Reverse

Name: codB [H]

Synonym: VSAL_I0630

Alternate gene names: 209694221

Gene position: 696706-695453 (Counterclockwise)

Preceding gene: 209694222

Following gene: 209694220

Centisome position: 20.95

GC content: 42.26

Gene sequence:

>1254_bases
ATGTCGCATGACAATGATTTTAGCTTAGGGCCAGTACCTAAGACGGCCAGAAAAGGCATAGTATCCCTAACCCTAGTAAT
GCTAGGCCTTACTTTTTTCTCGGCAAGCATGTGGACAGGTGGAGCCCTTGGTACTGGACTCTCTTTTAATGATTTTTTCC
TCGCCGTTCTTATTGGTAATTTAATTCTCGGTATATACACTTCTTTTCTTGGTTACATTGGGGCTTCTACTGGCCTTTCT
ACTCATCTCCTCGCTCGATTCTCATTTGGTTCTAAAGGTTCTTGGCTCCCTTCTTTACTCCTTGGCGGAACCCAAGTCGG
TTGGTTTGGTGTTGGTGTTGGTGTTGGTGTTGCCATGTTTGCCATCCCGGTACAAAAAGCCACAGGTATTGATACAAACA
CCCTGATTGTAATTTCAGGTCTATTAATGACAGTGACTGTCTATTTTGGTATTTCAGCGTTGATGATTCTTTCAATGATT
GCTGTTCCTGCTATTTCAATACTTGGTGGTTACTCAGTGTTAACCGCCGTCGATAGCGTCGGTGGTATTAGCGAATTACA
AAAAATTCAACCAACCGAACCGATGGACTTCTCTATCGCACTCGCCATGGTCGTCGGTTCTTTTATTAGTGCAGGCACAT
TAACCGCTGATTTTGTTCGATTTGGAAAAAAACCAATGGGGGCCGTTGCGATTACAATGATTGCTTTCTTTATTGGTAAC
TCCCTTATGTTTATCTTTGGTGCCGCAGGCGCATCCGTTACTGGCCAATCAGATATATCTGAAGTCATGATCGCACAAGG
ACTGTTGATCCCTGCCATCATTGTTTTAGGATTAAATATCTGGACGACAAACGATAACGCCCTTTACGCATCAGGCCTTG
GTTTCTCAAATATCACCGGATTACCAAGTAAATATGTCTCAATGTTGAATGGTTTAATCGGTACCATTTGTGCCTTATGG
TTATATAACAACTTTGTTGGGTGGTTAACTTTCTTATCATTAGCCATTCCACCAATTGGTGGGGTAATTATTGCGGATTT
CTTCCGTAACCGTAAACGCTACCATGATTTTAATAATGCCAAGTTTAAAACCATTAACTGGGCAGGTATCATAGCCGTCG
TTATTGGCGTTGCCGCCGGTAAATTCCTACCTGGAATCGTTCCTTTGAACGCCGTATTAGGTGGCGCATTAGGTTACTTA
TTGCTGAACCCAATTTTAAATAAAGAACCAGCGTCTAAAACAGAAATAGCATAG

Upstream 100 bases:

>100_bases
CAAGCATTTACCTTATTGACATTACTATTATTTTCTTTAGACTCCCGTCCGTTTATCAAAATTACGATCAAAATTAACTT
AACATCGACGAGGATCTAAC

Downstream 100 bases:

>100_bases
AAGAATTATCATGACTGATTTATTAATTAAAAATATCGCACTACGAGATCAAGAAGGTTTATTCCAAATCTTGATTCAAG
ATGGTCAATTTACAGCCATT

Product: cytosine permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSHDNDFSLGPVPKTARKGIVSLTLVMLGLTFFSASMWTGGALGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLS
THLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVGVGVAMFAIPVQKATGIDTNTLIVISGLLMTVTVYFGISALMILSMI
AVPAISILGGYSVLTAVDSVGGISELQKIQPTEPMDFSIALAMVVGSFISAGTLTADFVRFGKKPMGAVAITMIAFFIGN
SLMFIFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTICALW
LYNNFVGWLTFLSLAIPPIGGVIIADFFRNRKRYHDFNNAKFKTINWAGIIAVVIGVAAGKFLPGIVPLNAVLGGALGYL
LLNPILNKEPASKTEIA

Sequences:

>Translated_417_residues
MSHDNDFSLGPVPKTARKGIVSLTLVMLGLTFFSASMWTGGALGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLS
THLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVGVGVAMFAIPVQKATGIDTNTLIVISGLLMTVTVYFGISALMILSMI
AVPAISILGGYSVLTAVDSVGGISELQKIQPTEPMDFSIALAMVVGSFISAGTLTADFVRFGKKPMGAVAITMIAFFIGN
SLMFIFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTICALW
LYNNFVGWLTFLSLAIPPIGGVIIADFFRNRKRYHDFNNAKFKTINWAGIIAVVIGVAAGKFLPGIVPLNAVLGGALGYL
LLNPILNKEPASKTEIA
>Mature_416_residues
SHDNDFSLGPVPKTARKGIVSLTLVMLGLTFFSASMWTGGALGTGLSFNDFFLAVLIGNLILGIYTSFLGYIGASTGLST
HLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVGVGVAMFAIPVQKATGIDTNTLIVISGLLMTVTVYFGISALMILSMIA
VPAISILGGYSVLTAVDSVGGISELQKIQPTEPMDFSIALAMVVGSFISAGTLTADFVRFGKKPMGAVAITMIAFFIGNS
LMFIFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITGLPSKYVSMLNGLIGTICALWL
YNNFVGWLTFLSLAIPPIGGVIIADFFRNRKRYHDFNNAKFKTINWAGIIAVVIGVAAGKFLPGIVPLNAVLGGALGYLL
LNPILNKEPASKTEIA

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=417, Percent_Identity=73.1414868105516, Blast_Score=600, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6324645, Length=431, Percent_Identity=25.754060324826, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 43605; Mature: 43474

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDNDFSLGPVPKTARKGIVSLTLVMLGLTFFSASMWTGGALGTGLSFNDFFLAVLIGN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LILGIYTSFLGYIGASTGLSTHLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVGVGVAMF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
AIPVQKATGIDTNTLIVISGLLMTVTVYFGISALMILSMIAVPAISILGGYSVLTAVDSV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGISELQKIQPTEPMDFSIALAMVVGSFISAGTLTADFVRFGKKPMGAVAITMIAFFIGN
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
SLMFIFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITG
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCCCCCC
LPSKYVSMLNGLIGTICALWLYNNFVGWLTFLSLAIPPIGGVIIADFFRNRKRYHDFNNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
KFKTINWAGIIAVVIGVAAGKFLPGIVPLNAVLGGALGYLLLNPILNKEPASKTEIA
EEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHDNDFSLGPVPKTARKGIVSLTLVMLGLTFFSASMWTGGALGTGLSFNDFFLAVLIGN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LILGIYTSFLGYIGASTGLSTHLLARFSFGSKGSWLPSLLLGGTQVGWFGVGVGVGVAMF
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
AIPVQKATGIDTNTLIVISGLLMTVTVYFGISALMILSMIAVPAISILGGYSVLTAVDSV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGISELQKIQPTEPMDFSIALAMVVGSFISAGTLTADFVRFGKKPMGAVAITMIAFFIGN
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
SLMFIFGAAGASVTGQSDISEVMIAQGLLIPAIIVLGLNIWTTNDNALYASGLGFSNITG
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCCCCCCC
LPSKYVSMLNGLIGTICALWLYNNFVGWLTFLSLAIPPIGGVIIADFFRNRKRYHDFNNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
KFKTINWAGIIAVVIGVAAGKFLPGIVPLNAVLGGALGYLLLNPILNKEPASKTEIA
EEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]