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The map label for this gene is truB

Identifier: 209694192

GI number: 209694192

Start: 663141

End: 664097

Strand: Direct

Name: truB

Synonym: VSAL_I0600

Alternate gene names: 209694192

Gene position: 663141-664097 (Clockwise)

Preceding gene: 209694191

Following gene: 209694193

Centisome position: 19.94

GC content: 41.07

Gene sequence:

>957_bases
ATGGCTCGTCGTCGTAAGGGTCGTCCTATAGATGGTGTTATCTTAATTGATAAACCGGCAGGAATTACGTCGAACGATAC
ATTGCAAAAAGTAAAACGCATCTACTTTGCAGAGAAAGCGGGCCATACTGGTGCGCTTGATCCTCTTGCTACCGGTATGC
TGCCTCTTTGTTTTGGTGAAGCGACGAAGTTTTCTCAGTTCTTATTGGATTCAGACAAGCGTTATCGTGTGGTTGCTAAA
TTAGGTGAACGCACAAATACATCTGACTCTGATGGTGAAGTGGTTGAAACTCGTGAAGTAAAAGTTGACCGTGGTCAGCT
TGAACGTTGCATTGCCAAGTTTCGTGGAACAACCGATCAAATTCCATCAATGTTTTCTGCGTTAAAACATGAAGGTCGCC
CTTTGTATGAATACGCACGTGAAGGCATTGAAGTTCCTCGTAAGTCTCGAAAAATTACGGTTTATTCAATTGAATTAATC
CGTTTTGAAGGTCATGAAGTTGAAATGGAAGTGCATTGTTCAAAAGGCACTTACATTCGAACAATAACGGATGATCTTGG
CGAAATGTTGGGTTGTGGTGCTCACGTAACGTATTTACGCCGCACTGGGGTGTCAAATTACCCATATGAAAATATGGTGA
CGATTGAACACCTTGAAGCATTATTAGAGAAAGCACATCGTGAAGAGATTTCACCTCGTGAATTACTTGATCCTCTATTA
ATGCCTATGGATTCCGCGGTACAAGATTTACCTGAAGTAAATATGATCACAGAATTAGCCGATCATGTTTTACATGGTCA
GCCAGTTCAAGTATTTGGCGCTCCACAAGATGGCATTGTTCGCATGACATCTGGTGAAGAACGTTTATTTATTGGTATTG
GTCATATTGATGATGATGGTCGAGTTGCACCAAAGCGTTTAGTTGTATTTAGAGATGAAATCGAAGAAGACAAATAA

Upstream 100 bases:

>100_bases
AAGGTATGCGCATGTCTAACCTAGTGTCAGATGTTGTGAATACAGATAAGCGTAAAATGGCAGAATCAGGTCGTACTGAA
TCTGACGAAGGGGAAGAGTA

Downstream 100 bases:

>100_bases
GTCGCATTGAAGCAATATAATTAAGAAAGAGTAAAGGCCTTGGTGTTTTTACTCTTTTTTATTGCTTTATTATTGTTCAT
CCGTATAATTCGCTCTTCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MARRRKGRPIDGVILIDKPAGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPLCFGEATKFSQFLLDSDKRYRVVAK
LGERTNTSDSDGEVVETREVKVDRGQLERCIAKFRGTTDQIPSMFSALKHEGRPLYEYAREGIEVPRKSRKITVYSIELI
RFEGHEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTYLRRTGVSNYPYENMVTIEHLEALLEKAHREEISPRELLDPLL
MPMDSAVQDLPEVNMITELADHVLHGQPVQVFGAPQDGIVRMTSGEERLFIGIGHIDDDGRVAPKRLVVFRDEIEEDK

Sequences:

>Translated_318_residues
MARRRKGRPIDGVILIDKPAGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPLCFGEATKFSQFLLDSDKRYRVVAK
LGERTNTSDSDGEVVETREVKVDRGQLERCIAKFRGTTDQIPSMFSALKHEGRPLYEYAREGIEVPRKSRKITVYSIELI
RFEGHEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTYLRRTGVSNYPYENMVTIEHLEALLEKAHREEISPRELLDPLL
MPMDSAVQDLPEVNMITELADHVLHGQPVQVFGAPQDGIVRMTSGEERLFIGIGHIDDDGRVAPKRLVVFRDEIEEDK
>Mature_317_residues
ARRRKGRPIDGVILIDKPAGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPLCFGEATKFSQFLLDSDKRYRVVAKL
GERTNTSDSDGEVVETREVKVDRGQLERCIAKFRGTTDQIPSMFSALKHEGRPLYEYAREGIEVPRKSRKITVYSIELIR
FEGHEVEMEVHCSKGTYIRTITDDLGEMLGCGAHVTYLRRTGVSNYPYENMVTIEHLEALLEKAHREEISPRELLDPLLM
PMDSAVQDLPEVNMITELADHVLHGQPVQVFGAPQDGIVRMTSGEERLFIGIGHIDDDGRVAPKRLVVFRDEIEEDK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=250, Percent_Identity=33.2, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=64.5161290322581, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17553978, Length=325, Percent_Identity=27.3846153846154, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=30.7291666666667, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=322, Percent_Identity=27.0186335403727, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281364187, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281364185, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281364183, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI62471759, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17975520, Length=234, Percent_Identity=29.9145299145299, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ALISL (B6ENE4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262120.1
- GeneID:   6989065
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0600
- HOGENOM:   HBG397258
- OMA:   ARPVQID
- ProtClustDB:   PRK05033
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35865; Mature: 35733

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRKGRPIDGVILIDKPAGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPLCFGE
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVVAKLGERTNTSDSDGEVVETREVKVDRGQLERCIAKFRGTTDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHH
IPSMFSALKHEGRPLYEYAREGIEVPRKSRKITVYSIELIRFEGHEVEMEVHCSKGTYIR
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVTYLRRTGVSNYPYENMVTIEHLEALLEKAHREEISPRELLDPLL
EHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
MPMDSAVQDLPEVNMITELADHVLHGQPVQVFGAPQDGIVRMTSGEERLFIGIGHIDDDG
CCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCC
RVAPKRLVVFRDEIEEDK
CCCCHHEEEEEHHCCCCC
>Mature Secondary Structure 
ARRRKGRPIDGVILIDKPAGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPLCFGE
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVVAKLGERTNTSDSDGEVVETREVKVDRGQLERCIAKFRGTTDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHH
IPSMFSALKHEGRPLYEYAREGIEVPRKSRKITVYSIELIRFEGHEVEMEVHCSKGTYIR
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCEEEEEEEECCCCEEE
TITDDLGEMLGCGAHVTYLRRTGVSNYPYENMVTIEHLEALLEKAHREEISPRELLDPLL
EHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
MPMDSAVQDLPEVNMITELADHVLHGQPVQVFGAPQDGIVRMTSGEERLFIGIGHIDDDG
CCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCC
RVAPKRLVVFRDEIEEDK
CCCCHHEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA