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The map label for this gene is dacB [H]

Identifier: 209694179

GI number: 209694179

Start: 647963

End: 649372

Strand: Reverse

Name: dacB [H]

Synonym: VSAL_I0587

Alternate gene names: 209694179

Gene position: 649372-647963 (Counterclockwise)

Preceding gene: 209694180

Following gene: 209694169

Centisome position: 19.53

GC content: 39.01

Gene sequence:

>1410_bases
ATGCCTTTATCTCGATTATTTATCTTATCTCTCTCGCTTTATTCTTTTTCAACCTTTGCCAGCATTCCATCAAGCATTCA
ACTCCTTCCTAGTGGGAGCCGTGCTGCTATTGTGGTTGAATCTCTACACGATCAGAGTATTGAGTTTCAACAAAAAGAAA
ATGAACTTCTCCCCCCAGCCAGTACACTAAAAGTAGCGACGGCACTTGCTTCTCGTTTAGCTCTTGGGAAAAACTTTCAA
TTTAAAACGACTTTGCATAAAACAAAACACAATAGTGTTCAATTAACCTTTTCTGGAGATCCTTCACTAACAAGAGAAAA
CCTTTCATCCCTATTTAAAAATAGCGGATTAAAAAACATTAAAGGCGATCTATGGGTTGATGATTCGATTTTCACTGGTT
ATGAGCGTGCTGTCGGCTGGCCATGGGATATTCTCGGCGTGTGTTACAGCGCACCATCAAGCGCATTAAGCATTGATGAA
AACTGTGTGCAAGCATCAATATCAACAAATGAGAACGGGACTACCCGTGTCTTCGTGCCAATACACCAACCAATTTCATT
CACAACTGCAGCTCGTACTGTCAGTGCCAAAGAGAAAAAAAATTCGTTTTGTGATTTAGAGCTAACCACTCAAGGCAATA
ACTACCACCTATCAGGCTGCTTAGTCGCAAGAAGCAAACCATTACCATTAAAGTTTGCAGTGCAAAATACAACAGAATTC
ACCGAACAAACGCTATTACGCATCCTTAATGAACAAAATATTAATTTATCTGGGAAAATAATATTCGGTAATTCGACCGG
GAAAATACAGAAAACAGTGCTGCTTGCCTCTCACCAATCAGAACCGCTAATGGTATTACTTAATCACATGTTGAAAAAAT
CAGATAACCACTACGCTGATAATTTAGTGAAAACGTTAGGCCATTATCATTACCAACAACCGGGAAGCTTTAGTAATGGC
ACCGCAGCGGTTAAAGACATCTTATTAAAACAAGCTAATGTGGATTTAACCAACGCAGTTATTGTAGATGGCTCTGGTTT
ATCCCGAAATAATAGAATGACAGCGAATCAGATGAAAAGTCTTCTTCAATTTGTCTATCGATACGATAAAGAGTTAGGGT
TAATCGCCTTACTACCGACTTCTGGTATCGATGGCACATTGATATACCGTAATAGTATGAGAAAAGAGCCAATTAAAGGC
AGCATAAAATCGAAAAGTGGCTCATTGTTTGGTAGCTATAACATGGCAGGATTTGTAGGTTTAGAAGGAAATAATCCACA
GCTATTTGTTCAGTTTGTTGCAGATTATCACCCAATAGCAGCAGAAGAAGGGGTTTTACCAGTAGAGCGACCAATTGATA
GCTTTGAGAAAGCGTTCTATCAGGCGCTAATTAAGTTATCAAACGATTGA

Upstream 100 bases:

>100_bases
GGGCGTTTTTTATTACTCCCCCCTTATTTTTATTCACTTTCCGCATACAAACCGACTCAAACCTGTAAAATGTCCTTATT
ATTTTCAGCACGAGTTGACC

Downstream 100 bases:

>100_bases
TTTTTAAGGAACACCTACATTAGCAGCAACAAAAAGCCCGAAGAAATCTCATTCTTCGGGCTTTTATCATTTAGCACTTA
AAGTATTAGTACTCGTTGTG

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MPLSRLFILSLSLYSFSTFASIPSSIQLLPSGSRAAIVVESLHDQSIEFQQKENELLPPASTLKVATALASRLALGKNFQ
FKTTLHKTKHNSVQLTFSGDPSLTRENLSSLFKNSGLKNIKGDLWVDDSIFTGYERAVGWPWDILGVCYSAPSSALSIDE
NCVQASISTNENGTTRVFVPIHQPISFTTAARTVSAKEKKNSFCDLELTTQGNNYHLSGCLVARSKPLPLKFAVQNTTEF
TEQTLLRILNEQNINLSGKIIFGNSTGKIQKTVLLASHQSEPLMVLLNHMLKKSDNHYADNLVKTLGHYHYQQPGSFSNG
TAAVKDILLKQANVDLTNAVIVDGSGLSRNNRMTANQMKSLLQFVYRYDKELGLIALLPTSGIDGTLIYRNSMRKEPIKG
SIKSKSGSLFGSYNMAGFVGLEGNNPQLFVQFVADYHPIAAEEGVLPVERPIDSFEKAFYQALIKLSND

Sequences:

>Translated_469_residues
MPLSRLFILSLSLYSFSTFASIPSSIQLLPSGSRAAIVVESLHDQSIEFQQKENELLPPASTLKVATALASRLALGKNFQ
FKTTLHKTKHNSVQLTFSGDPSLTRENLSSLFKNSGLKNIKGDLWVDDSIFTGYERAVGWPWDILGVCYSAPSSALSIDE
NCVQASISTNENGTTRVFVPIHQPISFTTAARTVSAKEKKNSFCDLELTTQGNNYHLSGCLVARSKPLPLKFAVQNTTEF
TEQTLLRILNEQNINLSGKIIFGNSTGKIQKTVLLASHQSEPLMVLLNHMLKKSDNHYADNLVKTLGHYHYQQPGSFSNG
TAAVKDILLKQANVDLTNAVIVDGSGLSRNNRMTANQMKSLLQFVYRYDKELGLIALLPTSGIDGTLIYRNSMRKEPIKG
SIKSKSGSLFGSYNMAGFVGLEGNNPQLFVQFVADYHPIAAEEGVLPVERPIDSFEKAFYQALIKLSND
>Mature_468_residues
PLSRLFILSLSLYSFSTFASIPSSIQLLPSGSRAAIVVESLHDQSIEFQQKENELLPPASTLKVATALASRLALGKNFQF
KTTLHKTKHNSVQLTFSGDPSLTRENLSSLFKNSGLKNIKGDLWVDDSIFTGYERAVGWPWDILGVCYSAPSSALSIDEN
CVQASISTNENGTTRVFVPIHQPISFTTAARTVSAKEKKNSFCDLELTTQGNNYHLSGCLVARSKPLPLKFAVQNTTEFT
EQTLLRILNEQNINLSGKIIFGNSTGKIQKTVLLASHQSEPLMVLLNHMLKKSDNHYADNLVKTLGHYHYQQPGSFSNGT
AAVKDILLKQANVDLTNAVIVDGSGLSRNNRMTANQMKSLLQFVYRYDKELGLIALLPTSGIDGTLIYRNSMRKEPIKGS
IKSKSGSLFGSYNMAGFVGLEGNNPQLFVQFVADYHPIAAEEGVLPVERPIDSFEKAFYQALIKLSND

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=478, Percent_Identity=34.7280334728033, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51619; Mature: 51488

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLFILSLSLYSFSTFASIPSSIQLLPSGSRAAIVVESLHDQSIEFQQKENELLPPA
CCCHHEEEHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCCCH
STLKVATALASRLALGKNFQFKTTLHKTKHNSVQLTFSGDPSLTRENLSSLFKNSGLKNI
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCC
KGDLWVDDSIFTGYERAVGWPWDILGVCYSAPSSALSIDENCVQASISTNENGTTRVFVP
CCCEEECCCHHCCHHHHCCCCHHHHHHHHCCCCCCEECCCHHHEEEECCCCCCCEEEEEE
IHQPISFTTAARTVSAKEKKNSFCDLELTTQGNNYHLSGCLVARSKPLPLKFAVQNTTEF
ECCCCEEHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEECCCCCCEEEEECCCHHH
TEQTLLRILNEQNINLSGKIIFGNSTGKIQKTVLLASHQSEPLMVLLNHMLKKSDNHYAD
HHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHH
NLVKTLGHYHYQQPGSFSNGTAAVKDILLKQANVDLTNAVIVDGSGLSRNNRMTANQMKS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHH
LLQFVYRYDKELGLIALLPTSGIDGTLIYRNSMRKEPIKGSIKSKSGSLFGSYNMAGFVG
HHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEE
LEGNNPQLFVQFVADYHPIAAEEGVLPVERPIDSFEKAFYQALIKLSND
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLSRLFILSLSLYSFSTFASIPSSIQLLPSGSRAAIVVESLHDQSIEFQQKENELLPPA
CCHHEEEHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCCCH
STLKVATALASRLALGKNFQFKTTLHKTKHNSVQLTFSGDPSLTRENLSSLFKNSGLKNI
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCC
KGDLWVDDSIFTGYERAVGWPWDILGVCYSAPSSALSIDENCVQASISTNENGTTRVFVP
CCCEEECCCHHCCHHHHCCCCHHHHHHHHCCCCCCEECCCHHHEEEECCCCCCCEEEEEE
IHQPISFTTAARTVSAKEKKNSFCDLELTTQGNNYHLSGCLVARSKPLPLKFAVQNTTEF
ECCCCEEHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEECCCCCCEEEEECCCHHH
TEQTLLRILNEQNINLSGKIIFGNSTGKIQKTVLLASHQSEPLMVLLNHMLKKSDNHYAD
HHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHH
NLVKTLGHYHYQQPGSFSNGTAAVKDILLKQANVDLTNAVIVDGSGLSRNNRMTANQMKS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHH
LLQFVYRYDKELGLIALLPTSGIDGTLIYRNSMRKEPIKGSIKSKSGSLFGSYNMAGFVG
HHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEE
LEGNNPQLFVQFVADYHPIAAEEGVLPVERPIDSFEKAFYQALIKLSND
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]