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The map label for this gene is mlaE [H]

Identifier: 209694101

GI number: 209694101

Start: 564510

End: 565304

Strand: Direct

Name: mlaE [H]

Synonym: VSAL_I0506

Alternate gene names: 209694101

Gene position: 564510-565304 (Clockwise)

Preceding gene: 209694100

Following gene: 209694102

Centisome position: 16.98

GC content: 43.4

Gene sequence:

>795_bases
GTGAGTAATTTCTCTTTAATCTTATTTGTTGAAAATATTGGTAAGCGAGCGCTTTCAACGTGCGAAGCTATTGGTCGAGC
GACTTTTATGCTTTTTGGGGCGATCATTGCAAAGCCTCAACCCGTAAGAATGTTTCCATTGTTATTGCAACAGCTGTATT
CAGTCGGTGTGTTATCGCTTGTTATTATCGTGGTCTCTGGGCTGTTTATCGGCATGGTGATAAGCCTTCAAGGTTATGTG
ATTTTAGTCGATTACGGTGCAGAAACCAGTCTCGGCCAATTGGTGTCACTTTCGTTATTACGTGAATTAGGCCCTGTGGT
TACCGCACTCTTATTTGCTGGGCGTGCCGGCTCTGCATTAACTGCAGAAATAGGGTTAATGAAAACGACAGAGCAGTTGT
CTAGTATGGAAATGATGGCGATAGATCCTTTGCGTCGTATTATTGCCCCGCGCTTATGGGCGGGTGTTATTTCAATGCCA
TTACTGGCGATGATTTTTATGGCTGTGGGTATATGGGGTGGTCAAATTGTTGGTGTTGAATGGAAAGGTATTGATCACGG
AAGCTTTTGGTCAGTAATGCAAGCATCGGTCGATGTACGCCACGACATTGTTAACAGTTTAATTAAGTGTGTCGTGTTTG
CGTTTGTCGTGACATGGATTGCTTTATTTAATGGCTATGATGCGACCCCAACATCAGAAGGGATCTCATTAGCAACAACA
CGAACGGTAGTGCATTCGTCACTGGCGGTATTAGGAATTGATTTTGTGCTTACAGCACTGATGTTTGGGAATTAA

Upstream 100 bases:

>100_bases
AATCCAGACCCTCAAGTACGACAATTTTTAGATGGCCGCTCTGACGGACCCGTTCCTTTTCGCTTTCCGTCGAAACCATT
AGTTGAGGAGTTATTTCCTG

Downstream 100 bases:

>100_bases
TCATGCAGCAAACACGTAAAATAGAATTATTAGTTGGATTTTTTGTTTTGGCTGGCGCGGCAGCGTTATTAATGTTGCTA
CTAAAAGTGGCCGATGTTCG

Product: toluene tolerance protein Ttg2B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSNFSLILFVENIGKRALSTCEAIGRATFMLFGAIIAKPQPVRMFPLLLQQLYSVGVLSLVIIVVSGLFIGMVISLQGYV
ILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQLSSMEMMAIDPLRRIIAPRLWAGVISMP
LLAMIFMAVGIWGGQIVGVEWKGIDHGSFWSVMQASVDVRHDIVNSLIKCVVFAFVVTWIALFNGYDATPTSEGISLATT
RTVVHSSLAVLGIDFVLTALMFGN

Sequences:

>Translated_264_residues
MSNFSLILFVENIGKRALSTCEAIGRATFMLFGAIIAKPQPVRMFPLLLQQLYSVGVLSLVIIVVSGLFIGMVISLQGYV
ILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQLSSMEMMAIDPLRRIIAPRLWAGVISMP
LLAMIFMAVGIWGGQIVGVEWKGIDHGSFWSVMQASVDVRHDIVNSLIKCVVFAFVVTWIALFNGYDATPTSEGISLATT
RTVVHSSLAVLGIDFVLTALMFGN
>Mature_263_residues
SNFSLILFVENIGKRALSTCEAIGRATFMLFGAIIAKPQPVRMFPLLLQQLYSVGVLSLVIIVVSGLFIGMVISLQGYVI
LVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSALTAEIGLMKTTEQLSSMEMMAIDPLRRIIAPRLWAGVISMPL
LAMIFMAVGIWGGQIVGVEWKGIDHGSFWSVMQASVDVRHDIVNSLIKCVVFAFVVTWIALFNGYDATPTSEGISLATTR
TVVHSSLAVLGIDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=253, Percent_Identity=72.7272727272727, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28415; Mature: 28284

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFSLILFVENIGKRALSTCEAIGRATFMLFGAIIAKPQPVRMFPLLLQQLYSVGVLSL
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VIIVVSGLFIGMVISLQGYVILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAEIGLMKTTEQLSSMEMMAIDPLRRIIAPRLWAGVISMPLLAMIFMAVGIWGGQIVGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
WKGIDHGSFWSVMQASVDVRHDIVNSLIKCVVFAFVVTWIALFNGYDATPTSEGISLATT
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RTVVHSSLAVLGIDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNFSLILFVENIGKRALSTCEAIGRATFMLFGAIIAKPQPVRMFPLLLQQLYSVGVLSL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VIIVVSGLFIGMVISLQGYVILVDYGAETSLGQLVSLSLLRELGPVVTALLFAGRAGSAL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TAEIGLMKTTEQLSSMEMMAIDPLRRIIAPRLWAGVISMPLLAMIFMAVGIWGGQIVGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
WKGIDHGSFWSVMQASVDVRHDIVNSLIKCVVFAFVVTWIALFNGYDATPTSEGISLATT
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
RTVVHSSLAVLGIDFVLTALMFGN
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]