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The map label for this gene is cafA [H]

Identifier: 209694082

GI number: 209694082

Start: 544023

End: 545492

Strand: Direct

Name: cafA [H]

Synonym: VSAL_I0487

Alternate gene names: 209694082

Gene position: 544023-545492 (Clockwise)

Preceding gene: 209694081

Following gene: 209694083

Centisome position: 16.36

GC content: 39.73

Gene sequence:

>1470_bases
ATGAGTACAGAGTTGTTAATAAACGTAACTCCGAGTGAAACTCGAGTTGCGATGATTGAAGCCGGTGTGCTTCAAGAGAT
ACATGTAGAAAGAGAAGCAAGACGCGGTATTGTGGGTAATATATACAAAGGCAGAGTAAGCCGAGTATTACCGGGGATGC
AAGCGGCGTTTATTGATATTGGCTTGGATAAAGCGGCATTTTTGCATGCTTCAGACATTGTGCCTCATACTGAATGCGTT
TCTGAGAATGAAAAGCGTCAGTTTCAGGTGCGAGATATTTCTCAGTTAGTACATCAAGGTCAAGATTTAGTGGTTCAAGT
GGTAAAAGATCCACTTGGAACAAAAGGTGCTCGTCTAACTACGGATATTACTCTTCCTTCGCGTTACTTGGTCTTTATGC
CAGGAGCGAGTCATGTTGGTGTCTCTCAACGAATTGAAAGTGAAAAAGAACGTAATCGTTTAAAGAATACGGTTTCTGAT
TATTGCGATGAGTTTGGCGGTTTTATTATTCGTACTGCGGCAGAAGGCGCGAAAGAAGCGGAGATCTCCCAAGATGCTGC
TTTTTTAAAACGTTTGTGGCATAAGGTAATTGAGCGTCGTAAAAAGAGCAAAACAAAATCAATGCTTTATGGTGAGCTTG
GATTAGATCAACGTATCTTACGTGATTTTGTCGGTACAGAGTTAGATTTAATTCGTGTCGATTCAAAGCAAGCCTTTGAA
AAATTAAAAGAATTTACAACTGAATTCGTTCCTGAATTAACGAAAAAATTGGAATATTACTCTGGTGATAAACCGATTTT
TGATATGTATGAAACAGAGAACGAAATTCAACGCGCTTTAGATCGTAAGGTCGAGTTGAAATCTGGCGGCTATTTGATCA
TAGATCAAACAGAAGCGATGACAACGGTTGATATAAATACGGGGGCCTTTGTTGGGCGTCGTAATTTAGAAGAAACGATT
TTCAACACGAATATTGAAGCAACTCAAGCCATTGCTCGACAGCTTCGTTTACGTAATCTTGGTGGGATCATTATCATTGA
TTTTATCGATATGATGAGTGAAGAACATCGTCGCCGAGTGCTGCAAGTATTAGGCGCTGCGTTAGATAAAGACCGAGTTA
AAACGAACATTAATGGTTTTACCCAATTGGGTTTAGTTGAGATGACTCGTAAACGTACGCGTGAAAGTATTGAGCATGTT
TTATGTGGTCAATGTCCTACGTGTGAAGGCCGCGGTGCAGTTAAAACGGTTGAGAGCGTTTGTTACGAGATTTTACGTGA
AATTACTCGAGTAAATAGAGCGTACGATTCTGATAAATTTGTCGTATATGCTGCTGTTGCTGTTGCTGATGCACTTCAAG
GTGAAGAATCTCATGCATTGGCAGAGCTTGAATTATTTATTGGTAAGCAAGTGAAAATCCAAGCTGAACCACTTTATATT
CAAGAGCAATTTGATGTTGTTATGATGTAA

Upstream 100 bases:

>100_bases
TCACGTATTGATGGCAGCTATCACGCTGTCATGGGATTGCCATTGATGGAAACCGATCAGCTCTTACACCAATTTTTGTA
AAAATTAGTGAGGTGCTCCA

Downstream 100 bases:

>100_bases
GAGAATGATAAGTGACCACTAAGTTAATTCGTACTGAACGTTGCTTGATGGGATTATTGCTGCTGGTATTTTTACTGGCA
GCATTAGTCATAACAAGCCT

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSTELLINVTPSETRVAMIEAGVLQEIHVEREARRGIVGNIYKGRVSRVLPGMQAAFIDIGLDKAAFLHASDIVPHTECV
SENEKRQFQVRDISQLVHQGQDLVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEKERNRLKNTVSD
YCDEFGGFIIRTAAEGAKEAEISQDAAFLKRLWHKVIERRKKSKTKSMLYGELGLDQRILRDFVGTELDLIRVDSKQAFE
KLKEFTTEFVPELTKKLEYYSGDKPIFDMYETENEIQRALDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMMSEEHRRRVLQVLGAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHV
LCGQCPTCEGRGAVKTVESVCYEILREITRVNRAYDSDKFVVYAAVAVADALQGEESHALAELELFIGKQVKIQAEPLYI
QEQFDVVMM

Sequences:

>Translated_489_residues
MSTELLINVTPSETRVAMIEAGVLQEIHVEREARRGIVGNIYKGRVSRVLPGMQAAFIDIGLDKAAFLHASDIVPHTECV
SENEKRQFQVRDISQLVHQGQDLVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEKERNRLKNTVSD
YCDEFGGFIIRTAAEGAKEAEISQDAAFLKRLWHKVIERRKKSKTKSMLYGELGLDQRILRDFVGTELDLIRVDSKQAFE
KLKEFTTEFVPELTKKLEYYSGDKPIFDMYETENEIQRALDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETI
FNTNIEATQAIARQLRLRNLGGIIIIDFIDMMSEEHRRRVLQVLGAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHV
LCGQCPTCEGRGAVKTVESVCYEILREITRVNRAYDSDKFVVYAAVAVADALQGEESHALAELELFIGKQVKIQAEPLYI
QEQFDVVMM
>Mature_488_residues
STELLINVTPSETRVAMIEAGVLQEIHVEREARRGIVGNIYKGRVSRVLPGMQAAFIDIGLDKAAFLHASDIVPHTECVS
ENEKRQFQVRDISQLVHQGQDLVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESEKERNRLKNTVSDY
CDEFGGFIIRTAAEGAKEAEISQDAAFLKRLWHKVIERRKKSKTKSMLYGELGLDQRILRDFVGTELDLIRVDSKQAFEK
LKEFTTEFVPELTKKLEYYSGDKPIFDMYETENEIQRALDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETIF
NTNIEATQAIARQLRLRNLGGIIIIDFIDMMSEEHRRRVLQVLGAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHVL
CGQCPTCEGRGAVKTVESVCYEILREITRVNRAYDSDKFVVYAAVAVADALQGEESHALAELELFIGKQVKIQAEPLYIQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=78.3231083844581, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=464, Percent_Identity=33.4051724137931, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55337; Mature: 55206

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELLINVTPSETRVAMIEAGVLQEIHVEREARRGIVGNIYKGRVSRVLPGMQAAFIDI
CCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHEEC
GLDKAAFLHASDIVPHTECVSENEKRQFQVRDISQLVHQGQDLVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESEKERNRLKNTVSDYCDEFGGFIIRTAAEGAKEA
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHH
EISQDAAFLKRLWHKVIERRKKSKTKSMLYGELGLDQRILRDFVGTELDLIRVDSKQAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCHHHHH
KLKEFTTEFVPELTKKLEYYSGDKPIFDMYETENEIQRALDRKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCEEECCCCEEEEECCCCE
TTVDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMMSEEHRRRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LQVLGAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCGQCPTCEGRGAVKTVESV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CYEILREITRVNRAYDSDKFVVYAAVAVADALQGEESHALAELELFIGKQVKIQAEPLYI
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCEEE
QEQFDVVMM
ECCCCEEEC
>Mature Secondary Structure 
STELLINVTPSETRVAMIEAGVLQEIHVEREARRGIVGNIYKGRVSRVLPGMQAAFIDI
CCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHEEC
GLDKAAFLHASDIVPHTECVSENEKRQFQVRDISQLVHQGQDLVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIESEKERNRLKNTVSDYCDEFGGFIIRTAAEGAKEA
EEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHH
EISQDAAFLKRLWHKVIERRKKSKTKSMLYGELGLDQRILRDFVGTELDLIRVDSKQAFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCHHHHH
KLKEFTTEFVPELTKKLEYYSGDKPIFDMYETENEIQRALDRKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCEEECCCCEEEEECCCCE
TTVDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMMSEEHRRRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LQVLGAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCGQCPTCEGRGAVKTVESV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CYEILREITRVNRAYDSDKFVVYAAVAVADALQGEESHALAELELFIGKQVKIQAEPLYI
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCEEE
QEQFDVVMM
ECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]