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The map label for this gene is imp [H]

Identifier: 209693974

GI number: 209693974

Start: 405399

End: 407753

Strand: Reverse

Name: imp [H]

Synonym: VSAL_I0371

Alternate gene names: 209693974

Gene position: 407753-405399 (Counterclockwise)

Preceding gene: 209693976

Following gene: 209693973

Centisome position: 12.26

GC content: 38.39

Gene sequence:

>2355_bases
ATGTCTTTTACCTCTCGCAGCTTACTTGCTTCATTTACTGGATGCCTTCTTTATGGATCCCCTGCCATTGCTGATGATGC
AGGCCAATTCATACCTCCAGAAAACAATATTATTTTCCCTTTGAAAACTGATGCAGTGAATTCTTGTCAAGTCCCTGTGA
AGATGTCAAAAGAGGAAGAAGATCAACAGCCTGTATTAATTCAAGCAGACAGTGTTGAAGCTCATAATAACTCAAAAGCC
ACCTATAAAGGTAATGTGCATGTCATAAAAGGACGCCAAGAAATAAAAGCGGACAATATTACATTGCACCAACAAGAAAA
CATCGCCATAGCCGAAGGTAATGTTGATTACTCAAGCATGGACATGAGATCAACGTCAGATAAAGTCACAACGGATTTAA
ATACCGAAGACACCACGATGATCAATACATCATATAAAATGTCTTGTCAGCCTATTCGTGGTACTGCTAGCCGTGTATTA
AAAACTGGGCAAGCTATTTATCAATTAGAAGACGCTTCATTTACTACCTGTCCTGCCGGTGATAAAAGTTGGCGCGTTAA
AGCCAGTGATATCGAATTAGATCAATCTGATGAATGGGCAACATTCTATAATGCTCGCTTTGAAATTCTTGATGTGCCTA
TTTTATATCTTCCATATATAACCGTGCCCGTCGGTGATACCCGTAAAACCGGGGTATTAATCCCAAGTGCAGGTTTAGAC
AGTAAAAATGGATTTGAATTATCCATTCCAATCTATTGGAATATTGCCCCAAACTACGATGCAACAACCACCATTAATTA
CATGGAAAAACGTGGTACCCAATTAGAAGGGAATTTCCGTTATTTAACCGATTTTGGTTCTGGTCAAGTCGATGCAGAAT
ACCTCGGTGATGATGCGAAATACAAAGAAAAAGGCGCTCGTTGGGGAGCAAGCTGGAATCACTCTGGCATTTATAAACAA
AACTGGAAATTTGAAGTTGAATACTCACAAGTCAGTGATATTACTTATTTCCAAGATTTAAACTCTAGCATTGGTACGCG
TGATGAAGGTCAGTTACAACAATCTGGTGAGGTATCTTACCGAACTCAAGATTGGGATTTAACGATGCGTGTTCGTGATT
TCCAAGTACTTGTCGAGAATAGTTATCCATATCGCTTGATGCCACAAATGGAATTTAACTATTACGCACCTCAATTTTAT
AATGAATTCGATTTCAATTTAAATAGTCATATAAGTAAATTTGAGACTGATGACCCAACAAAGCCATCAGCCACTCGTGT
ACACATGGAACCTAAATTAAGCTTACCATTAAGTGGTACTTGGTGGTCATTAACACCTGAAACGAGCTTACTTTATACTT
ATTACACTCAAGAATTTGACGCAAAAAAAGATCCTTCATTATCGGATTTAGCGGAAGAGACGGATCGGACCATTCCCGAA
GTCCGTATTAATGGTGCTATCTATTTAGACAGCACGCATAAGTTCTTAGGTAAATACTTACAAACCTTAGAACCGAAAAT
ACAATACTTATATGTACCAGAAGTCGATCAAAGTGATATTTATGGTGGTAAAGGCGAAGGTGGTTACGACAGCAGTAAAC
TACAACTAGATTACTATGGTTTATTTAGAGATCGCCAATACAGTGGTGTTGATTATATTGCGGCAGCAAATCAATTCAGT
ATTGGGGCAACGTCTCGTTTTTATGATGATACTTACAAAGAGCGAATGAACATCTCATTTGGTCAAATCCTATACATGAA
TGGATCTGGCACTGCAAATGAAGGCGATGATAAAAATTCATCGGCATGGGCGATAGAAAGTGATTTCAACTACGATGATT
ATCTTTTCTACCATGGTGGTGTTCAATACGACTCTAATGTGGACGAGCTACAGGTTGCAAACAGCACCATAGAATACCAA
CACAGCAATGGTTATGTTCAGCTTAACTATCGTTATGTAAGTAAAAACTACATCCAAAGTAACGTAAACTTTCAAGATGA
CCTAGATAAAATAACTCAACATGGTATTTACCAAGCAGGGTTTTTAACGCAGTACAACATCAATAATAGCTGGCAGCTCC
AAGCACAATATTTTCATGATACTAAAGAAGACTACATGCTTGAAGCTCTCGTCGGTGCAACCTACCTAAGTGACTGTTGG
TCATTTGGTTTAACTTATAGTGATCAGTTAATTGCACCAGAAAGCGCCGGTTCTATTGGTAGTTATGAACCAGAATATGA
AAGTAACTTAATGCTTTCTATTGCGATTCGAGGTTTAGGTACCAATACGGGTATTACATCAGGCACAGCAACCAATGCGT
TAGGCTATGGTCGCCCATTCTATTTAAATAATTAA

Upstream 100 bases:

>100_bases
GGTTTGGGCATAGCTTTTGCTTTATTATAAAGGATTATCTCTATCTAGTATCTTTGAGTATATCAGCGGATTAATATAAC
AAACAGGAACGTCTAACTCG

Downstream 100 bases:

>100_bases
TTATAGGGACACGAGTTAACGCTAACGGATATCGCTATCGTTAACTCTCTATACACTCTTGGATGACACTATGAAAAACT
GGAAAATTCCATTTATTAGC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MSFTSRSLLASFTGCLLYGSPAIADDAGQFIPPENNIIFPLKTDAVNSCQVPVKMSKEEEDQQPVLIQADSVEAHNNSKA
TYKGNVHVIKGRQEIKADNITLHQQENIAIAEGNVDYSSMDMRSTSDKVTTDLNTEDTTMINTSYKMSCQPIRGTASRVL
KTGQAIYQLEDASFTTCPAGDKSWRVKASDIELDQSDEWATFYNARFEILDVPILYLPYITVPVGDTRKTGVLIPSAGLD
SKNGFELSIPIYWNIAPNYDATTTINYMEKRGTQLEGNFRYLTDFGSGQVDAEYLGDDAKYKEKGARWGASWNHSGIYKQ
NWKFEVEYSQVSDITYFQDLNSSIGTRDEGQLQQSGEVSYRTQDWDLTMRVRDFQVLVENSYPYRLMPQMEFNYYAPQFY
NEFDFNLNSHISKFETDDPTKPSATRVHMEPKLSLPLSGTWWSLTPETSLLYTYYTQEFDAKKDPSLSDLAEETDRTIPE
VRINGAIYLDSTHKFLGKYLQTLEPKIQYLYVPEVDQSDIYGGKGEGGYDSSKLQLDYYGLFRDRQYSGVDYIAAANQFS
IGATSRFYDDTYKERMNISFGQILYMNGSGTANEGDDKNSSAWAIESDFNYDDYLFYHGGVQYDSNVDELQVANSTIEYQ
HSNGYVQLNYRYVSKNYIQSNVNFQDDLDKITQHGIYQAGFLTQYNINNSWQLQAQYFHDTKEDYMLEALVGATYLSDCW
SFGLTYSDQLIAPESAGSIGSYEPEYESNLMLSIAIRGLGTNTGITSGTATNALGYGRPFYLNN

Sequences:

>Translated_784_residues
MSFTSRSLLASFTGCLLYGSPAIADDAGQFIPPENNIIFPLKTDAVNSCQVPVKMSKEEEDQQPVLIQADSVEAHNNSKA
TYKGNVHVIKGRQEIKADNITLHQQENIAIAEGNVDYSSMDMRSTSDKVTTDLNTEDTTMINTSYKMSCQPIRGTASRVL
KTGQAIYQLEDASFTTCPAGDKSWRVKASDIELDQSDEWATFYNARFEILDVPILYLPYITVPVGDTRKTGVLIPSAGLD
SKNGFELSIPIYWNIAPNYDATTTINYMEKRGTQLEGNFRYLTDFGSGQVDAEYLGDDAKYKEKGARWGASWNHSGIYKQ
NWKFEVEYSQVSDITYFQDLNSSIGTRDEGQLQQSGEVSYRTQDWDLTMRVRDFQVLVENSYPYRLMPQMEFNYYAPQFY
NEFDFNLNSHISKFETDDPTKPSATRVHMEPKLSLPLSGTWWSLTPETSLLYTYYTQEFDAKKDPSLSDLAEETDRTIPE
VRINGAIYLDSTHKFLGKYLQTLEPKIQYLYVPEVDQSDIYGGKGEGGYDSSKLQLDYYGLFRDRQYSGVDYIAAANQFS
IGATSRFYDDTYKERMNISFGQILYMNGSGTANEGDDKNSSAWAIESDFNYDDYLFYHGGVQYDSNVDELQVANSTIEYQ
HSNGYVQLNYRYVSKNYIQSNVNFQDDLDKITQHGIYQAGFLTQYNINNSWQLQAQYFHDTKEDYMLEALVGATYLSDCW
SFGLTYSDQLIAPESAGSIGSYEPEYESNLMLSIAIRGLGTNTGITSGTATNALGYGRPFYLNN
>Mature_783_residues
SFTSRSLLASFTGCLLYGSPAIADDAGQFIPPENNIIFPLKTDAVNSCQVPVKMSKEEEDQQPVLIQADSVEAHNNSKAT
YKGNVHVIKGRQEIKADNITLHQQENIAIAEGNVDYSSMDMRSTSDKVTTDLNTEDTTMINTSYKMSCQPIRGTASRVLK
TGQAIYQLEDASFTTCPAGDKSWRVKASDIELDQSDEWATFYNARFEILDVPILYLPYITVPVGDTRKTGVLIPSAGLDS
KNGFELSIPIYWNIAPNYDATTTINYMEKRGTQLEGNFRYLTDFGSGQVDAEYLGDDAKYKEKGARWGASWNHSGIYKQN
WKFEVEYSQVSDITYFQDLNSSIGTRDEGQLQQSGEVSYRTQDWDLTMRVRDFQVLVENSYPYRLMPQMEFNYYAPQFYN
EFDFNLNSHISKFETDDPTKPSATRVHMEPKLSLPLSGTWWSLTPETSLLYTYYTQEFDAKKDPSLSDLAEETDRTIPEV
RINGAIYLDSTHKFLGKYLQTLEPKIQYLYVPEVDQSDIYGGKGEGGYDSSKLQLDYYGLFRDRQYSGVDYIAAANQFSI
GATSRFYDDTYKERMNISFGQILYMNGSGTANEGDDKNSSAWAIESDFNYDDYLFYHGGVQYDSNVDELQVANSTIEYQH
SNGYVQLNYRYVSKNYIQSNVNFQDDLDKITQHGIYQAGFLTQYNINNSWQLQAQYFHDTKEDYMLEALVGATYLSDCWS
FGLTYSDQLIAPESAGSIGSYEPEYESNLMLSIAIRGLGTNTGITSGTATNALGYGRPFYLNN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=756, Percent_Identity=36.2433862433862, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88852; Mature: 88720

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTSRSLLASFTGCLLYGSPAIADDAGQFIPPENNIIFPLKTDAVNSCQVPVKMSKEEE
CCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCC
DQQPVLIQADSVEAHNNSKATYKGNVHVIKGRQEIKADNITLHQQENIAIAEGNVDYSSM
CCCCEEEEECCEECCCCCCEEEECCEEEEECCHHCCCCCEEEEECCCEEEEECCCCCCCC
DMRSTSDKVTTDLNTEDTTMINTSYKMSCQPIRGTASRVLKTGQAIYQLEDASFTTCPAG
CCCCCCCCEEECCCCCCCEEEECCEEEEEECCCCHHHHHHHCCCEEEEECCCCEEECCCC
DKSWRVKASDIELDQSDEWATFYNARFEILDVPILYLPYITVPVGDTRKTGVLIPSAGLD
CCCEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEECEEEEECCCCCCCEEEEECCCCC
SKNGFELSIPIYWNIAPNYDATTTINYMEKRGTQLEGNFRYLTDFGSGQVDAEYLGDDAK
CCCCEEEEEEEEEEECCCCCCCEEEHHHHHCCCEECCCEEEEEECCCCCEEHHHCCCCCH
YKEKGARWGASWNHSGIYKQNWKFEVEYSQVSDITYFQDLNSSIGTRDEGQLQQSGEVSY
HHHHCCCCCCCCCCCCCEECCCEEEEEHHHCCCCHHHHHHCHHCCCCCCCCCCCCCCEEE
RTQDWDLTMRVRDFQVLVENSYPYRLMPQMEFNYYAPQFYNEFDFNLNSHISKFETDDPT
EECCCEEEEEEEEEEEEEECCCCEEEECCCCCCEECCCCCCCCCCCCHHCCHHCCCCCCC
KPSATRVHMEPKLSLPLSGTWWSLTPETSLLYTYYTQEFDAKKDPSLSDLAEETDRTIPE
CCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCE
VRINGAIYLDSTHKFLGKYLQTLEPKIQYLYVPEVDQSDIYGGKGEGGYDSSKLQLDYYG
EEECCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEE
LFRDRQYSGVDYIAAANQFSIGATSRFYDDTYKERMNISFGQILYMNGSGTANEGDDKNS
EEECCCCCCEEEEEECCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
SAWAIESDFNYDDYLFYHGGVQYDSNVDELQVANSTIEYQHSNGYVQLNYRYVSKNYIQS
CEEEEECCCCCCCEEEEECCEEECCCCCCEEECCCEEEEEECCCEEEEEEEEEECHHHHC
NVNFQDDLDKITQHGIYQAGFLTQYNINNSWQLQAQYFHDTKEDYMLEALVGATYLSDCW
CCCCHHHHHHHHHCCCEEECCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SFGLTYSDQLIAPESAGSIGSYEPEYESNLMLSIAIRGLGTNTGITSGTATNALGYGRPF
HCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCE
YLNN
EECC
>Mature Secondary Structure 
SFTSRSLLASFTGCLLYGSPAIADDAGQFIPPENNIIFPLKTDAVNSCQVPVKMSKEEE
CCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCC
DQQPVLIQADSVEAHNNSKATYKGNVHVIKGRQEIKADNITLHQQENIAIAEGNVDYSSM
CCCCEEEEECCEECCCCCCEEEECCEEEEECCHHCCCCCEEEEECCCEEEEECCCCCCCC
DMRSTSDKVTTDLNTEDTTMINTSYKMSCQPIRGTASRVLKTGQAIYQLEDASFTTCPAG
CCCCCCCCEEECCCCCCCEEEECCEEEEEECCCCHHHHHHHCCCEEEEECCCCEEECCCC
DKSWRVKASDIELDQSDEWATFYNARFEILDVPILYLPYITVPVGDTRKTGVLIPSAGLD
CCCEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEECEEEEECCCCCCCEEEEECCCCC
SKNGFELSIPIYWNIAPNYDATTTINYMEKRGTQLEGNFRYLTDFGSGQVDAEYLGDDAK
CCCCEEEEEEEEEEECCCCCCCEEEHHHHHCCCEECCCEEEEEECCCCCEEHHHCCCCCH
YKEKGARWGASWNHSGIYKQNWKFEVEYSQVSDITYFQDLNSSIGTRDEGQLQQSGEVSY
HHHHCCCCCCCCCCCCCEECCCEEEEEHHHCCCCHHHHHHCHHCCCCCCCCCCCCCCEEE
RTQDWDLTMRVRDFQVLVENSYPYRLMPQMEFNYYAPQFYNEFDFNLNSHISKFETDDPT
EECCCEEEEEEEEEEEEEECCCCEEEECCCCCCEECCCCCCCCCCCCHHCCHHCCCCCCC
KPSATRVHMEPKLSLPLSGTWWSLTPETSLLYTYYTQEFDAKKDPSLSDLAEETDRTIPE
CCCCEEEEECCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCE
VRINGAIYLDSTHKFLGKYLQTLEPKIQYLYVPEVDQSDIYGGKGEGGYDSSKLQLDYYG
EEECCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEE
LFRDRQYSGVDYIAAANQFSIGATSRFYDDTYKERMNISFGQILYMNGSGTANEGDDKNS
EEECCCCCCEEEEEECCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
SAWAIESDFNYDDYLFYHGGVQYDSNVDELQVANSTIEYQHSNGYVQLNYRYVSKNYIQS
CEEEEECCCCCCCEEEEECCEEECCCCCCEEECCCEEEEEECCCEEEEEEEEEECHHHHC
NVNFQDDLDKITQHGIYQAGFLTQYNINNSWQLQAQYFHDTKEDYMLEALVGATYLSDCW
CCCCHHHHHHHHHCCCEEECCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SFGLTYSDQLIAPESAGSIGSYEPEYESNLMLSIAIRGLGTNTGITSGTATNALGYGRPF
HCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCE
YLNN
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA