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The map label for this gene is folA [H]

Identifier: 209693969

GI number: 209693969

Start: 400556

End: 401077

Strand: Direct

Name: folA [H]

Synonym: VSAL_I0366

Alternate gene names: 209693969

Gene position: 400556-401077 (Clockwise)

Preceding gene: 209693968

Following gene: 209693975

Centisome position: 12.05

GC content: 38.7

Gene sequence:

>522_bases
ATGAAGATTAGTATGATTGCTGCCATGGCAAATAATGGGAAAATAGATAAATCGTGCAATCTGTCAAATTCTCAATCTCG
AATTATTGGCAAAAATAATCAAATGCCATGGCATTTACCTGCTGATTTTGCATGGTTTAAGCAATGTACTATGGGAAAGC
CAATTGTGATGGGGCGTAAAACCTATGAATCCATTGGTCGTCCATTACCGGGACGCTTAAATATTGTATTAAGCCGTGAT
GCGAGTTTGAAGATTGAAGGTGTCACTTGTGTGACTTCAATTGATGAAGCGAAGCAAGTCGCCGGTGATATTGAAGAACT
AATGATTATTGGTGGTGGCTCTATCTATCAAGCGTGTTTACCTGATGCTGATACTCTTTATCTTACCTTTATTGATGCTG
ATATTGATGGAGATACTCAATTCCCTGATTGGGGAGAGGGGTGGAACCAGACGCATTCTGAGATGTATTTGAAAGATGAA
AAGAATCAGTACGATATGGAATTTGTAGTGTTAGAAAGGTAA

Upstream 100 bases:

>100_bases
GTTTGCTGTTTTACCGTAGAAAATCTATAGTGTGATAAAGGTATTAATTTGTTATTCTACGGACCAGTTTTTTTGCTTAA
TAAAATGAGATTTAGAATAA

Downstream 100 bases:

>100_bases
GAGCAGGATGAACTTCGTTCTAAAGGGCGCTTCGCTTAAGGAGGCTGCGCCCTGAGGAATAAAAGCAAAGAGAAAAGCAG
GTTTCAGGAGCTCTCGCTTT

Product: dihydrofolate reductase type 3

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKISMIAAMANNGKIDKSCNLSNSQSRIIGKNNQMPWHLPADFAWFKQCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRD
ASLKIEGVTCVTSIDEAKQVAGDIEELMIIGGGSIYQACLPDADTLYLTFIDADIDGDTQFPDWGEGWNQTHSEMYLKDE
KNQYDMEFVVLER

Sequences:

>Translated_173_residues
MKISMIAAMANNGKIDKSCNLSNSQSRIIGKNNQMPWHLPADFAWFKQCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRD
ASLKIEGVTCVTSIDEAKQVAGDIEELMIIGGGSIYQACLPDADTLYLTFIDADIDGDTQFPDWGEGWNQTHSEMYLKDE
KNQYDMEFVVLER
>Mature_173_residues
MKISMIAAMANNGKIDKSCNLSNSQSRIIGKNNQMPWHLPADFAWFKQCTMGKPIVMGRKTYESIGRPLPGRLNIVLSRD
ASLKIEGVTCVTSIDEAKQVAGDIEELMIIGGGSIYQACLPDADTLYLTFIDADIDGDTQFPDWGEGWNQTHSEMYLKDE
KNQYDMEFVVLER

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=146, Percent_Identity=34.2465753424658, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1786233, Length=172, Percent_Identity=48.2558139534884, Blast_Score=163, Evalue=5e-42,
Organism=Drosophila melanogaster, GI116008026, Length=166, Percent_Identity=30.7228915662651, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647458, Length=166, Percent_Identity=30.7228915662651, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19373; Mature: 19373

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISMIAAMANNGKIDKSCNLSNSQSRIIGKNNQMPWHLPADFAWFKQCTMGKPIVMGRK
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEECCCCHHHHHHHCCCCCEEECCH
TYESIGRPLPGRLNIVLSRDASLKIEGVTCVTSIDEAKQVAGDIEELMIIGGGSIYQACL
HHHHHCCCCCCEEEEEEECCCCEEECCEEEEECHHHHHHHHCCCEEEEEECCCCEEHHHC
PDADTLYLTFIDADIDGDTQFPDWGEGWNQTHSEMYLKDEKNQYDMEFVVLER
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEC
>Mature Secondary Structure
MKISMIAAMANNGKIDKSCNLSNSQSRIIGKNNQMPWHLPADFAWFKQCTMGKPIVMGRK
CCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEECCCCHHHHHHHCCCCCEEECCH
TYESIGRPLPGRLNIVLSRDASLKIEGVTCVTSIDEAKQVAGDIEELMIIGGGSIYQACL
HHHHHCCCCCCEEEEEEECCCCEEECCEEEEECHHHHHHHHCCCEEEEEECCCCEEHHHC
PDADTLYLTFIDADIDGDTQFPDWGEGWNQTHSEMYLKDEKNQYDMEFVVLER
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]