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The map label for this gene is ispB [H]

Identifier: 209693963

GI number: 209693963

Start: 395817

End: 396788

Strand: Reverse

Name: ispB [H]

Synonym: VSAL_I0360

Alternate gene names: 209693963

Gene position: 396788-395817 (Counterclockwise)

Preceding gene: 209693970

Following gene: 209693961

Centisome position: 11.93

GC content: 40.95

Gene sequence:

>972_bases
ATGGATTTTAAAGCTATCCAAGCCCTCACGGCTGATGACATGGCAATCGTAAATGAAACCATCTTGGCACAACTAAATTC
TGATGTTGCGCTTATCAACCAACTTGGTTTTTACATTGTAAGTGGAGGCGGTAAACGTCTGCGCCCAGTTCTTGCCATTT
TATCTGCAAAAGCACTTGGATACGAAGGTAAAGACCACACAATGGCAGCCGCCTTTATTGAATTTATTCATACGGCAACA
TTACTTCATGATGATGTTGTTGATGAATCAGATATGCGTCGTGGTAAAGCCACAGCGAATGCTGAATTTGGTAATGCTGC
TAGTGTACTTGTTGGTGACTTTATCTACACTCGTTCATTCCAAATGATGACGAGCTTAGGATCAATAAAGATCCTGAGTT
TAATGAGTGAATCCGTCAATGTGATCGCAGAAGGTGAAGTGCTTCAATTAATGAACTGCAATGATCCTAATACAACTGAA
GAAAGCTACATGCAAGTGATCTATTCTAAGACTGCACGTTTATTTGAAGCTGCGACGCAGATCGGCGCGATCCTAAATAA
TGCGTCACAAGAAATCGAAACGGCTTTACAGAATTACGGTAAATATCTTGGTACCGCTTTTCAACTTATCGATGATGTCA
TGGATTACACCTCTGACGGCGAAGACATGGGTAAAAACGTTGGCGATGATCTCGCAGAAGGCAAACCAACACTTCCTTTA
TTACATGCCATGAACAACGGTAACGCTGAGCAAGCAGCAATGATCCGTCATGCTATAGAGCAAGGTAATGGACTGGATAA
ACTCGATCCTATTTTAGCCTGTATGAAAGAAGTGGGTTCACTAGAATATACAAAACAAAAAGCAGATGAAGAAGCGGACA
AAGCAATCGCTGAACTTTCTATCATTCCTGAATCTGAATACAAAGAAGCATTAAAAGCACTTGCTCATCTTGCTGTTAAA
CGCAGCAAATAA

Upstream 100 bases:

>100_bases
TAATTTTCGACTGTATTGCCTACCCTACTTTAGTGTATTATTTGTTTAATCACTTATTACATTTAAGCCTGACTTAGGCC
CTAACCTTGCTGGAATAATA

Downstream 100 bases:

>100_bases
GGCTTTTATATCAAGATAAAATAAAAGGGCTGATACTTTTAATAAGTATCAGCCCTTTTCTATTTAAAGATATCTACGTA
TGCATTAAGAATTTACTTAA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSESVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNNASQEIETALQNYGKYLGTAFQLIDDVMDYTSDGEDMGKNVGDDLAEGKPTLPL
LHAMNNGNAEQAAMIRHAIEQGNGLDKLDPILACMKEVGSLEYTKQKADEEADKAIAELSIIPESEYKEALKALAHLAVK
RSK

Sequences:

>Translated_323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSESVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNNASQEIETALQNYGKYLGTAFQLIDDVMDYTSDGEDMGKNVGDDLAEGKPTLPL
LHAMNNGNAEQAAMIRHAIEQGNGLDKLDPILACMKEVGSLEYTKQKADEEADKAIAELSIIPESEYKEALKALAHLAVK
RSK
>Mature_323_residues
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALGYEGKDHTMAAAFIEFIHTAT
LLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSFQMMTSLGSIKILSLMSESVNVIAEGEVLQLMNCNDPNTTE
ESYMQVIYSKTARLFEAATQIGAILNNASQEIETALQNYGKYLGTAFQLIDDVMDYTSDGEDMGKNVGDDLAEGKPTLPL
LHAMNNGNAEQAAMIRHAIEQGNGLDKLDPILACMKEVGSLEYTKQKADEEADKAIAELSIIPESEYKEALKALAHLAVK
RSK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=30.4347826086957, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4758430, Length=264, Percent_Identity=23.8636363636364, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI83700220, Length=264, Percent_Identity=23.8636363636364, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=70.8978328173375, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=28.0851063829787, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=346, Percent_Identity=30.9248554913295, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=32.1070234113712, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6325188, Length=288, Percent_Identity=24.3055555555556, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=300, Percent_Identity=30.6666666666667, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=274, Percent_Identity=22.992700729927, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24660002, Length=274, Percent_Identity=22.992700729927, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35052; Mature: 35052

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALG
CCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHC
YEGKDHTMAAAFIEFIHTATLLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QMMTSLGSIKILSLMSESVNVIAEGEVLQLMNCNDPNTTEESYMQVIYSKTARLFEAATQ
HHHHHHCHHHHHHHHHHHHCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILNNASQEIETALQNYGKYLGTAFQLIDDVMDYTSDGEDMGKNVGDDLAEGKPTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCHH
LHAMNNGNAEQAAMIRHAIEQGNGLDKLDPILACMKEVGSLEYTKQKADEEADKAIAELS
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IIPESEYKEALKALAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFKAIQALTADDMAIVNETILAQLNSDVALINQLGFYIVSGGGKRLRPVLAILSAKALG
CCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHC
YEGKDHTMAAAFIEFIHTATLLHDDVVDESDMRRGKATANAEFGNAASVLVGDFIYTRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QMMTSLGSIKILSLMSESVNVIAEGEVLQLMNCNDPNTTEESYMQVIYSKTARLFEAATQ
HHHHHHCHHHHHHHHHHHHCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGAILNNASQEIETALQNYGKYLGTAFQLIDDVMDYTSDGEDMGKNVGDDLAEGKPTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCHH
LHAMNNGNAEQAAMIRHAIEQGNGLDKLDPILACMKEVGSLEYTKQKADEEADKAIAELS
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IIPESEYKEALKALAHLAVKRSK
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]