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The map label for this gene is thiP [H]

Identifier: 209693954

GI number: 209693954

Start: 381775

End: 383397

Strand: Direct

Name: thiP [H]

Synonym: VSAL_I0350

Alternate gene names: 209693954

Gene position: 381775-383397 (Clockwise)

Preceding gene: 209693953

Following gene: 209693955

Centisome position: 11.48

GC content: 38.82

Gene sequence:

>1623_bases
ATGAAAACGAATCCCTTAAATTCTATTCCCTATATCGGCTATATGATCGCTACTGTGATTGTTATTTTTGTTGGCGGAGC
TATTGGTGCATTATTAATCGCGTCACCCAGTATCAATGTCAGTTTAATTTGGTCCGATCCTTATTATATTCATATAACCA
AATTTAGTTTTTATCAAGCAACCCTGTCTACGTTGTTTAGTGTGGTACTTGCGGTTCCGACAGCATATGCACTATCTCGT
CGCTCTTTTTTAGGACGCGGTCTGTTATTAAAGTTGTGTGCGACGACCTTGGTTTTACCTGTGTTAGTAGGGGTTTTTGG
TTTACTTGCTATTTATGGAAACAGCGGTCTCTTAGCTAAGGTGTTTGCATTTTTTGGTGGTAAATTGCCGTTTTCTATTT
ATGGATTAAATGGCATTTTACTCGCGCATGTGTTCTTTAATTATCCTTTTGCAACTCGTTTGTTTTTACAATCATTAGAC
AGTATACCGATGGAGCAACATCAGCTTTCTGCTCATCTAGGATTGACTGGATGGAATAAATTTAACGTTGTTGCATGGCC
AAGAGTAAAACAACAATTTCCGCATGTTGCAGGCTTGGTGTTCATGCTTTGCTTTACCAGTTTTGCAACCGTAATGGCAT
TAGGTGGAGGACCAAAATCAACCACGATTGAACTTGCTATTTACCAAGCCATTAAATTTGATTTTGATTTACAGGCCGGC
GCTATTTTAGCGTTATGGCAAATGTTGTTATGCGCAGGATTATCGCTGTGTATTCAACGGTTTTCTACGTCGATTGCCGT
TGACAGTGCAACGCTAGGTAAGCCCATTATCTATCAAGACTCAAAGAGACAAAAGTTGTGGGATAAAGGGTGGATCATAT
TACTTACTCTGTTTATTTTACCTCCATTACTCATGGTTGTTATTAGTGGGATAAATTCTGAAGTTATTACGGTGATGACC
TCTAGTGCTTTTTGGTCAGCATTCGCTAATTCATTCATTGTTGCTTTCTGTGCGGCGCTATTAGCGATGGTTTTTGGGGT
TTTATTGCTTCATACCACTCGAGCGTTAAGGCTTAATCATCAGCGTTTTTTGGCGGATAAATTAGATTTAATTGGTACGA
TGATTTTAGTTACCCCAGGATTGGTTATTAGCACGGGGTTGTTTTTGTTGCTTAGAAACATCACTGATGTATTTAGTATG
GCTATTTGGATCGTCATTTTAGTCAATGCACTTATGGCGCTTCCATACGTCATAAAGACGCTTGCTCAACCTTTATTGCA
TATGGCACAGCAATATCAGTTATTGTGTGCGAGTTTAGGACTTAATGGTTTTACTCGTTTTCGCTTAATTGAATGGAGAG
GGTTAAGAAAACCGATGGCTCATGCCTTTGCCTTTGCCTTTGCCATTGCTTTTATCTTATCAATGGGTGATTTAAGTGCG
ATAGCGCTTTTTGGTAGTCAGGATTTTAGGACATTGCCGCTATATTTATTTCAATTATTAGGCAGTTATCAAATGGAAGC
GGCTGCGGTGGTTTCACTTTGTTTACTCTTATTAAGTGTGGGTGTGTTTAGTACGGTTGAATGGCTTTTTTCATCAAAGA
ATCTACAAAGAGAGAATGTGTAA

Upstream 100 bases:

>100_bases
TGTGGATTCGCGAATGGCAACACGCATTAATCTTTTAATCGATGCACTGAAAAGAGACGTATAATACGTACGTTTCTTTT
TGGGACAGATAGATAACATC

Downstream 100 bases:

>100_bases
TGCTGACTTTTTCTAATGTAAAATATCGCTATCATTCTGAGGTTTTTGAGTTTGATATGTCAGTTAAACAAGGTGAAGTT
GTTGCTATTCTTGGGCCTAG

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKTNPLNSIPYIGYMIATVIVIFVGGAIGALLIASPSINVSLIWSDPYYIHITKFSFYQATLSTLFSVVLAVPTAYALSR
RSFLGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLLAKVFAFFGGKLPFSIYGLNGILLAHVFFNYPFATRLFLQSLD
SIPMEQHQLSAHLGLTGWNKFNVVAWPRVKQQFPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
AILALWQMLLCAGLSLCIQRFSTSIAVDSATLGKPIIYQDSKRQKLWDKGWIILLTLFILPPLLMVVISGINSEVITVMT
SSAFWSAFANSFIVAFCAALLAMVFGVLLLHTTRALRLNHQRFLADKLDLIGTMILVTPGLVISTGLFLLLRNITDVFSM
AIWIVILVNALMALPYVIKTLAQPLLHMAQQYQLLCASLGLNGFTRFRLIEWRGLRKPMAHAFAFAFAIAFILSMGDLSA
IALFGSQDFRTLPLYLFQLLGSYQMEAAAVVSLCLLLLSVGVFSTVEWLFSSKNLQRENV

Sequences:

>Translated_540_residues
MKTNPLNSIPYIGYMIATVIVIFVGGAIGALLIASPSINVSLIWSDPYYIHITKFSFYQATLSTLFSVVLAVPTAYALSR
RSFLGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLLAKVFAFFGGKLPFSIYGLNGILLAHVFFNYPFATRLFLQSLD
SIPMEQHQLSAHLGLTGWNKFNVVAWPRVKQQFPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
AILALWQMLLCAGLSLCIQRFSTSIAVDSATLGKPIIYQDSKRQKLWDKGWIILLTLFILPPLLMVVISGINSEVITVMT
SSAFWSAFANSFIVAFCAALLAMVFGVLLLHTTRALRLNHQRFLADKLDLIGTMILVTPGLVISTGLFLLLRNITDVFSM
AIWIVILVNALMALPYVIKTLAQPLLHMAQQYQLLCASLGLNGFTRFRLIEWRGLRKPMAHAFAFAFAIAFILSMGDLSA
IALFGSQDFRTLPLYLFQLLGSYQMEAAAVVSLCLLLLSVGVFSTVEWLFSSKNLQRENV
>Mature_540_residues
MKTNPLNSIPYIGYMIATVIVIFVGGAIGALLIASPSINVSLIWSDPYYIHITKFSFYQATLSTLFSVVLAVPTAYALSR
RSFLGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLLAKVFAFFGGKLPFSIYGLNGILLAHVFFNYPFATRLFLQSLD
SIPMEQHQLSAHLGLTGWNKFNVVAWPRVKQQFPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
AILALWQMLLCAGLSLCIQRFSTSIAVDSATLGKPIIYQDSKRQKLWDKGWIILLTLFILPPLLMVVISGINSEVITVMT
SSAFWSAFANSFIVAFCAALLAMVFGVLLLHTTRALRLNHQRFLADKLDLIGTMILVTPGLVISTGLFLLLRNITDVFSM
AIWIVILVNALMALPYVIKTLAQPLLHMAQQYQLLCASLGLNGFTRFRLIEWRGLRKPMAHAFAFAFAIAFILSMGDLSA
IALFGSQDFRTLPLYLFQLLGSYQMEAAAVVSLCLLLLSVGVFSTVEWLFSSKNLQRENV

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=478, Percent_Identity=46.652719665272, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59409; Mature: 59409

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNPLNSIPYIGYMIATVIVIFVGGAIGALLIASPSINVSLIWSDPYYIHITKFSFYQA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEHHHHHH
TLSTLFSVVLAVPTAYALSRRSFLGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLLAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VFAFFGGKLPFSIYGLNGILLAHVFFNYPFATRLFLQSLDSIPMEQHQLSAHLGLTGWNK
HHHHHCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
FNVVAWPRVKQQFPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
CCEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCC
AILALWQMLLCAGLSLCIQRFSTSIAVDSATLGKPIIYQDSKRQKLWDKGWIILLTLFIL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCCCCEECCCHHHHHHHCHHHHHHHHHHH
PPLLMVVISGINSEVITVMTSSAFWSAFANSFIVAFCAALLAMVFGVLLLHTTRALRLNH
HHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRFLADKLDLIGTMILVTPGLVISTGLFLLLRNITDVFSMAIWIVILVNALMALPYVIKT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQPLLHMAQQYQLLCASLGLNGFTRFRLIEWRGLRKPMAHAFAFAFAIAFILSMGDLSA
HHHHHHHHHHHHHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
IALFGSQDFRTLPLYLFQLLGSYQMEAAAVVSLCLLLLSVGVFSTVEWLFSSKNLQRENV
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTNPLNSIPYIGYMIATVIVIFVGGAIGALLIASPSINVSLIWSDPYYIHITKFSFYQA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEHHHHHH
TLSTLFSVVLAVPTAYALSRRSFLGRGLLLKLCATTLVLPVLVGVFGLLAIYGNSGLLAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VFAFFGGKLPFSIYGLNGILLAHVFFNYPFATRLFLQSLDSIPMEQHQLSAHLGLTGWNK
HHHHHCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
FNVVAWPRVKQQFPHVAGLVFMLCFTSFATVMALGGGPKSTTIELAIYQAIKFDFDLQAG
CCEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCC
AILALWQMLLCAGLSLCIQRFSTSIAVDSATLGKPIIYQDSKRQKLWDKGWIILLTLFIL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCCCCEECCCHHHHHHHCHHHHHHHHHHH
PPLLMVVISGINSEVITVMTSSAFWSAFANSFIVAFCAALLAMVFGVLLLHTTRALRLNH
HHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRFLADKLDLIGTMILVTPGLVISTGLFLLLRNITDVFSMAIWIVILVNALMALPYVIKT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQPLLHMAQQYQLLCASLGLNGFTRFRLIEWRGLRKPMAHAFAFAFAIAFILSMGDLSA
HHHHHHHHHHHHHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
IALFGSQDFRTLPLYLFQLLGSYQMEAAAVVSLCLLLLSVGVFSTVEWLFSSKNLQRENV
EEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]