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The map label for this gene is tbpA [H]

Identifier: 209693953

GI number: 209693953

Start: 380696

End: 381712

Strand: Direct

Name: tbpA [H]

Synonym: VSAL_I0349

Alternate gene names: 209693953

Gene position: 380696-381712 (Clockwise)

Preceding gene: 209693952

Following gene: 209693954

Centisome position: 11.45

GC content: 38.94

Gene sequence:

>1017_bases
ATGAAAACACGTTATATATCCCCTCTACTTAAAACACTACCTATCGCTTTAGCTGTTAGTTCTCTCTCTTCTTTTTCTGC
TTCTGCTTCTGAAACGCTAACAGTCTATACTTATGATTCTTTTGCTGCTGATTGGGGCCCTGGTCCAACAATTAAAGCGG
CTTTTGAAAAACAGTGTGGCTGCAATCTTGATCTTGTCGCTTTGGATGATGGCGTTTCTATTTTGAATCGTCTTCGTTTA
GAAGGCAGTAATTCAAAAGCGGACATTGTTTTAGGGCTCGATAATAATTTAATGGCAGAGGCGAAAGTAACGGGTTTACT
GGCTGAACATAATGTAAATACGGACTCAGTGACAATACCTAATGGTTGGAATGATAAGACCTTTGTTCCTTATGATTATG
GTTATTTTGCTTTCGTATATAACAAAGAAAAAGTAAAGAACCCACCTAAGAGTCTAAAAGAATTAGTAGAACAACGAGAT
GATTTAACCGTTATTTATCAAGATCCAAGAACCTCTACACCGGGCCAAGGTTTATTGTTATGGATGAAATCGGTGTATGG
AGATGATGTTGCTCAAGCATGGAAACAACTTGCAAAGAAAACCGTCACCGTCACGAAAGGCTGGTCAGAAGCTTACAGTA
TGTTTTTAGAGGGTGAATCTGATCTTGTTTTGTCTTATACGACGTCTCCAGCATACCATTCACTTGCTGAAAAAGACGAT
CGATTTGCAGCGGCTAATTTTTCAGAAGGTCATTACATGCAAGTGGAAGTGGCGGCGAAAGTAAAAAATACGCAACATAA
TGCGTTAACTGATGAGTTCTTAGCTTTTATTCTTAGTAAGGAATTTCAATCAGCGATGCCAACGGGAAATTGGATGTATC
CAGTGACTAATATTACGTTACCTAAAGGGTTTGAAGACCTTAAGGTTCCGACTAAAGCGTTGTCATTTAGTTCTGCGGAG
ATTGCGAAACAAAGAAAGTTGTGGATTCGCGAATGGCAACACGCATTAATCTTTTAA

Upstream 100 bases:

>100_bases
ACTACCTGAATCAGATAATGCTGACGTAGGGATTGAGATGACATTCTCATCGAGTATATCAACTTAACCCTGTGTCGCTC
AAAAGGACAAAGAGCGCATA

Downstream 100 bases:

>100_bases
TCGATGCACTGAAAAGAGACGTATAATACGTACGTTTCTTTTTGGGACAGATAGATAACATCATGAAAACGAATCCCTTA
AATTCTATTCCCTATATCGG

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKTRYISPLLKTLPIALAVSSLSSFSASASETLTVYTYDSFAADWGPGPTIKAAFEKQCGCNLDLVALDDGVSILNRLRL
EGSNSKADIVLGLDNNLMAEAKVTGLLAEHNVNTDSVTIPNGWNDKTFVPYDYGYFAFVYNKEKVKNPPKSLKELVEQRD
DLTVIYQDPRTSTPGQGLLLWMKSVYGDDVAQAWKQLAKKTVTVTKGWSEAYSMFLEGESDLVLSYTTSPAYHSLAEKDD
RFAAANFSEGHYMQVEVAAKVKNTQHNALTDEFLAFILSKEFQSAMPTGNWMYPVTNITLPKGFEDLKVPTKALSFSSAE
IAKQRKLWIREWQHALIF

Sequences:

>Translated_338_residues
MKTRYISPLLKTLPIALAVSSLSSFSASASETLTVYTYDSFAADWGPGPTIKAAFEKQCGCNLDLVALDDGVSILNRLRL
EGSNSKADIVLGLDNNLMAEAKVTGLLAEHNVNTDSVTIPNGWNDKTFVPYDYGYFAFVYNKEKVKNPPKSLKELVEQRD
DLTVIYQDPRTSTPGQGLLLWMKSVYGDDVAQAWKQLAKKTVTVTKGWSEAYSMFLEGESDLVLSYTTSPAYHSLAEKDD
RFAAANFSEGHYMQVEVAAKVKNTQHNALTDEFLAFILSKEFQSAMPTGNWMYPVTNITLPKGFEDLKVPTKALSFSSAE
IAKQRKLWIREWQHALIF
>Mature_338_residues
MKTRYISPLLKTLPIALAVSSLSSFSASASETLTVYTYDSFAADWGPGPTIKAAFEKQCGCNLDLVALDDGVSILNRLRL
EGSNSKADIVLGLDNNLMAEAKVTGLLAEHNVNTDSVTIPNGWNDKTFVPYDYGYFAFVYNKEKVKNPPKSLKELVEQRD
DLTVIYQDPRTSTPGQGLLLWMKSVYGDDVAQAWKQLAKKTVTVTKGWSEAYSMFLEGESDLVLSYTTSPAYHSLAEKDD
RFAAANFSEGHYMQVEVAAKVKNTQHNALTDEFLAFILSKEFQSAMPTGNWMYPVTNITLPKGFEDLKVPTKALSFSSAE
IAKQRKLWIREWQHALIF

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=308, Percent_Identity=68.1818181818182, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37660; Mature: 37660

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRYISPLLKTLPIALAVSSLSSFSASASETLTVYTYDSFAADWGPGPTIKAAFEKQCG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHCCC
CNLDLVALDDGVSILNRLRLEGSNSKADIVLGLDNNLMAEAKVTGLLAEHNVNTDSVTIP
CCEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHEEHHHCCCCCCEEECC
NGWNDKTFVPYDYGYFAFVYNKEKVKNPPKSLKELVEQRDDLTVIYQDPRTSTPGQGLLL
CCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
WMKSVYGDDVAQAWKQLAKKTVTVTKGWSEAYSMFLEGESDLVLSYTTSPAYHSLAEKDD
HHHHHCCCHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCC
RFAAANFSEGHYMQVEVAAKVKNTQHNALTDEFLAFILSKEFQSAMPTGNWMYPVTNITL
CEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
PKGFEDLKVPTKALSFSSAEIAKQRKLWIREWQHALIF
CCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTRYISPLLKTLPIALAVSSLSSFSASASETLTVYTYDSFAADWGPGPTIKAAFEKQCG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHCCC
CNLDLVALDDGVSILNRLRLEGSNSKADIVLGLDNNLMAEAKVTGLLAEHNVNTDSVTIP
CCEEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCEEEHHHHHEEHHHCCCCCCEEECC
NGWNDKTFVPYDYGYFAFVYNKEKVKNPPKSLKELVEQRDDLTVIYQDPRTSTPGQGLLL
CCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
WMKSVYGDDVAQAWKQLAKKTVTVTKGWSEAYSMFLEGESDLVLSYTTSPAYHSLAEKDD
HHHHHCCCHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCC
RFAAANFSEGHYMQVEVAAKVKNTQHNALTDEFLAFILSKEFQSAMPTGNWMYPVTNITL
CEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
PKGFEDLKVPTKALSFSSAEIAKQRKLWIREWQHALIF
CCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]