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The map label for this gene is tuf [H]

Identifier: 209693919

GI number: 209693919

Start: 357073

End: 358257

Strand: Direct

Name: tuf [H]

Synonym: VSAL_I0313

Alternate gene names: 209693919

Gene position: 357073-358257 (Clockwise)

Preceding gene: 209693918

Following gene: 209693920

Centisome position: 10.74

GC content: 44.05

Gene sequence:

>1185_bases
GTGTCTAAAGAAAAATTTGAACGTACGAAACCGCACGTAAACGTTGGTACAATCGGCCACGTTGACCACGGTAAAACAAC
TCTAACTGCTGCTATCTGTACTACACTTGCAAAAATTTACGGTGGCGTAGCTAAAGATTTCGCATCAATCGATAACGCTC
CTGAAGAGCGCGAGCGTGGTATCACAATCTCAACTTCTCACGTTGAGTACGATACTCCTGCACGTCACTATGCACACGTT
GACTGTCCTGGACACGCCGATTATGTTAAAAACATGATCACTGGTGCTGCTCAAATGGATGGTGGTATCCTAGTTGTTGC
TGCAACTGATGGCCCTATGCCACAAACACGTGAGCACATCCTACTTGGTCGTCAAGTTGGTATTCCTTTCATCGTTGTGT
TCATGAACAAATGTGACATGGTTGATGATGAAGAACTTCTTGAGCTAGTAGAAATGGAAGTTCGTGAACTTCTTTCTGAA
TACGATTACCCAGGTGATGATTTACCAGTAATCCAAGGTTCAGCTCTTGGCGCTCTAAACGGCGAAAAACAATGGGAAGA
TAAAATCATTGAGCTTGCAGAAGCTCTAGATACATATATTCCTCTTCCTGAACGTGCTGTTGACATGCCTTTCCTACTTC
CTATTGAAGATGTATTCTCAATTCAAGGTCGTGGTACAGTTGTTACTGGTCGTATCGAACGTGGTATTCTACGTGTAGGC
GACGAAGTAGAAATCATTGGTATCAAAGAAACTACAGTTTCTACATGTACTGGTGTTGAAATGTTCCGTAAACTGCTTGA
CGAAGGTCGTGCTGGTGAGAACGTTGGTGCACTTCTACGTGGTACTAAGCGTGATGACGTTGAACGTGGCCAAGTACTTG
CTGCTAAAGGTTCAATCAACCCACACACTAAATTTGAGTCAGAAGTATACGTTCTTTCTAAAGATGAAGGCGGCCGTCAT
ACTCCTTTCTTCAAAGGCTACCGTCCACAGTTCTACTTCCGTACAACGGATGTAACTGGTGATATCACACTTCCTGAAGG
TGTTGAAATGGTAATGCCTGGTGATAACGTTCAAATGACGGTTGAGCTAATTGCTCCAATCGCAATGGACGAAGGTCTAC
GCTTCGCAATCCGTGAAGGTGGCCGTACAGTTGGTGCTGGTGTTGTTGCTAAAATCTTCGCTTAA

Upstream 100 bases:

>100_bases
ACACTATTGCGCCAAAGTCAATTGACGCATAAAATAGCAAATTCTGGCGCGCCTTATCATTGTCGGATAGGGTGCCTCAT
AACTAGGAAGGAACACGATC

Downstream 100 bases:

>100_bases
TAGCTAAAGATTTTGTGATAATCGCATAACTAAAAAAGGGAAGCTTAGGCTTCCCTTTTTGTTATTTGGTGTATAGAGAA
TAGGGCGGTTACTTAGAGAG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPFIVVFMNKCDMVDDEELLELVEMEVRELLSE
YDYPGDDLPVIQGSALGALNGEKQWEDKIIELAEALDTYIPLPERAVDMPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIIGIKETTVSTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Sequences:

>Translated_394_residues
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPFIVVFMNKCDMVDDEELLELVEMEVRELLSE
YDYPGDDLPVIQGSALGALNGEKQWEDKIIELAEALDTYIPLPERAVDMPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
DEVEIIGIKETTVSTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRH
TPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
>Mature_393_residues
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERGITISTSHVEYDTPARHYAHVD
CPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLGRQVGIPFIVVFMNKCDMVDDEELLELVEMEVRELLSEY
DYPGDDLPVIQGSALGALNGEKQWEDKIIELAEALDTYIPLPERAVDMPFLLPIEDVFSIQGRGTVVTGRIERGILRVGD
EVEIIGIKETTVSTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSINPHTKFESEVYVLSKDEGGRHT
PFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMTVELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=55.6962025316456, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.5454545454545, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.5454545454545, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=353, Percent_Identity=32.5779036827195, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.2511210762332, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI54607086, Length=343, Percent_Identity=28.8629737609329, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018522, Length=331, Percent_Identity=29.607250755287, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=29.1666666666667, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=29.1666666666667, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=29.5031055900621, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4503507, Length=285, Percent_Identity=27.719298245614, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI53729339, Length=229, Percent_Identity=31.8777292576419, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI53729337, Length=229, Percent_Identity=31.8777292576419, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=27.3809523809524, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.0909090909091, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=83.502538071066, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=83.7150127226463, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789108, Length=325, Percent_Identity=28.9230769230769, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI48994988, Length=250, Percent_Identity=31.2, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788922, Length=339, Percent_Identity=26.5486725663717, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.8771929824561, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.1860465116279, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=331, Percent_Identity=31.4199395770393, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569207, Length=331, Percent_Identity=31.4199395770393, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=430, Percent_Identity=27.6744186046512, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=27.6102088167053, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=318, Percent_Identity=29.559748427673, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=356, Percent_Identity=27.247191011236, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17506081, Length=242, Percent_Identity=29.7520661157025, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17509919, Length=441, Percent_Identity=27.2108843537415, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=151, Percent_Identity=33.7748344370861, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=61.7721518987342, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=28.8636363636364, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=28.8636363636364, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=25, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.7619047619048, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=28.8065843621399, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=56.0411311053985, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=56.0411311053985, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=50.2577319587629, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=28.1318681318681, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=28.1318681318681, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=28.1318681318681, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=28.1318681318681, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=27.7533039647577, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=27.7533039647577, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=29.8013245033113, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=26.605504587156, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.5977011494253, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24647046, Length=376, Percent_Identity=26.5957446808511, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=376, Percent_Identity=26.5957446808511, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28572034, Length=230, Percent_Identity=31.7391304347826, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43177; Mature: 43046

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERG
CCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITISTSHVEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEECCEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPFIVVFMNKCDMVDDEELLELVEMEVRELLSEYDYPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKQWEDKIIELAEALDTYIPLPERAVDMPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIIGIKETTVSTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSIN
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
SKEKFERTKPHVNVGTIGHVDHGKTTLTAAICTTLAKIYGGVAKDFASIDNAPEERERG
CHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
ITISTSHVEYDTPARHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEEECCEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHH
LLGRQVGIPFIVVFMNKCDMVDDEELLELVEMEVRELLSEYDYPGDDLPVIQGSALGALN
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
GEKQWEDKIIELAEALDTYIPLPERAVDMPFLLPIEDVFSIQGRGTVVTGRIERGILRVG
CCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHHHCCCCCCEEEEEHHHCCCEECC
DEVEIIGIKETTVSTCTGVEMFRKLLDEGRAGENVGALLRGTKRDDVERGQVLAAKGSIN
CEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEECCCCC
PHTKFESEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGDITLPEGVEMVMPGDNVQMT
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCEEEECCCCCEEE
VELIAPIAMDEGLRFAIREGGRTVGAGVVAKIFA
EEEHHHHHHCCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA