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The map label for this gene is rfaD [H]

Identifier: 209693872

GI number: 209693872

Start: 310915

End: 311856

Strand: Reverse

Name: rfaD [H]

Synonym: VSAL_I0264

Alternate gene names: 209693872

Gene position: 311856-310915 (Counterclockwise)

Preceding gene: 209693880

Following gene: 209693871

Centisome position: 9.38

GC content: 42.89

Gene sequence:

>942_bases
ATGATCATTGTAACTGGCGGCGCTGGCATGATTGGCAGCAACATTATTAAGTCTTTAAATGACAACGGCTTTAACGATAT
CTTAGTGGTTGATAACCTGAAAGATGGCAAAAAATTCAAGAACCTAGTTGATCTTGATATCACTGATTACATGGATAAAG
AAGATTTCATTACGCAAATCATGGCGGGTGATGACTTTGGTACAATTGATGCTATTTTCCACGAAGGTGCGTGCTCTGCT
ACCACTGAGTGGGATGGCAAATACATGATGATGAACAACTATGAATACTCGAAAGAAGTGCTTCATTTTTGTATCGAACG
TGAGATCCCATTCTTATACGCATCATCTGCTGCGACTTATGGCGATACTGATACCTTCATTGAAGAGCGTGAGTACGAAG
GTGCATTAAACGTTTACGGCTATTCTAAACAACAGTTTGATAACTACGTTCGCCGCCTATGGGCTGATGCTGAAGCGCAC
GGTGAAACGGTTTCTCAAATTACCGGTTTCCGTTACTTCAATGTCTATGGTCCTCGTGAGCAACATAAAGGTTCTATGGC
TTCGGTTGCTTTCCATCTGAACAATCAAATGAATGCCAGCGAAGATCCTAAACTGTTTGAGCGCAGTGATGAATTCAAGC
GTGATTTCGTTTACGTGGGTGATGTGGCTGCGGTTAACTTGTGGTTCTTAGAAAATGGCGTATCAGGTATTTTCAACTGT
GGTACTGGTCGTGCTGAACCGTTCCGCGCTGTTGCGGATGCGGTAATTAAGCACCACGGTAAAGGCGAAGTTCAAAGCAT
TCCGTTCCCTGAGCATTTAAAAGGCGCATATCAAGAGTTTACGCAAGCAGATTTAACTAAGTTGCGAGCTGCGGGTTGTG
ATGTTGAGTTTAAGTCGGTAGCTGATGGTGTTGCTGAGTATATGGCGATTGTTAATAAGTAA

Upstream 100 bases:

>100_bases
GACGACCACTCACTAATACCCTGTCGCAAAATTACCGCTGCCTTTTACTCCTTTCTTTGCTACAATCGAATCAATTATCT
AAATAAATCGTGGTATCAAA

Downstream 100 bases:

>100_bases
AGCTCTGAGAAGTAAAAGCTATACGCTTTGCTAACTAGAAACTAGATCGCTTCGCTTCTATAAGAGCTAGGTTTCAGGTT
TCAGGTTTCAGAAAGAGCGT

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIIVTGGAGMIGSNIIKSLNDNGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGTIDAIFHEGACSA
TTEWDGKYMMMNNYEYSKEVLHFCIEREIPFLYASSAATYGDTDTFIEEREYEGALNVYGYSKQQFDNYVRRLWADAEAH
GETVSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNASEDPKLFERSDEFKRDFVYVGDVAAVNLWFLENGVSGIFNC
GTGRAEPFRAVADAVIKHHGKGEVQSIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAIVNK

Sequences:

>Translated_313_residues
MIIVTGGAGMIGSNIIKSLNDNGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGTIDAIFHEGACSA
TTEWDGKYMMMNNYEYSKEVLHFCIEREIPFLYASSAATYGDTDTFIEEREYEGALNVYGYSKQQFDNYVRRLWADAEAH
GETVSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNASEDPKLFERSDEFKRDFVYVGDVAAVNLWFLENGVSGIFNC
GTGRAEPFRAVADAVIKHHGKGEVQSIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAIVNK
>Mature_313_residues
MIIVTGGAGMIGSNIIKSLNDNGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQIMAGDDFGTIDAIFHEGACSA
TTEWDGKYMMMNNYEYSKEVLHFCIEREIPFLYASSAATYGDTDTFIEEREYEGALNVYGYSKQQFDNYVRRLWADAEAH
GETVSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNASEDPKLFERSDEFKRDFVYVGDVAAVNLWFLENGVSGIFNC
GTGRAEPFRAVADAVIKHHGKGEVQSIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSVADGVAEYMAIVNK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=314, Percent_Identity=73.8853503184713, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 35122; Mature: 35122

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGMIGSNIIKSLNDNGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQI
CEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCHHHHHHHH
MAGDDFGTIDAIFHEGACSATTEWDGKYMMMNNYEYSKEVLHFCIEREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCC
GDTDTFIEEREYEGALNVYGYSKQQFDNYVRRLWADAEAHGETVSQITGFRYFNVYGPRE
CCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCHHHCCHHHHHHHCEEEEEECCCHH
QHKGSMASVAFHLNNQMNASEDPKLFERSDEFKRDFVYVGDVAAVNLWFLENGVSGIFNC
HHCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHCEEEECCHHEEEEEEECCCCCCEEEC
GTGRAEPFRAVADAVIKHHGKGEVQSIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ADGVAEYMAIVNK
HHHHHHHHHHHCC
>Mature Secondary Structure
MIIVTGGAGMIGSNIIKSLNDNGFNDILVVDNLKDGKKFKNLVDLDITDYMDKEDFITQI
CEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHCCHHHHHHHH
MAGDDFGTIDAIFHEGACSATTEWDGKYMMMNNYEYSKEVLHFCIEREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCC
GDTDTFIEEREYEGALNVYGYSKQQFDNYVRRLWADAEAHGETVSQITGFRYFNVYGPRE
CCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCHHHCCHHHHHHHCEEEEEECCCHH
QHKGSMASVAFHLNNQMNASEDPKLFERSDEFKRDFVYVGDVAAVNLWFLENGVSGIFNC
HHCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHCEEEECCHHEEEEEEECCCCCCEEEC
GTGRAEPFRAVADAVIKHHGKGEVQSIPFPEHLKGAYQEFTQADLTKLRAAGCDVEFKSV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ADGVAEYMAIVNK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA