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The map label for this gene is bioH

Identifier: 209693814

GI number: 209693814

Start: 239701

End: 240486

Strand: Reverse

Name: bioH

Synonym: VSAL_I0199

Alternate gene names: 209693814

Gene position: 240486-239701 (Counterclockwise)

Preceding gene: 209693816

Following gene: 209693813

Centisome position: 7.23

GC content: 46.06

Gene sequence:

>786_bases
ATGACAACGTCGCTTTATTGGCAAACGGAAGGTGAAGGTTCCGATCTGGTTCTCATTCATGGATGGGGAATGAACGGGGC
GGTGTGGCAGCCTATTGTTGAGAAGCTAAGTTCACAGTACCGAGTTCATACCGTAGACTTATCAGGTTACGGATACAGTG
CCGAGCTTGGCAGTGCTGATTTTGATGAGATGGTGGCGCAAGTACTCGCTCAGGCACCAGAAAAATCGGCGTGGCTTGGA
TGGTCTCTTGGTGGGTTAATTGCAACGCAAGCCGCATTAACGGCTCCAGATCGTGTGAGTCAATTAATTACCGTAGCAAG
TTCGCCTCGCTTTGCTGCTGAGAAAGGGTGGCGAGGAATAAAGTCTGCCGTGTTGTCCCAATTTACTGAGCAGTTAAAAG
AGGACTTCACCTTAACCGTTGAGCGCTTTATGACGTTGCAAGCAATGGGTAGCCCAAACGCCAAGCAAGACATTAAACAA
GTAAAACGCGCCGTGCTTTCAAGACCAGCACCAAATCCGAGTGCTTTAGCTACAGGTTTAACGATTTTGGCCGATATAGA
CTTACGAGAGAGTCTATCTCAATTAACAATGCCAGTATGCCGAATGTATGGTCGTTTGGATGGATTAGTGCCGATAAAAG
TCGCGCATGATATGGATGCGTTTATCCCTCATTCAACCAAAGTAGTCTTTGAACAAGCCTCGCATGCTCCTTTTATTTCG
CACAGTGATGAATTTATCACTGAGTTACATCGTTTTTTGCAGCCGGAAGATGAGTTTTTAATTTAA

Upstream 100 bases:

>100_bases
ATAATCACAATGGCTAACCCCTAACTTTTGCATTGACCTTCGCAGTAACCTGAGGAACACTGAACCCTCCATTATTTTTT
AACACGAATAGGCAGTGTAG

Downstream 100 bases:

>100_bases
CTTTCTGCTCAGTGTTACGATAAGGAAACGCTTTATGAACTTTTCAATTAACAGTGTTAACGTGCCGTTGATTGCACCCT
CTGTTAATTTGCCAACAGAA

Product: carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTTSLYWQTEGEGSDLVLIHGWGMNGAVWQPIVEKLSSQYRVHTVDLSGYGYSAELGSADFDEMVAQVLAQAPEKSAWLG
WSLGGLIATQAALTAPDRVSQLITVASSPRFAAEKGWRGIKSAVLSQFTEQLKEDFTLTVERFMTLQAMGSPNAKQDIKQ
VKRAVLSRPAPNPSALATGLTILADIDLRESLSQLTMPVCRMYGRLDGLVPIKVAHDMDAFIPHSTKVVFEQASHAPFIS
HSDEFITELHRFLQPEDEFLI

Sequences:

>Translated_261_residues
MTTSLYWQTEGEGSDLVLIHGWGMNGAVWQPIVEKLSSQYRVHTVDLSGYGYSAELGSADFDEMVAQVLAQAPEKSAWLG
WSLGGLIATQAALTAPDRVSQLITVASSPRFAAEKGWRGIKSAVLSQFTEQLKEDFTLTVERFMTLQAMGSPNAKQDIKQ
VKRAVLSRPAPNPSALATGLTILADIDLRESLSQLTMPVCRMYGRLDGLVPIKVAHDMDAFIPHSTKVVFEQASHAPFIS
HSDEFITELHRFLQPEDEFLI
>Mature_260_residues
TTSLYWQTEGEGSDLVLIHGWGMNGAVWQPIVEKLSSQYRVHTVDLSGYGYSAELGSADFDEMVAQVLAQAPEKSAWLGW
SLGGLIATQAALTAPDRVSQLITVASSPRFAAEKGWRGIKSAVLSQFTEQLKEDFTLTVERFMTLQAMGSPNAKQDIKQV
KRAVLSRPAPNPSALATGLTILADIDLRESLSQLTMPVCRMYGRLDGLVPIKVAHDMDAFIPHSTKVVFEQASHAPFISH
SDEFITELHRFLQPEDEFLI

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=245, Percent_Identity=51.4285714285714, Blast_Score=261, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_ALISL (B6EPQ0)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261742.1
- ProteinModelPortal:   B6EPQ0
- MEROPS:   S33.010
- GeneID:   6986370
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0199
- HOGENOM:   HBG748003
- OMA:   VKTMAIM
- ProtClustDB:   CLSK874891
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 28749; Mature: 28618

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: ACT_SITE 82-82 ACT_SITE 207-207 ACT_SITE 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSLYWQTEGEGSDLVLIHGWGMNGAVWQPIVEKLSSQYRVHTVDLSGYGYSAELGSAD
CCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
FDEMVAQVLAQAPEKSAWLGWSLGGLIATQAALTAPDRVSQLITVASSPRFAAEKGWRGI
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
KSAVLSQFTEQLKEDFTLTVERFMTLQAMGSPNAKQDIKQVKRAVLSRPAPNPSALATGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
TILADIDLRESLSQLTMPVCRMYGRLDGLVPIKVAHDMDAFIPHSTKVVFEQASHAPFIS
HHEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCCC
HSDEFITELHRFLQPEDEFLI
CCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTSLYWQTEGEGSDLVLIHGWGMNGAVWQPIVEKLSSQYRVHTVDLSGYGYSAELGSAD
CCEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
FDEMVAQVLAQAPEKSAWLGWSLGGLIATQAALTAPDRVSQLITVASSPRFAAEKGWRGI
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
KSAVLSQFTEQLKEDFTLTVERFMTLQAMGSPNAKQDIKQVKRAVLSRPAPNPSALATGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
TILADIDLRESLSQLTMPVCRMYGRLDGLVPIKVAHDMDAFIPHSTKVVFEQASHAPFIS
HHEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHHHCCCCCCCC
HSDEFITELHRFLQPEDEFLI
CCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA