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The map label for this gene is ilvA [H]

Identifier: 209693682

GI number: 209693682

Start: 55198

End: 56730

Strand: Reverse

Name: ilvA [H]

Synonym: VSAL_I0052

Alternate gene names: 209693682

Gene position: 56730-55198 (Counterclockwise)

Preceding gene: 209693683

Following gene: 209693681

Centisome position: 1.71

GC content: 42.27

Gene sequence:

>1533_bases
ATGACAACACCTTCTTATCAAACTGGCGCAGAATACCTGCGTCAGATCTTACGGGCACCGGTCTATGATGTGGCGACAGT
AACGCAGCTTCAAGACATGCCCCGTTTGTCTGAACGAATTCAAAACCGAGTTCAATTAAAACGAGAAGATCGTCAACCCG
TCCACTCGTTTAAACTTCGCGGCGCTTACAATATGGTGTCGAGCTTGACCGAAGAGCAACAGAAAGCTGGCGTTATCGCA
GCCTCTGCAGGTAATCACGCTCAAGGAATGGCGTTATCAGGCACGAAACTCGGTATTCAAACCACGATAGTCATGCCACG
CACCACCCCTGATATAAAAGTAGAAGCGGTTAAAGGTTTTGGCGGTCACGTTATATTACATGGTCGAAATTTTGATGAAG
CCAAAGCCGAAGCCGAACGATTAGCCACGCTTCATGGCTATACCTTTGTACCTCCATTTGATCACCCATTAGTAATTGCT
GGTCAGGGAACAATTGGTATGGAGATGCTGCAACAAAATGGACATTTAGATTATATATTTGTTCCTGTTGGTGGTGGTGG
CTTAGCCGCGGGAGTTGCCGTTCTCATTAAACAGCTCATGCCAGAGATAAAAGTCATCGCCGTAGAACCAGAAGATTCAG
CTTGTTTAAAAGCCGCATTAGATGCTGGAGAGCCTGTAGTTCTCGATCAAGTGAGTATGTTCGCTGATGGGGTTGCCGTT
AAACGAATTGGTGATGAAACGTTCCGTTTATGCCAACAATATATTGATGGTCATATTACCGTTTCTAGCGATGAGATTTG
TTCCGCAGTAAAAGACATCTTTGAAGACACTAGAGCAATTGCCGAGCCATCAGGTGCACTCGCTTTAGCTGGATTAAAGA
AATTCGCTGACCAGCACCAACTTAAAGACAAGCAACTAAGTACGGTGCTTTCAGGTGCAAATACTAATTTTCATGGGTTA
CGTTACGTATCAGAACGCTGTGAATTAGGCGAAAAACGAGAAGGGTTACTTGCCGTTACTATTCCAGAACGTCAGGGAGC
ATTCTTTGAGTTTTGTAATCTTATTGGTGGAAGGGCGGTTACAGAGTTCAATTATCGTTATCATGACGATAAAAAAGCCA
ATATATTTGTGGGTGTTCGTTTACAAGATGGACAAGAAGAGCTGAATAATATCATTCATGACTTACAACAAGGTGACTAT
CCTGTTGTTGATCTTTCTGATGATGAAATGGCAAAGCTTCATATCCGTTATATGATCGGTGGAAAACCACTAAAACCACT
TAAAGAACGTCTATACAGCTTCGAATTTCCAGAATACCCCGGTGCACTTGTTAAATTTTTAAGTACATTAGGTACACATT
GGAATATAAGCTTATTTAACTACCGTAACCACGGTGCTGATTACGGTCGAGTCTTGTGTGGCTTTGAGTTGAGAGACTCA
GATCTTATTGATTTTTCTAAGCATTTAGAAGAGCTTGGCTATCAATGCAAAGATGAAACTAATAACCCAGCTTATCAATT
TTTTCTCTCATAA

Upstream 100 bases:

>100_bases
CAACGTGAAGTCTCTTTTGCACTAAAAGCTTACGCAATGATGGCGACCAGTGCAGATAAAGGCGCGGTACGTGATAGATC
TAAGTTAGAAGGGTAAATTT

Downstream 100 bases:

>100_bases
CACCATAAGTAATGAGTAAGGTCAGAAAAAAACTGGCCTTATTTTCATAGATTTTTGAAATTATGCTATACAATCAGTTA
AAAAGTGCTATTATTTTTCT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTTPSYQTGAEYLRQILRAPVYDVATVTQLQDMPRLSERIQNRVQLKREDRQPVHSFKLRGAYNMVSSLTEEQQKAGVIA
ASAGNHAQGMALSGTKLGIQTTIVMPRTTPDIKVEAVKGFGGHVILHGRNFDEAKAEAERLATLHGYTFVPPFDHPLVIA
GQGTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAV
KRIGDETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLKDKQLSTVLSGANTNFHGL
RYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYHDDKKANIFVGVRLQDGQEELNNIIHDLQQGDY
PVVDLSDDEMAKLHIRYMIGGKPLKPLKERLYSFEFPEYPGALVKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELRDS
DLIDFSKHLEELGYQCKDETNNPAYQFFLS

Sequences:

>Translated_510_residues
MTTPSYQTGAEYLRQILRAPVYDVATVTQLQDMPRLSERIQNRVQLKREDRQPVHSFKLRGAYNMVSSLTEEQQKAGVIA
ASAGNHAQGMALSGTKLGIQTTIVMPRTTPDIKVEAVKGFGGHVILHGRNFDEAKAEAERLATLHGYTFVPPFDHPLVIA
GQGTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAV
KRIGDETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLKDKQLSTVLSGANTNFHGL
RYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYHDDKKANIFVGVRLQDGQEELNNIIHDLQQGDY
PVVDLSDDEMAKLHIRYMIGGKPLKPLKERLYSFEFPEYPGALVKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELRDS
DLIDFSKHLEELGYQCKDETNNPAYQFFLS
>Mature_509_residues
TTPSYQTGAEYLRQILRAPVYDVATVTQLQDMPRLSERIQNRVQLKREDRQPVHSFKLRGAYNMVSSLTEEQQKAGVIAA
SAGNHAQGMALSGTKLGIQTTIVMPRTTPDIKVEAVKGFGGHVILHGRNFDEAKAEAERLATLHGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDYIFVPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVK
RIGDETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLKDKQLSTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYHDDKKANIFVGVRLQDGQEELNNIIHDLQQGDYP
VVDLSDDEMAKLHIRYMIGGKPLKPLKERLYSFEFPEYPGALVKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELRDSD
LIDFSKHLEELGYQCKDETNNPAYQFFLS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=294, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI19923959, Length=279, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33469958, Length=325, Percent_Identity=27.3846153846154, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1790207, Length=502, Percent_Identity=69.7211155378486, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=295, Percent_Identity=38.3050847457627, Blast_Score=189, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71991565, Length=322, Percent_Identity=34.7826086956522, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=321, Percent_Identity=35.5140186915888, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17508781, Length=288, Percent_Identity=33.6805555555556, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320930, Length=514, Percent_Identity=48.8326848249027, Blast_Score=463, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322631, Length=289, Percent_Identity=37.7162629757785, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319788, Length=268, Percent_Identity=25, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355833, Length=310, Percent_Identity=35.1612903225806, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24645328, Length=251, Percent_Identity=31.0756972111554, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56571; Mature: 56440

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSYQTGAEYLRQILRAPVYDVATVTQLQDMPRLSERIQNRVQLKREDRQPVHSFKLR
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GAYNMVSSLTEEQQKAGVIAASAGNHAQGMALSGTKLGIQTTIVMPRTTPDIKVEAVKGF
HHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECC
GGHVILHGRNFDEAKAEAERLATLHGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIF
CCEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEE
VPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAV
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHH
KRIGDETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFADQHQ
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKDKQLSTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAV
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEE
TEFNYRYHDDKKANIFVGVRLQDGQEELNNIIHDLQQGDYPVVDLSDDEMAKLHIRYMIG
EEECCEECCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEC
GKPLKPLKERLYSFEFPEYPGALVKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELRDS
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEECCC
DLIDFSKHLEELGYQCKDETNNPAYQFFLS
CHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
TTPSYQTGAEYLRQILRAPVYDVATVTQLQDMPRLSERIQNRVQLKREDRQPVHSFKLR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GAYNMVSSLTEEQQKAGVIAASAGNHAQGMALSGTKLGIQTTIVMPRTTPDIKVEAVKGF
HHHHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECC
GGHVILHGRNFDEAKAEAERLATLHGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIF
CCEEEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEE
VPVGGGGLAAGVAVLIKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAV
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHH
KRIGDETFRLCQQYIDGHITVSSDEICSAVKDIFEDTRAIAEPSGALALAGLKKFADQHQ
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKDKQLSTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAV
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEE
TEFNYRYHDDKKANIFVGVRLQDGQEELNNIIHDLQQGDYPVVDLSDDEMAKLHIRYMIG
EEECCEECCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEC
GKPLKPLKERLYSFEFPEYPGALVKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELRDS
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEEECCC
DLIDFSKHLEELGYQCKDETNNPAYQFFLS
CHHHHHHHHHHCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]