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The map label for this gene is nylA [H]

Identifier: 209552186

GI number: 209552186

Start: 295010

End: 296518

Strand: Reverse

Name: nylA [H]

Synonym: Rleg2_6344

Alternate gene names: 209552186

Gene position: 296518-295010 (Counterclockwise)

Preceding gene: 209552187

Following gene: 209552185

Centisome position: 96.04

GC content: 59.44

Gene sequence:

>1509_bases
ATGCCCTCTTTCGACTCCGATTACGGCAAGCGGGACGGCCTCGAACTGGCCGACTTACTTTATTGCCGTGAGATATCGCC
CAAGGAATTGATGGAAAGTGCGATAGCGGCCGCGGAGGCAGTGAACCCAGCTCTTAATGCGTTTTGCTTTCCGCGTCATG
AAGCCGCGCTCGTGGAAGCGGAGAAGGCAACGCTTCAAGGCACGTTCGGCGCTCTACCATTCGCATTCAAGGATTCCGGA
CTTGCCGCACAAGGTCACGGCGGAAGTATTGGATCACGGCTTTTCTCAAAGTCGATGTCGCACACGGATTCGACTCTAGC
TGCGCGGTTCCGGAAGGACGGCTTCATCAGCTTCGGCCGGACAACCGTTCCCGAAATGTGTATGGCGCCGACGACCGAGG
CCAGGCAGAACGGCGGTGCTACCCGGAATCCTTGGGATCACAGCCGCTCGGCAGGCGGCTCCTCCGGCGGGGCGGCAGTT
GCGGTTGCGACTGGCGTTGTCCCGATTGCACACGGAAGTGATGGAGGCGGATCAATACGAATTCCCGCTGCCTGTTGTGG
CCTATATGGGTTGAAGACCTCACGTGGACTGGTGCCACACGGCCCGAACAAAGGAGAAGGCTGGGGAGGCCTCGCAGTCC
ATGGGATCCTGACCCGTTCGGTTTACGATAGCGCCGCCGCACTCAACGGAATCGCGGGAATGGAGCCCGGCGCGCCTTAT
GCGTCGCCGGAAAGGCCGGATTCTTTCCTGGAATCTCTAGAGCTGCCATTCGATCGCGCCCTTAGGATCGCCAAATGGAC
GGTCGGCTGGGACGGCATCCCCATTGCTCCCGACTGTCTGGCGGCCCTGGAAAAGGCAGAACACGCGCTCGTCGCACTGG
GCCACGAGGTCGTGGACGTTTATCCGCCGGAGCTCGATTACACCGCATTCGTTGAAGCTTTGATTGACGTCCTTTGCACG
AATGCAGCGATGTCCGTCAGCGATTTCCTGACATCCAATCCGGTGGAGAATTGGCGGGATCTCCTCGAGCCTGCGATCCA
AGATGCCTGCGCGATCGGAAACTCTTTGCCTGCGATGCGCTACGTGGCAGCGATCAGTACCTTCCATCGAGTGGGTAGGG
CGCTCGACTCTTACATGGCGTCATACGATTTCGTTATTTCGCCAACCCTCACGCAGCCGCCGCTACCGCTTGCAGAGCTG
TCGATGGACACGGATTTCCGTTCGTTCAGGCGCAAGGCGGCCAGGTATACGATGTTCCTGGCACTGTTGAATGCTTCCGG
TCAGCCCGCCGCCAACCTTCCTTTATTCAAAAGCAGTTCTGGACTGCCGATCGGCGTTCAACTCATCGGCCGCTTCGGCC
AAGACGCAGAAATCCTCAAACTATCCGCGCAGCTCGAGTGTGAGACGGGCTGGGCCGATCTTCAATTCGTCCGTTCGCAC
GCCGCTCAGGCCGGACACGCGCAAGACCTCAACGGAAGGAAGACGGCTCCTGCTTCGCAGCGAGCCTGA

Upstream 100 bases:

>100_bases
ATGAACCGGCCCTTGTAGCGGACAAATGAGCGGCGGATCCGATCCAAACCCGCCGCTCGCAATGCTCAATTCGAGCGGTT
GAAAATCATGGGACTTCGCA

Downstream 100 bases:

>100_bases
ATTTACGCCAGTCCGATTTGAACCAAAAAAGGCGAACCAATGAAAAAAGGGAACGGTGTGGTTACGGTATTCAAGAAGCA
CGACACCGAAGCATCGCCCG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPSFDSDYGKRDGLELADLLYCREISPKELMESAIAAAEAVNPALNAFCFPRHEAALVEAEKATLQGTFGALPFAFKDSG
LAAQGHGGSIGSRLFSKSMSHTDSTLAARFRKDGFISFGRTTVPEMCMAPTTEARQNGGATRNPWDHSRSAGGSSGGAAV
AVATGVVPIAHGSDGGGSIRIPAACCGLYGLKTSRGLVPHGPNKGEGWGGLAVHGILTRSVYDSAAALNGIAGMEPGAPY
ASPERPDSFLESLELPFDRALRIAKWTVGWDGIPIAPDCLAALEKAEHALVALGHEVVDVYPPELDYTAFVEALIDVLCT
NAAMSVSDFLTSNPVENWRDLLEPAIQDACAIGNSLPAMRYVAAISTFHRVGRALDSYMASYDFVISPTLTQPPLPLAEL
SMDTDFRSFRRKAARYTMFLALLNASGQPAANLPLFKSSSGLPIGVQLIGRFGQDAEILKLSAQLECETGWADLQFVRSH
AAQAGHAQDLNGRKTAPASQRA

Sequences:

>Translated_502_residues
MPSFDSDYGKRDGLELADLLYCREISPKELMESAIAAAEAVNPALNAFCFPRHEAALVEAEKATLQGTFGALPFAFKDSG
LAAQGHGGSIGSRLFSKSMSHTDSTLAARFRKDGFISFGRTTVPEMCMAPTTEARQNGGATRNPWDHSRSAGGSSGGAAV
AVATGVVPIAHGSDGGGSIRIPAACCGLYGLKTSRGLVPHGPNKGEGWGGLAVHGILTRSVYDSAAALNGIAGMEPGAPY
ASPERPDSFLESLELPFDRALRIAKWTVGWDGIPIAPDCLAALEKAEHALVALGHEVVDVYPPELDYTAFVEALIDVLCT
NAAMSVSDFLTSNPVENWRDLLEPAIQDACAIGNSLPAMRYVAAISTFHRVGRALDSYMASYDFVISPTLTQPPLPLAEL
SMDTDFRSFRRKAARYTMFLALLNASGQPAANLPLFKSSSGLPIGVQLIGRFGQDAEILKLSAQLECETGWADLQFVRSH
AAQAGHAQDLNGRKTAPASQRA
>Mature_501_residues
PSFDSDYGKRDGLELADLLYCREISPKELMESAIAAAEAVNPALNAFCFPRHEAALVEAEKATLQGTFGALPFAFKDSGL
AAQGHGGSIGSRLFSKSMSHTDSTLAARFRKDGFISFGRTTVPEMCMAPTTEARQNGGATRNPWDHSRSAGGSSGGAAVA
VATGVVPIAHGSDGGGSIRIPAACCGLYGLKTSRGLVPHGPNKGEGWGGLAVHGILTRSVYDSAAALNGIAGMEPGAPYA
SPERPDSFLESLELPFDRALRIAKWTVGWDGIPIAPDCLAALEKAEHALVALGHEVVDVYPPELDYTAFVEALIDVLCTN
AAMSVSDFLTSNPVENWRDLLEPAIQDACAIGNSLPAMRYVAAISTFHRVGRALDSYMASYDFVISPTLTQPPLPLAELS
MDTDFRSFRRKAARYTMFLALLNASGQPAANLPLFKSSSGLPIGVQLIGRFGQDAEILKLSAQLECETGWADLQFVRSHA
AQAGHAQDLNGRKTAPASQRA

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=25.0972762645914, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI195972892, Length=208, Percent_Identity=32.6923076923077, Blast_Score=94, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=271, Percent_Identity=30.6273062730627, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=338, Percent_Identity=27.2189349112426, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=194, Percent_Identity=32.9896907216495, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=245, Percent_Identity=29.3877551020408, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=245, Percent_Identity=29.3877551020408, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=312, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=146, Percent_Identity=33.5616438356164, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=27.8455284552846, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=266, Percent_Identity=27.8195488721804, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648435, Length=266, Percent_Identity=27.8195488721804, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648437, Length=266, Percent_Identity=27.8195488721804, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652985, Length=506, Percent_Identity=25.4940711462451, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922090, Length=506, Percent_Identity=25.4940711462451, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24652981, Length=506, Percent_Identity=25.4940711462451, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24652983, Length=506, Percent_Identity=25.4940711462451, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644968, Length=204, Percent_Identity=34.3137254901961, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648113, Length=517, Percent_Identity=27.0793036750484, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356731, Length=202, Percent_Identity=31.1881188118812, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648441, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648439, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53141; Mature: 53009

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFDSDYGKRDGLELADLLYCREISPKELMESAIAAAEAVNPALNAFCFPRHEAALVEA
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEECCCCHHHHHHH
EKATLQGTFGALPFAFKDSGLAAQGHGGSIGSRLFSKSMSHTDSTLAARFRKDGFISFGR
HHHHHCCCCCCCCEEECCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCC
TTVPEMCMAPTTEARQNGGATRNPWDHSRSAGGSSGGAAVAVATGVVPIAHGSDGGGSIR
CCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEE
IPAACCGLYGLKTSRGLVPHGPNKGEGWGGLAVHGILTRSVYDSAAALNGIAGMEPGAPY
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ASPERPDSFLESLELPFDRALRIAKWTVGWDGIPIAPDCLAALEKAEHALVALGHEVVDV
CCCCCCHHHHHHHCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCC
YPPELDYTAFVEALIDVLCTNAAMSVSDFLTSNPVENWRDLLEPAIQDACAIGNSLPAMR
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
YVAAISTFHRVGRALDSYMASYDFVISPTLTQPPLPLAELSMDTDFRSFRRKAARYTMFL
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
ALLNASGQPAANLPLFKSSSGLPIGVQLIGRFGQDAEILKLSAQLECETGWADLQFVRSH
HHHCCCCCCCCCCCCEECCCCCEEHHHHHHCCCCCHHEEEEEEEEEECCCHHHHHHHHHH
AAQAGHAQDLNGRKTAPASQRA
HHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSFDSDYGKRDGLELADLLYCREISPKELMESAIAAAEAVNPALNAFCFPRHEAALVEA
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHEECCCCHHHHHHH
EKATLQGTFGALPFAFKDSGLAAQGHGGSIGSRLFSKSMSHTDSTLAARFRKDGFISFGR
HHHHHCCCCCCCCEEECCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCC
TTVPEMCMAPTTEARQNGGATRNPWDHSRSAGGSSGGAAVAVATGVVPIAHGSDGGGSIR
CCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCEEE
IPAACCGLYGLKTSRGLVPHGPNKGEGWGGLAVHGILTRSVYDSAAALNGIAGMEPGAPY
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ASPERPDSFLESLELPFDRALRIAKWTVGWDGIPIAPDCLAALEKAEHALVALGHEVVDV
CCCCCCHHHHHHHCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCC
YPPELDYTAFVEALIDVLCTNAAMSVSDFLTSNPVENWRDLLEPAIQDACAIGNSLPAMR
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
YVAAISTFHRVGRALDSYMASYDFVISPTLTQPPLPLAELSMDTDFRSFRRKAARYTMFL
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
ALLNASGQPAANLPLFKSSSGLPIGVQLIGRFGQDAEILKLSAQLECETGWADLQFVRSH
HHHCCCCCCCCCCCCEECCCCCEEHHHHHHCCCCCHHEEEEEEEEEECCCHHHHHHHHHH
AAQAGHAQDLNGRKTAPASQRA
HHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]