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The map label for this gene is dgoA [C]

Identifier: 209552119

GI number: 209552119

Start: 214552

End: 215655

Strand: Reverse

Name: dgoA [C]

Synonym: Rleg2_6270

Alternate gene names: 209552119

Gene position: 215655-214552 (Counterclockwise)

Preceding gene: 209552120

Following gene: 209552118

Centisome position: 69.85

GC content: 56.88

Gene sequence:

>1104_bases
ATGAAAATCCGATCAATTTCGCTTTACGAACTGGAACTTCCCCTAACGGCTGTGCAATATCTTTCCCGCGGCCGGACATT
CGAGAGATTTCTATCCACCTTCGTGAGGATTGATACCGATGAGGGTCTTACCGGCTGGGGAGAGGTTTGCCCGTGGGGCT
CATCTTACCTTCCTGCGTTTCCCGGCGGCGTCAGAACCGCATTGGCGGAGATATCCCCTCATTTGATTGGACAGGACCCC
TGCCGACCGGACCAGCTGAACCGCTTGATGGATACGGTGCTGACAGGACATATCTATGCAAAAGCCGGGATTGACTACGC
CCTGTGGGATCTCGTCGGGCAGGCGGCAGGTCGGCCAATCTATGACGTGATCGGCGGGAAAGAGGACAACCCCGTGCCGA
TCTCGGCCGCTATTCATCATGGAACGCCCGACGCGATGATGCGGGAGATGGAAAAATTTAGGCAGCGAGGTGCCAAACGC
TTCTCTGTCAAGGTTGGCCAGGGCGTGAACACCGACATCGAGGTCGTTAAACGTTTCACAGAAAGCAGGCGGGAAGACGA
GCTGATGATCTTCGACGCCAATTGCGGATGGAGTCCGTGGGAAGCCATCAGGGTGATGAACGCCACGGCTGATCTCGACA
CCACTTTCGAGCAGCCATGTGCCACGTACGAACAGTGCCTGATCGTCCGCCAGCAGACACAGCAGCCGCTTTGCCTGGAC
GAGTGCCTAGTCGAACTCAAGGACTTTGTCAGGGCGGTATCTGACCGGGCTTGCCAGATCGTGAATATCAAGATCGCCAG
GGTGGGCGGTATCACCAAAGCACGCACTATTCGTGACTTTCTCCTGACCTACGACATTTCGATGCTGACAATGTGCATGG
CGGGGACGACAATAAACGACACGATGTGCGCGCATTTCGGCCAGACGGTTCCGGCCAACCGCTTTGTCGGAACCTGGAGC
TGCCAAGACTACGTAACGGTAGACCCCGCTCCTGGTCGCGGCGCCCGTAGCGAAGGTGGTTTGATGCCTCCTCCGAACCT
GCCTGGGCTAGGGGTTGCACCAGATCTGGAAGTGCTACGCACACCGTTTGCCGTGTTCCAATAG

Upstream 100 bases:

>100_bases
GACGGCGGTTACTCCGCGCGGTGATGCGACGCTGGCAACAACTGCGTCCTGAACAATTTCAAACCTTAGGCTCAAGTCCG
AATCGCGCATGAGGAACACC

Downstream 100 bases:

>100_bases
ACACGAGAGGCAAGTAGAGGGCCTCCCCCGGTTCAGCACACGGGGCTTTAAGCAATTCACTTCTCGCAGCACGACCAGAC
ACCCCGGCGTTTGCCGGGCC

Product: mandelate racemase/muconate lactonizing protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKIRSISLYELELPLTAVQYLSRGRTFERFLSTFVRIDTDEGLTGWGEVCPWGSSYLPAFPGGVRTALAEISPHLIGQDP
CRPDQLNRLMDTVLTGHIYAKAGIDYALWDLVGQAAGRPIYDVIGGKEDNPVPISAAIHHGTPDAMMREMEKFRQRGAKR
FSVKVGQGVNTDIEVVKRFTESRREDELMIFDANCGWSPWEAIRVMNATADLDTTFEQPCATYEQCLIVRQQTQQPLCLD
ECLVELKDFVRAVSDRACQIVNIKIARVGGITKARTIRDFLLTYDISMLTMCMAGTTINDTMCAHFGQTVPANRFVGTWS
CQDYVTVDPAPGRGARSEGGLMPPPNLPGLGVAPDLEVLRTPFAVFQ

Sequences:

>Translated_367_residues
MKIRSISLYELELPLTAVQYLSRGRTFERFLSTFVRIDTDEGLTGWGEVCPWGSSYLPAFPGGVRTALAEISPHLIGQDP
CRPDQLNRLMDTVLTGHIYAKAGIDYALWDLVGQAAGRPIYDVIGGKEDNPVPISAAIHHGTPDAMMREMEKFRQRGAKR
FSVKVGQGVNTDIEVVKRFTESRREDELMIFDANCGWSPWEAIRVMNATADLDTTFEQPCATYEQCLIVRQQTQQPLCLD
ECLVELKDFVRAVSDRACQIVNIKIARVGGITKARTIRDFLLTYDISMLTMCMAGTTINDTMCAHFGQTVPANRFVGTWS
CQDYVTVDPAPGRGARSEGGLMPPPNLPGLGVAPDLEVLRTPFAVFQ
>Mature_367_residues
MKIRSISLYELELPLTAVQYLSRGRTFERFLSTFVRIDTDEGLTGWGEVCPWGSSYLPAFPGGVRTALAEISPHLIGQDP
CRPDQLNRLMDTVLTGHIYAKAGIDYALWDLVGQAAGRPIYDVIGGKEDNPVPISAAIHHGTPDAMMREMEKFRQRGAKR
FSVKVGQGVNTDIEVVKRFTESRREDELMIFDANCGWSPWEAIRVMNATADLDTTFEQPCATYEQCLIVRQQTQQPLCLD
ECLVELKDFVRAVSDRACQIVNIKIARVGGITKARTIRDFLLTYDISMLTMCMAGTTINDTMCAHFGQTVPANRFVGTWS
CQDYVTVDPAPGRGARSEGGLMPPPNLPGLGVAPDLEVLRTPFAVFQ

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=350, Percent_Identity=24.5714285714286, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081876, Length=299, Percent_Identity=24.7491638795987, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 40730; Mature: 40730

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSISLYELELPLTAVQYLSRGRTFERFLSTFVRIDTDEGLTGWGEVCPWGSSYLPAF
CCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCC
PGGVRTALAEISPHLIGQDPCRPDQLNRLMDTVLTGHIYAKAGIDYALWDLVGQAAGRPI
CCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCH
YDVIGGKEDNPVPISAAIHHGTPDAMMREMEKFRQRGAKRFSVKVGQGVNTDIEVVKRFT
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
ESRREDELMIFDANCGWSPWEAIRVMNATADLDTTFEQPCATYEQCLIVRQQTQQPLCLD
HHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
ECLVELKDFVRAVSDRACQIVNIKIARVGGITKARTIRDFLLTYDISMLTMCMAGTTIND
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TMCAHFGQTVPANRFVGTWSCQDYVTVDPAPGRGARSEGGLMPPPNLPGLGVAPDLEVLR
HHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
TPFAVFQ
CCHHHCC
>Mature Secondary Structure
MKIRSISLYELELPLTAVQYLSRGRTFERFLSTFVRIDTDEGLTGWGEVCPWGSSYLPAF
CCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCC
PGGVRTALAEISPHLIGQDPCRPDQLNRLMDTVLTGHIYAKAGIDYALWDLVGQAAGRPI
CCHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCH
YDVIGGKEDNPVPISAAIHHGTPDAMMREMEKFRQRGAKRFSVKVGQGVNTDIEVVKRFT
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
ESRREDELMIFDANCGWSPWEAIRVMNATADLDTTFEQPCATYEQCLIVRQQTQQPLCLD
HHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
ECLVELKDFVRAVSDRACQIVNIKIARVGGITKARTIRDFLLTYDISMLTMCMAGTTIND
HHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
TMCAHFGQTVPANRFVGTWSCQDYVTVDPAPGRGARSEGGLMPPPNLPGLGVAPDLEVLR
HHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
TPFAVFQ
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]