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The map label for this gene is citE [C]

Identifier: 209551971

GI number: 209551971

Start: 35672

End: 36478

Strand: Reverse

Name: citE [C]

Synonym: Rleg2_6108

Alternate gene names: 209551971

Gene position: 36478-35672 (Counterclockwise)

Preceding gene: 209551972

Following gene: 209551970

Centisome position: 11.81

GC content: 59.23

Gene sequence:

>807_bases
ATGTCCATCACCACAATGCTATTTGTTCCCGCAGACCGTCCCGAACGCTTCGCAAAGGCGGCGGCCAGCGGGGCGGATGC
CGTAATCCTTGACCTTGAGGATGCTGTAGCCCCCGAGGGCAAGGCGGCGGCGCGCGAAAACGCCGCGCGATCAAGTACCA
ATGCAAGGACGTTTATTCGGATCAATCCAGCTAGCAGCGACGATTTTCCTGAGGACGTCTCGCTACTAGCCCGTGCCGGC
TTTCGAAACGTCATGCTGTCCAAAGCGGAAACGCCTGGGGAGCTAGAGCGCGTCGTGAAAGAACTGGGCGAGACGGTCAC
GATCGTCCCATTGATCGAGACAGCCGTAGGGCTATCGAACGCCCGTGAACTCGCCGCTCACCGGGCCGTACCTTTCCTGG
CGTTTGGCAGTCTCGATTTCGCTCTCGATCTGGGCTGCGAACACACGCATGAGGCGCTTAGGTTTGCTCGTCAGACACTC
GTCTACTCTTCTCGTCTAGCGCGAAAGGCCGCGCCGATCGACGGTGTGACAGCCGCTATCGATGATGCCGTGCTTATAAA
CGCGGATGCTAGCGAGGCCCGTAGCTTGGGCTTCGCGGGAAAACTCCTCATCCATCCGAAGCAGATTGCGCCCGTGAGGC
AAGCGTTTCAGCCGTCCGAAACCGAGATGCGCTGGGCAAAGCAGGTCCTCGATGCTGTTGCACAGAACGGGACGAAGGCC
GTAAAAGTGAATGGGCAAATGGTCGATCGCCCGGTTATGAGCAGGGCGGAGCGAATACTCTCGCTCAGCGCGTCGTATGA
TGGCTGA

Upstream 100 bases:

>100_bases
AGCACCAGCTCTCGAATAGAGAAGTGCTGCGTTCCGTCGCCTAGCGCCCGATCTCCCATTTCACGTCAGAAGGTTCCGCA
TTGGAGAGAATGAGCTCACT

Downstream 100 bases:

>100_bases
CCCGCGATCGAAAGGATGGTTGTCAAGTCGCAAACGCCCCATTAGTGCATTTCGAAATTAGTCTGCACACGCCTCAGCCG
CGCAGGGGTGATATTGTAGG

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSITTMLFVPADRPERFAKAAASGADAVILDLEDAVAPEGKAAARENAARSSTNARTFIRINPASSDDFPEDVSLLARAG
FRNVMLSKAETPGELERVVKELGETVTIVPLIETAVGLSNARELAAHRAVPFLAFGSLDFALDLGCEHTHEALRFARQTL
VYSSRLARKAAPIDGVTAAIDDAVLINADASEARSLGFAGKLLIHPKQIAPVRQAFQPSETEMRWAKQVLDAVAQNGTKA
VKVNGQMVDRPVMSRAERILSLSASYDG

Sequences:

>Translated_268_residues
MSITTMLFVPADRPERFAKAAASGADAVILDLEDAVAPEGKAAARENAARSSTNARTFIRINPASSDDFPEDVSLLARAG
FRNVMLSKAETPGELERVVKELGETVTIVPLIETAVGLSNARELAAHRAVPFLAFGSLDFALDLGCEHTHEALRFARQTL
VYSSRLARKAAPIDGVTAAIDDAVLINADASEARSLGFAGKLLIHPKQIAPVRQAFQPSETEMRWAKQVLDAVAQNGTKA
VKVNGQMVDRPVMSRAERILSLSASYDG
>Mature_267_residues
SITTMLFVPADRPERFAKAAASGADAVILDLEDAVAPEGKAAARENAARSSTNARTFIRINPASSDDFPEDVSLLARAGF
RNVMLSKAETPGELERVVKELGETVTIVPLIETAVGLSNARELAAHRAVPFLAFGSLDFALDLGCEHTHEALRFARQTLV
YSSRLARKAAPIDGVTAAIDDAVLINADASEARSLGFAGKLLIHPKQIAPVRQAFQPSETEMRWAKQVLDAVAQNGTKAV
KVNGQMVDRPVMSRAERILSLSASYDG

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=290, Percent_Identity=28.2758620689655, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081764, Length=278, Percent_Identity=29.8561151079137, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17557458, Length=303, Percent_Identity=27.3927392739274, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28694; Mature: 28563

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITTMLFVPADRPERFAKAAASGADAVILDLEDAVAPEGKAAARENAARSSTNARTFIR
CCEEEEEEEECCCHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHHHHHHCCCCCCEEEEE
INPASSDDFPEDVSLLARAGFRNVMLSKAETPGELERVVKELGETVTIVPLIETAVGLSN
EECCCCCCCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEHHHHHHCHHH
ARELAAHRAVPFLAFGSLDFALDLGCEHTHEALRFARQTLVYSSRLARKAAPIDGVTAAI
HHHHHHHHCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DDAVLINADASEARSLGFAGKLLIHPKQIAPVRQAFQPSETEMRWAKQVLDAVAQNGTKA
CCEEEEECCHHHHHHCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEE
VKVNGQMVDRPVMSRAERILSLSASYDG
EEECCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SITTMLFVPADRPERFAKAAASGADAVILDLEDAVAPEGKAAARENAARSSTNARTFIR
CEEEEEEEECCCHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHHHHHHCCCCCCEEEEE
INPASSDDFPEDVSLLARAGFRNVMLSKAETPGELERVVKELGETVTIVPLIETAVGLSN
EECCCCCCCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEHHHHHHCHHH
ARELAAHRAVPFLAFGSLDFALDLGCEHTHEALRFARQTLVYSSRLARKAAPIDGVTAAI
HHHHHHHHCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DDAVLINADASEARSLGFAGKLLIHPKQIAPVRQAFQPSETEMRWAKQVLDAVAQNGTKA
CCEEEEECCHHHHHHCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEE
VKVNGQMVDRPVMSRAERILSLSASYDG
EEECCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]