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The map label for this gene is spsJ [H]

Identifier: 209550525

GI number: 209550525

Start: 3005477

End: 3006433

Strand: Direct

Name: spsJ [H]

Synonym: Rleg2_2948

Alternate gene names: 209550525

Gene position: 3005477-3006433 (Clockwise)

Preceding gene: 209550524

Following gene: 209550526

Centisome position: 66.23

GC content: 57.37

Gene sequence:

>957_bases
ATGAAAAGCGTATTGATTTCTGGCGGGGCTGGATTTATCGGCTCGCATTTGTGCGACAGGCTTTTACTTAGAACCGACGT
CGAAAAGCTGGTTGTTGTTGATAATCTCTGGACGGGACTTTTCGACAATATCGCCCACATCCGGGATCCCCGCTTCCACT
TCGTGAAGTCGGACGTCGAGACGTTGCGCAGTTCTGAAAAATTCGATGAAATCTATCATCTTGCCTCACCGGCATCGCCG
CCATGGTACATGAAGGAGCCGAAGAGGACGATTTCCGCCAATCTTCTCGGGGCATTCCGGCTGCTGGAGCTTTTGAAGAA
GGGTGGACGTTTCGGCTTTACCTCGACCTCCGAAGTCTATGGCGACCCGCTGGTATCGCCGCAGCCGGAATCCTATAAGG
GCCAGGTGGACTGCACCGGACCGCGCTCGTCCTACGACGAGAGCAAGAGGTGCACGGAATCGCTGCTGTTCGAAATGCAG
CGCACCCAAGGGCTCGACCTCAAGGTCGTCCGTCCCTTCAACATTTATGGTCCTCGCACACGGTCCGATGACGGCCGCGC
CGTCTCCAACTTCATCACCCAGGCTCTGGCGGGCCGGCCGATCACCGTATTCGGCGACGGCAAGCAGTCGCGCAGCTGGG
GTTATGTCGATGACGTCGTCGACGGTTTCGCCCGCTATTTCTGGATCAATGAAACCGACTACAAGGGACCTCTGAACGTC
GGCAATGATCGCGAGATTTCGGTGCTCGAAGTCGCACAATATGTGTCCAAACTGGTCGGCGGCGTGCCGATCGTCTTCGA
GCCCTCACCGCCGCAGGACCCGACAAACAGGCGCCCGGACCTGACCAACGCCAATTACGTCATGCCCGAGTGGTCGTGCA
AGATCAGCTACGAGCAGGGCGTGGCCATGACGCTTGACTGGTTCAGAGACAAGATAAAGGTGGGCGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
TAATAAGGGAGTATTTTCTAGAATACTTCTAAAGAATTGCCATGCCTTAAAGGTGATGCTAATAAACGCTTACCTACTCA
ACGTCATAAGGCGCAATGCA

Downstream 100 bases:

>100_bases
ACCATCACGGCTTTTGGGCTTCGCGTCTTTGCTGGCGCGGCATATGCTCGCGGTGCGATTTGAGAGAGTGGTAATGCGTG
AACCAATACCGTCCGATCTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKSVLISGGAGFIGSHLCDRLLLRTDVEKLVVVDNLWTGLFDNIAHIRDPRFHFVKSDVETLRSSEKFDEIYHLASPASP
PWYMKEPKRTISANLLGAFRLLELLKKGGRFGFTSTSEVYGDPLVSPQPESYKGQVDCTGPRSSYDESKRCTESLLFEMQ
RTQGLDLKVVRPFNIYGPRTRSDDGRAVSNFITQALAGRPITVFGDGKQSRSWGYVDDVVDGFARYFWINETDYKGPLNV
GNDREISVLEVAQYVSKLVGGVPIVFEPSPPQDPTNRRPDLTNANYVMPEWSCKISYEQGVAMTLDWFRDKIKVGAAE

Sequences:

>Translated_318_residues
MKSVLISGGAGFIGSHLCDRLLLRTDVEKLVVVDNLWTGLFDNIAHIRDPRFHFVKSDVETLRSSEKFDEIYHLASPASP
PWYMKEPKRTISANLLGAFRLLELLKKGGRFGFTSTSEVYGDPLVSPQPESYKGQVDCTGPRSSYDESKRCTESLLFEMQ
RTQGLDLKVVRPFNIYGPRTRSDDGRAVSNFITQALAGRPITVFGDGKQSRSWGYVDDVVDGFARYFWINETDYKGPLNV
GNDREISVLEVAQYVSKLVGGVPIVFEPSPPQDPTNRRPDLTNANYVMPEWSCKISYEQGVAMTLDWFRDKIKVGAAE
>Mature_318_residues
MKSVLISGGAGFIGSHLCDRLLLRTDVEKLVVVDNLWTGLFDNIAHIRDPRFHFVKSDVETLRSSEKFDEIYHLASPASP
PWYMKEPKRTISANLLGAFRLLELLKKGGRFGFTSTSEVYGDPLVSPQPESYKGQVDCTGPRSSYDESKRCTESLLFEMQ
RTQGLDLKVVRPFNIYGPRTRSDDGRAVSNFITQALAGRPITVFGDGKQSRSWGYVDDVVDGFARYFWINETDYKGPLNV
GNDREISVLEVAQYVSKLVGGVPIVFEPSPPQDPTNRRPDLTNANYVMPEWSCKISYEQGVAMTLDWFRDKIKVGAAE

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=318, Percent_Identity=42.7672955974843, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=25.0764525993884, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=28.1159420289855, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788589, Length=337, Percent_Identity=27.893175074184, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=25.3521126760563, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786974, Length=299, Percent_Identity=27.0903010033445, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788365, Length=328, Percent_Identity=25.3048780487805, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=43.8095238095238, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=28.7833827893175, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115532424, Length=236, Percent_Identity=30.0847457627119, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=246, Percent_Identity=27.2357723577236, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356223, Length=314, Percent_Identity=43.312101910828, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 35750; Mature: 35750

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVLISGGAGFIGSHLCDRLLLRTDVEKLVVVDNLWTGLFDNIAHIRDPRFHFVKSDVE
CCCEEECCCCCHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLRSSEKFDEIYHLASPASPPWYMKEPKRTISANLLGAFRLLELLKKGGRFGFTSTSEVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
GDPLVSPQPESYKGQVDCTGPRSSYDESKRCTESLLFEMQRTQGLDLKVVRPFNIYGPRT
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCC
RSDDGRAVSNFITQALAGRPITVFGDGKQSRSWGYVDDVVDGFARYFWINETDYKGPLNV
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCC
GNDREISVLEVAQYVSKLVGGVPIVFEPSPPQDPTNRRPDLTNANYVMPEWSCKISYEQG
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCC
VAMTLDWFRDKIKVGAAE
CEEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSVLISGGAGFIGSHLCDRLLLRTDVEKLVVVDNLWTGLFDNIAHIRDPRFHFVKSDVE
CCCEEECCCCCHHHHHHHHHHHHHHCHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLRSSEKFDEIYHLASPASPPWYMKEPKRTISANLLGAFRLLELLKKGGRFGFTSTSEVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
GDPLVSPQPESYKGQVDCTGPRSSYDESKRCTESLLFEMQRTQGLDLKVVRPFNIYGPRT
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCC
RSDDGRAVSNFITQALAGRPITVFGDGKQSRSWGYVDDVVDGFARYFWINETDYKGPLNV
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCC
GNDREISVLEVAQYVSKLVGGVPIVFEPSPPQDPTNRRPDLTNANYVMPEWSCKISYEQG
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCC
VAMTLDWFRDKIKVGAAE
CEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]