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The map label for this gene is yafC [H]

Identifier: 209550308

GI number: 209550308

Start: 2775666

End: 2776565

Strand: Direct

Name: yafC [H]

Synonym: Rleg2_2728

Alternate gene names: 209550308

Gene position: 2775666-2776565 (Clockwise)

Preceding gene: 209550306

Following gene: 209550309

Centisome position: 61.17

GC content: 64.11

Gene sequence:

>900_bases
ATGAGTACCGAACCCAGCTGGGATTTCTACCGCGCTTTCCTGACCGTGCTTCAGCAAGGGTCGCTTTCGGCCGCCGCCCG
CGAGTTGGGGCTGACGCAGCCGACCATAGGCCGCCATGTCGACGCATTGGAAACGGCAATCGGCGCCGAACTCTTCACCC
GATCCCCGAACGGGCTGCTGCCGACCGATGCCGCATTGGCGCTGAAGCCCTATGCCGAGACGCTGGCGGCAACCACCGCC
GCGCTTTTGCGCACCGCATCCGGTGAGCGCGAGCGCGTCGCCGGAACCGTCAGGGTCAGCGCCAGCGAGGTCATCGCCGT
CGAAGTACTGCCTGGGATTTTCGGACCGCTGCAGGACGCCTACCCCGAACTCCAGATCGAACTCTCGGCCTCCGATGCGA
TTGAGGATCTGGTGAACCGCGAGGCCGATATCGCCGTGCGCATGGCCGAGCCGCAGCAGGGTGCGCTCGTCGTGCGCCGC
ATCGGCGATATCCCGCTTGGTTTCCACGCCCATCGCCGCTACCTCGAACGACACGGCATTCCGCAAACCCTGGCTGACCT
CGCAGACCACCGGCTCATCGGCTTCGACCGCCAGACGGCCTATGTCCGCCTGGTAACAAAACGCTATGAAGTGCCGGGCA
TCGACTTCTCCTATAGGACCGACAGCAATCTTGCCCAGCTTTCGGCGATCCGCGCCGGCGTCGGCATCGGTCTCTGCCAG
ATTGGGCTCGCACGTAATAATCCCGATCTGGTGCACCTACTGCCCGAGGCGTTCAAGATCCCGCTCGCCACGTGGGTGGC
GATGCATGAGAGCCTGAAGACCTCGCCGCGCTGCCGCGCCACCTTCGACGCGCTGGTCAAAGGGCTTCAGGACTATCACC
GATATTCGACCGGCACATAA

Upstream 100 bases:

>100_bases
GCTCATGACAATCATCTCCTTCGCTTGAATGGAAGGACTATGCCACCGGCCCTAAATAAACAAAATTGACGAAATAAATG
CATCTGCTATACAAAAATAT

Downstream 100 bases:

>100_bases
CCTCGAATCGGGAATCGATTTTCGGAGAGAATCATGCGCAAGTTTCAACGCAAGAGAGCATCCGAAAGCTGGCCTGTCGG
ATGCGCGGCGCTGTAGTGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSTEPSWDFYRAFLTVLQQGSLSAAARELGLTQPTIGRHVDALETAIGAELFTRSPNGLLPTDAALALKPYAETLAATTA
ALLRTASGERERVAGTVRVSASEVIAVEVLPGIFGPLQDAYPELQIELSASDAIEDLVNREADIAVRMAEPQQGALVVRR
IGDIPLGFHAHRRYLERHGIPQTLADLADHRLIGFDRQTAYVRLVTKRYEVPGIDFSYRTDSNLAQLSAIRAGVGIGLCQ
IGLARNNPDLVHLLPEAFKIPLATWVAMHESLKTSPRCRATFDALVKGLQDYHRYSTGT

Sequences:

>Translated_299_residues
MSTEPSWDFYRAFLTVLQQGSLSAAARELGLTQPTIGRHVDALETAIGAELFTRSPNGLLPTDAALALKPYAETLAATTA
ALLRTASGERERVAGTVRVSASEVIAVEVLPGIFGPLQDAYPELQIELSASDAIEDLVNREADIAVRMAEPQQGALVVRR
IGDIPLGFHAHRRYLERHGIPQTLADLADHRLIGFDRQTAYVRLVTKRYEVPGIDFSYRTDSNLAQLSAIRAGVGIGLCQ
IGLARNNPDLVHLLPEAFKIPLATWVAMHESLKTSPRCRATFDALVKGLQDYHRYSTGT
>Mature_298_residues
STEPSWDFYRAFLTVLQQGSLSAAARELGLTQPTIGRHVDALETAIGAELFTRSPNGLLPTDAALALKPYAETLAATTAA
LLRTASGERERVAGTVRVSASEVIAVEVLPGIFGPLQDAYPELQIELSASDAIEDLVNREADIAVRMAEPQQGALVVRRI
GDIPLGFHAHRRYLERHGIPQTLADLADHRLIGFDRQTAYVRLVTKRYEVPGIDFSYRTDSNLAQLSAIRAGVGIGLCQI
GLARNNPDLVHLLPEAFKIPLATWVAMHESLKTSPRCRATFDALVKGLQDYHRYSTGT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=193, Percent_Identity=27.979274611399, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789639, Length=184, Percent_Identity=27.1739130434783, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787589, Length=185, Percent_Identity=25.9459459459459, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=23, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787128, Length=231, Percent_Identity=25.1082251082251, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32622; Mature: 32490

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEPSWDFYRAFLTVLQQGSLSAAARELGLTQPTIGRHVDALETAIGAELFTRSPNGLL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PTDAALALKPYAETLAATTAALLRTASGERERVAGTVRVSASEVIAVEVLPGIFGPLQDA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEEEHHHHHHHHHHHHC
YPELQIELSASDAIEDLVNREADIAVRMAEPQQGALVVRRIGDIPLGFHAHRRYLERHGI
CCCEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCC
PQTLADLADHRLIGFDRQTAYVRLVTKRYEVPGIDFSYRTDSNLAQLSAIRAGVGIGLCQ
HHHHHHHHCCCEECCCCCHHHHEEHHHHCCCCCCCEECCCCCHHHHHHHHHHCCCHHHHH
IGLARNNPDLVHLLPEAFKIPLATWVAMHESLKTSPRCRATFDALVKGLQDYHRYSTGT
EECCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STEPSWDFYRAFLTVLQQGSLSAAARELGLTQPTIGRHVDALETAIGAELFTRSPNGLL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PTDAALALKPYAETLAATTAALLRTASGERERVAGTVRVSASEVIAVEVLPGIFGPLQDA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEEEHHHHHHHHHHHHC
YPELQIELSASDAIEDLVNREADIAVRMAEPQQGALVVRRIGDIPLGFHAHRRYLERHGI
CCCEEEEECHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCC
PQTLADLADHRLIGFDRQTAYVRLVTKRYEVPGIDFSYRTDSNLAQLSAIRAGVGIGLCQ
HHHHHHHHCCCEECCCCCHHHHEEHHHHCCCCCCCEECCCCCHHHHHHHHHHCCCHHHHH
IGLARNNPDLVHLLPEAFKIPLATWVAMHESLKTSPRCRATFDALVKGLQDYHRYSTGT
EECCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]