Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 209550305

GI number: 209550305

Start: 2772551

End: 2773675

Strand: Direct

Name: nemA [H]

Synonym: Rleg2_2725

Alternate gene names: 209550305

Gene position: 2772551-2773675 (Clockwise)

Preceding gene: 209550304

Following gene: 209550306

Centisome position: 61.1

GC content: 63.02

Gene sequence:

>1125_bases
ATGGCCAAGCTTTTCGAACCCACCAAGGTTGGCGACATTACAGTCAAGAACCGCATCGTCATGGCGCCGCTCACCCGCAA
CCGCTCGCCGGGCGCCATCCCGAACGACCTCAATGTCGAATATTATCGCCAGCGCGCCACGGCAGGCCTGATCATTACCG
AAGCGACCGCGATCACCCATCAGGGCCAGGGTTACGCCAACGTCCCCGGTCTCTACAGCAAAGAGGCTCTCGACGGCTGG
AAGCGCGTCACCGAAGCCGTTCACGCGGCCGGCGGCAAGATCGTCGTCCAGATGTGGCATGTCGGCCGCATCTCGCACAC
GACGCTGCAGCCGGATGGCGGCAAACCCGTCTCCTCGACCGACCGGGTCGCCAAGGCCAAGACCTACCTGGTCAATGGCG
ACGGCACCGGCAGCTTTGCCGACACCTCCGAGCCGCGCGCCCTAGAAACCGCTGAAATCCCCGGTATCATCGAAGATTAC
CGCAAGGCCGCGCGTGCGGCGATCGACGCCGGCTTCGACGGCGTCGAGATCCATGGCGCCAACGGCTACCTGCTCGACCA
GTTCATCCGCGACGGCGTCAACGACCGCACCGATCAATATGGCGGCTCGATCGAAAACCGCGCGCGCCTGACCTTCGATG
TCGTCGATGCCGTCGTCAGCGAAATCGGCGCAGGCCGCACCGCGATCCGCATCTCGCCGGTGACACCGTCGGGCGAAAGC
TACGATTCCAATCCGCAGGCGACTTTCACCCATGTCGTCGAGGGGCTGGCCAGATACGATCTCGCCTATATCCATGTGAT
CGAAGGCCAGACCGGCGGTGATCGCGACTACCGCCAGGGCGACAATCCTTCCTTCGATTACAAGGCGTTGCGCCAGGCTT
ATGAAACTGCCGGCGGCAAGGCCGACTGGATGGTCAATAACGGTTATGACCGCGATCTGGCGATCGACGCCGTCGAAAGC
GGCCGCGTCGACCTCGTCGCCTTCGGCAAGCCTTTCATCGCCAATCCCGATCTCGTCGAACGGCTGGCGAAGAACCTGCC
GCTCAACACGCCTGACCAGTCGACCTTCTATGGCGGCACCGCCAAGGGCTACGTCGACTATCCCGCTCTCGAAAAGGTCG
CCTGA

Upstream 100 bases:

>100_bases
CTGATCGGCCTCGGCGCGACGGCGCTCACCTATCTCTCCGCCAGGGGCCGAGCCGCCCTCGCCCCTGCCGAGTGATCCTC
CCGCAAAAGAAAGGACCATC

Downstream 100 bases:

>100_bases
ACCTTTCGAATACACATTGCGAATCCCGCCTGAAGGCGGGATTTTCATTTCCCGCGCATCTTTGTAGGGTGTGCCTATGT
TCGCTGCCCATCTTGATCGC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MAKLFEPTKVGDITVKNRIVMAPLTRNRSPGAIPNDLNVEYYRQRATAGLIITEATAITHQGQGYANVPGLYSKEALDGW
KRVTEAVHAAGGKIVVQMWHVGRISHTTLQPDGGKPVSSTDRVAKAKTYLVNGDGTGSFADTSEPRALETAEIPGIIEDY
RKAARAAIDAGFDGVEIHGANGYLLDQFIRDGVNDRTDQYGGSIENRARLTFDVVDAVVSEIGAGRTAIRISPVTPSGES
YDSNPQATFTHVVEGLARYDLAYIHVIEGQTGGDRDYRQGDNPSFDYKALRQAYETAGGKADWMVNNGYDRDLAIDAVES
GRVDLVAFGKPFIANPDLVERLAKNLPLNTPDQSTFYGGTAKGYVDYPALEKVA

Sequences:

>Translated_374_residues
MAKLFEPTKVGDITVKNRIVMAPLTRNRSPGAIPNDLNVEYYRQRATAGLIITEATAITHQGQGYANVPGLYSKEALDGW
KRVTEAVHAAGGKIVVQMWHVGRISHTTLQPDGGKPVSSTDRVAKAKTYLVNGDGTGSFADTSEPRALETAEIPGIIEDY
RKAARAAIDAGFDGVEIHGANGYLLDQFIRDGVNDRTDQYGGSIENRARLTFDVVDAVVSEIGAGRTAIRISPVTPSGES
YDSNPQATFTHVVEGLARYDLAYIHVIEGQTGGDRDYRQGDNPSFDYKALRQAYETAGGKADWMVNNGYDRDLAIDAVES
GRVDLVAFGKPFIANPDLVERLAKNLPLNTPDQSTFYGGTAKGYVDYPALEKVA
>Mature_373_residues
AKLFEPTKVGDITVKNRIVMAPLTRNRSPGAIPNDLNVEYYRQRATAGLIITEATAITHQGQGYANVPGLYSKEALDGWK
RVTEAVHAAGGKIVVQMWHVGRISHTTLQPDGGKPVSSTDRVAKAKTYLVNGDGTGSFADTSEPRALETAEIPGIIEDYR
KAARAAIDAGFDGVEIHGANGYLLDQFIRDGVNDRTDQYGGSIENRARLTFDVVDAVVSEIGAGRTAIRISPVTPSGESY
DSNPQATFTHVVEGLARYDLAYIHVIEGQTGGDRDYRQGDNPSFDYKALRQAYETAGGKADWMVNNGYDRDLAIDAVESG
RVDLVAFGKPFIANPDLVERLAKNLPLNTPDQSTFYGGTAKGYVDYPALEKVA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=371, Percent_Identity=47.1698113207547, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789463, Length=348, Percent_Identity=27.2988505747126, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17559802, Length=273, Percent_Identity=29.3040293040293, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=29.6416938110749, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=135, Percent_Identity=38.5185185185185, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=269, Percent_Identity=26.3940520446097, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=260, Percent_Identity=25.7692307692308, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=139, Percent_Identity=35.2517985611511, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=226, Percent_Identity=28.7610619469027, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=391, Percent_Identity=38.8746803069054, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=38.560411311054, Blast_Score=224, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40472; Mature: 40341

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLFEPTKVGDITVKNRIVMAPLTRNRSPGAIPNDLNVEYYRQRATAGLIITEATAITH
CCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEEE
QGQGYANVPGLYSKEALDGWKRVTEAVHAAGGKIVVQMWHVGRISHTTLQPDGGKPVSST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCEECCCCCCCCCCH
DRVAKAKTYLVNGDGTGSFADTSEPRALETAEIPGIIEDYRKAARAAIDAGFDGVEIHGA
HHHHEEEEEEEECCCCCCCCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLDQFIRDGVNDRTDQYGGSIENRARLTFDVVDAVVSEIGAGRTAIRISPVTPSGES
CCHHHHHHHHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
YDSNPQATFTHVVEGLARYDLAYIHVIEGQTGGDRDYRQGDNPSFDYKALRQAYETAGGK
CCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
ADWMVNNGYDRDLAIDAVESGRVDLVAFGKPFIANPDLVERLAKNLPLNTPDQSTFYGGT
EEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AKGYVDYPALEKVA
CCCCCCCCHHHHCC
>Mature Secondary Structure 
AKLFEPTKVGDITVKNRIVMAPLTRNRSPGAIPNDLNVEYYRQRATAGLIITEATAITH
CCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEEE
QGQGYANVPGLYSKEALDGWKRVTEAVHAAGGKIVVQMWHVGRISHTTLQPDGGKPVSST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEECCEECCCCCCCCCCH
DRVAKAKTYLVNGDGTGSFADTSEPRALETAEIPGIIEDYRKAARAAIDAGFDGVEIHGA
HHHHEEEEEEEECCCCCCCCCCCCCCCEEHHCCCCHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLDQFIRDGVNDRTDQYGGSIENRARLTFDVVDAVVSEIGAGRTAIRISPVTPSGES
CCHHHHHHHHCCCCCCHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
YDSNPQATFTHVVEGLARYDLAYIHVIEGQTGGDRDYRQGDNPSFDYKALRQAYETAGGK
CCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
ADWMVNNGYDRDLAIDAVESGRVDLVAFGKPFIANPDLVERLAKNLPLNTPDQSTFYGGT
EEEEEECCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AKGYVDYPALEKVA
CCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]