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The map label for this gene is yafC [H]

Identifier: 209550302

GI number: 209550302

Start: 2769491

End: 2770402

Strand: Direct

Name: yafC [H]

Synonym: Rleg2_2722

Alternate gene names: 209550302

Gene position: 2769491-2770402 (Clockwise)

Preceding gene: 209550301

Following gene: 209550303

Centisome position: 61.03

GC content: 60.2

Gene sequence:

>912_bases
ATGAGGGGTGGGGGAATGCCATTGGATTGGGATAAGCTGCGTATTTTTCACGCAGCTGCCGAAGCGGGTTCGTTCACCCA
TGCGGCGGATAAACTCCATTTGTCCCAGTCCGCCATCAGCCGCCAGGTGAGCGCGCTGGAGCAGGATGTCGGCACCAAGC
TGTTTCACCGCCATGCGCGCGGCCTGATCCTGACGGAACAGGGCGAGCTGCTGTACCGCACCGCCCATGACGTGCTGCTG
AAGCTCGAAACCGTGAAGATGCAGTTGACGGAAACCAACGAAACACCGTCCGGCAAGCTGCGCGTCACCACCACAGTCGG
CCTCGGCCAGGGCTGGCTGACCGACAAGATCCAGGAATTCCTGCAGCTTTATCCCGATGTGCAGATCCAGCTGATCCTCG
ACAATGAGGAAGTGGATGTGAACATGCGCCATGCCGACTGCGCGATCCGCCTGCGCCAGCCGCAGCAATCCGACCTCATC
CAGCGCAAGCTCTTCACCGTGCACATGCACGTCTATGCCGCTCCCTCTTACATCAACCGCCATGGCGAGCCGCAGAAGAT
CGAGGATCTCGACAATCACCGCATCATCACCTTCGGCGAGCCGGCGCCGAGCTACCTGCTCGACGTCAACTGGCTTGAGG
TCGCCGGCCGCTCGTCGGACAACAAGCGCATCCCGCATCTGCAGATCAACAGCCAGACCTCGATCAAGCGCGCCGCCCTG
CTCGGCATCGGCGTCGCCTGCCTGCCGGATTACATCGTCGGCCGCGATCCCGGGCTGATTCAGCTGGCGATCAATGCCGA
CGTTCCCTCCTTCGACACCTATTTCTGCTATCCCGATGAGATCAAGAACGCCGCCAAGCTGAAGGCCTTCCGTGATTTCA
TCGTCAGCAAGGCCAGAAACTGGAATTTCTAA

Upstream 100 bases:

>100_bases
GCAATCACTGCCGCCGGTCTTGGATGCATGGCAGCGCTTGAAGCCGAGCGCTATCTGACGGGTCACATGCCCGTCGCCGT
AGCCGCGGAGTAAGATCGGC

Downstream 100 bases:

>100_bases
AACATCATGCCGAAAAGTGTGAGCGGTTGTCGGACGACATCATGCTCACCTGCTCTGATTAGATCCGGATGATTTTCGGC
CGGATCGGCCTTAGATCATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRGGGMPLDWDKLRIFHAAAEAGSFTHAADKLHLSQSAISRQVSALEQDVGTKLFHRHARGLILTEQGELLYRTAHDVLL
KLETVKMQLTETNETPSGKLRVTTTVGLGQGWLTDKIQEFLQLYPDVQIQLILDNEEVDVNMRHADCAIRLRQPQQSDLI
QRKLFTVHMHVYAAPSYINRHGEPQKIEDLDNHRIITFGEPAPSYLLDVNWLEVAGRSSDNKRIPHLQINSQTSIKRAAL
LGIGVACLPDYIVGRDPGLIQLAINADVPSFDTYFCYPDEIKNAAKLKAFRDFIVSKARNWNF

Sequences:

>Translated_303_residues
MRGGGMPLDWDKLRIFHAAAEAGSFTHAADKLHLSQSAISRQVSALEQDVGTKLFHRHARGLILTEQGELLYRTAHDVLL
KLETVKMQLTETNETPSGKLRVTTTVGLGQGWLTDKIQEFLQLYPDVQIQLILDNEEVDVNMRHADCAIRLRQPQQSDLI
QRKLFTVHMHVYAAPSYINRHGEPQKIEDLDNHRIITFGEPAPSYLLDVNWLEVAGRSSDNKRIPHLQINSQTSIKRAAL
LGIGVACLPDYIVGRDPGLIQLAINADVPSFDTYFCYPDEIKNAAKLKAFRDFIVSKARNWNF
>Mature_303_residues
MRGGGMPLDWDKLRIFHAAAEAGSFTHAADKLHLSQSAISRQVSALEQDVGTKLFHRHARGLILTEQGELLYRTAHDVLL
KLETVKMQLTETNETPSGKLRVTTTVGLGQGWLTDKIQEFLQLYPDVQIQLILDNEEVDVNMRHADCAIRLRQPQQSDLI
QRKLFTVHMHVYAAPSYINRHGEPQKIEDLDNHRIITFGEPAPSYLLDVNWLEVAGRSSDNKRIPHLQINSQTSIKRAAL
LGIGVACLPDYIVGRDPGLIQLAINADVPSFDTYFCYPDEIKNAAKLKAFRDFIVSKARNWNF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=31.4189189189189, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=27.6666666666667, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789639, Length=240, Percent_Identity=30.8333333333333, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=27, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=28.6764705882353, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081978, Length=180, Percent_Identity=28.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=23.5494880546075, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1789173, Length=191, Percent_Identity=28.2722513089005, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788706, Length=181, Percent_Identity=27.0718232044199, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=28.4584980237154, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=32.8571428571429, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=23.469387755102, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=120, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=258, Percent_Identity=25.1937984496124, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI2367136, Length=285, Percent_Identity=21.7543859649123, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=24.896265560166, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=310, Percent_Identity=25.1612903225806, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788297, Length=125, Percent_Identity=27.2, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787879, Length=127, Percent_Identity=29.9212598425197, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790783, Length=253, Percent_Identity=27.2727272727273, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1788626, Length=193, Percent_Identity=31.0880829015544, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34231; Mature: 34231

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGGMPLDWDKLRIFHAAAEAGSFTHAADKLHLSQSAISRQVSALEQDVGTKLFHRHAR
CCCCCCCCCHHHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLILTEQGELLYRTAHDVLLKLETVKMQLTETNETPSGKLRVTTTVGLGQGWLTDKIQEF
CEEEECCCCEEEEEHHHHHEEEEEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHH
LQLYPDVQIQLILDNEEVDVNMRHADCAIRLRQPQQSDLIQRKLFTVHMHVYAAPSYINR
HHHCCCCEEEEEECCCCEEEEEEECCEEEEECCCCHHHHHHHHHEEEEEEEEECCHHHHC
HGEPQKIEDLDNHRIITFGEPAPSYLLDVNWLEVAGRSSDNKRIPHLQINSQTSIKRAAL
CCCCCHHHCCCCCEEEEECCCCCCEEEECCHHEEECCCCCCCCCCEEEECCCCHHHHHHH
LGIGVACLPDYIVGRDPGLIQLAINADVPSFDTYFCYPDEIKNAAKLKAFRDFIVSKARN
HHCCHHHHCHHHCCCCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCC
WNF
CCC
>Mature Secondary Structure
MRGGGMPLDWDKLRIFHAAAEAGSFTHAADKLHLSQSAISRQVSALEQDVGTKLFHRHAR
CCCCCCCCCHHHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLILTEQGELLYRTAHDVLLKLETVKMQLTETNETPSGKLRVTTTVGLGQGWLTDKIQEF
CEEEECCCCEEEEEHHHHHEEEEEEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHH
LQLYPDVQIQLILDNEEVDVNMRHADCAIRLRQPQQSDLIQRKLFTVHMHVYAAPSYINR
HHHCCCCEEEEEECCCCEEEEEEECCEEEEECCCCHHHHHHHHHEEEEEEEEECCHHHHC
HGEPQKIEDLDNHRIITFGEPAPSYLLDVNWLEVAGRSSDNKRIPHLQINSQTSIKRAAL
CCCCCHHHCCCCCEEEEECCCCCCEEEECCHHEEECCCCCCCCCCEEEECCCCHHHHHHH
LGIGVACLPDYIVGRDPGLIQLAINADVPSFDTYFCYPDEIKNAAKLKAFRDFIVSKARN
HHCCHHHHCHHHCCCCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCC
WNF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]