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The map label for this gene is rhtB [H]

Identifier: 209550190

GI number: 209550190

Start: 2652656

End: 2653291

Strand: Direct

Name: rhtB [H]

Synonym: Rleg2_2610

Alternate gene names: 209550190

Gene position: 2652656-2653291 (Clockwise)

Preceding gene: 209550189

Following gene: 209550191

Centisome position: 58.45

GC content: 61.01

Gene sequence:

>636_bases
ATGTCACTTGAAGCTTGGCTCGCTTTTGCGGCCGCATCCGCCATCATGCTGGCCATACCGGGACCGACGATACTGCTCGT
CGTTTCCTATGCGCTCGGTCATGGACGCAGGACTGCGTTTGCGACGGTGAGCGGCGTGGCGCTTGGTGACTTCACCGCGA
TGACGGCGTCTCTCTTCGGTCTCGGCGCCGTCCTCGCCGCCTCCGCCACCCTCTTTACCGTCTTGAAGTGGATCGGCGGC
GCCTATCTGATCTGGCTGGGAATCAAGCTCTGGCGGGCTCCTGTCATCGGCGAACCGGTTGCCGACAACGACAATCTGCC
GGAGGAAAAATCGCTTAAGATCTTCCTCCACGCCTATGTCGTCACCGCCCTTAATCCGAAGAGCATTATCTTCTTCGTCG
CCTTCGTGCCGCAGTTCCTCAATCCGGCCCTGCCCTTCGTTGGTCAGATGGCGATCATGGAAGCGACTTTCCTCGTGCTG
GCGATCCTCAACGCTTCCGCTTATGCGTTTCTCGCCCATACCGCGCGCGGACTGATTCGCAAGGCGAGCGTTCAGCGGGC
TGTGAACCGTACGGGCGGGACCCTGCTGATCGCGGCAGGCGCCGTAACCGCCGGGTATCGCCGTATTGCAGCTTAG

Upstream 100 bases:

>100_bases
CGAAGTCATGGACAATATAGGCCAGAAATACAAAGAAGCGTGATTGACGGATGGTTAATATGATGTCTTTGCTTGTTCCT
GAGGAATAGGAGGAGACACC

Downstream 100 bases:

>100_bases
AAATAATCCGTGGTTGCCGGAATGCCACGAATAGGTTAATGGGGTCGTCGGATTTAAGCTTTTTAGTAACTTTTATAACG
CTGCCGGGGCGGCACGATTT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSLEAWLAFAAASAIMLAIPGPTILLVVSYALGHGRRTAFATVSGVALGDFTAMTASLFGLGAVLAASATLFTVLKWIGG
AYLIWLGIKLWRAPVIGEPVADNDNLPEEKSLKIFLHAYVVTALNPKSIIFFVAFVPQFLNPALPFVGQMAIMEATFLVL
AILNASAYAFLAHTARGLIRKASVQRAVNRTGGTLLIAAGAVTAGYRRIAA

Sequences:

>Translated_211_residues
MSLEAWLAFAAASAIMLAIPGPTILLVVSYALGHGRRTAFATVSGVALGDFTAMTASLFGLGAVLAASATLFTVLKWIGG
AYLIWLGIKLWRAPVIGEPVADNDNLPEEKSLKIFLHAYVVTALNPKSIIFFVAFVPQFLNPALPFVGQMAIMEATFLVL
AILNASAYAFLAHTARGLIRKASVQRAVNRTGGTLLIAAGAVTAGYRRIAA
>Mature_210_residues
SLEAWLAFAAASAIMLAIPGPTILLVVSYALGHGRRTAFATVSGVALGDFTAMTASLFGLGAVLAASATLFTVLKWIGGA
YLIWLGIKLWRAPVIGEPVADNDNLPEEKSLKIFLHAYVVTALNPKSIIFFVAFVPQFLNPALPFVGQMAIMEATFLVLA
ILNASAYAFLAHTARGLIRKASVQRAVNRTGGTLLIAAGAVTAGYRRIAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=209, Percent_Identity=34.9282296650718, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788099, Length=195, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786522, Length=127, Percent_Identity=30.7086614173228, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22218; Mature: 22086

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEAWLAFAAASAIMLAIPGPTILLVVSYALGHGRRTAFATVSGVALGDFTAMTASLFG
CCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCHHHHEECCCHHHHHHHHHHHHHH
LGAVLAASATLFTVLKWIGGAYLIWLGIKLWRAPVIGEPVADNDNLPEEKSLKIFLHAYV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHEEHH
VTALNPKSIIFFVAFVPQFLNPALPFVGQMAIMEATFLVLAILNASAYAFLAHTARGLIR
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KASVQRAVNRTGGTLLIAAGAVTAGYRRIAA
HHHHHHHHHCCCCEEEEEECHHHHHHHHHCC
>Mature Secondary Structure 
SLEAWLAFAAASAIMLAIPGPTILLVVSYALGHGRRTAFATVSGVALGDFTAMTASLFG
CHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCHHHHEECCCHHHHHHHHHHHHHH
LGAVLAASATLFTVLKWIGGAYLIWLGIKLWRAPVIGEPVADNDNLPEEKSLKIFLHAYV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHEEHH
VTALNPKSIIFFVAFVPQFLNPALPFVGQMAIMEATFLVLAILNASAYAFLAHTARGLIR
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KASVQRAVNRTGGTLLIAAGAVTAGYRRIAA
HHHHHHHHHCCCCEEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]