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The map label for this gene is pfp [H]

Identifier: 209550189

GI number: 209550189

Start: 2651387

End: 2652598

Strand: Direct

Name: pfp [H]

Synonym: Rleg2_2609

Alternate gene names: 209550189

Gene position: 2651387-2652598 (Clockwise)

Preceding gene: 209550171

Following gene: 209550190

Centisome position: 58.43

GC content: 63.04

Gene sequence:

>1212_bases
ATGGCCAAGCAGAAAGTTGCAATGCTGACCGCCGGAGGCCTGGCGCCCTGTCTTTCCTCCGCCGTCGGCGGCCTCATCGA
GCGCTACAGCGACATCGCCCCCGATCTCGATCTCGTCGCCTACAAGTCCGGCTACCAGGGCGTATTGCTCGGCGATAGCA
TCGAGATCACTCGGGAGATGCGCGAAAAGGCGCCACTGCTGCATCGTTATGGCGGCTCGCCGATCGGCAACAGCCGCGTC
AAGCTCACCAATGCCGCCGACTGCGTCAAGCGCGGCTTGGTCAAGGAAGGCGACAACCCGCTGCGGGTTGCCGCCGAACG
CCTCGCCAGTGACGGTATCACCATTCTCCACACGATCGGCGGCGACGACACCAACACTACCGCTGCCGACCTTGCCGCCT
ATCTCGCCGCCAACGGCTACAACCTGACCGTCGTCGGCCTGCCGAAGACCGTCGACAACGACGTCGTGCCGATCCGTCAG
TCGCTCGGCGCCTGGACGGCCGCCGAAGTCGGCGCGCATTTCTTCGACAATGTCGGCAACGAACAGACGGCCGCGCCCCG
CACCCTCGTCATCCACGAGGTCATGGGCCGCCATTGCGGCTGGCTGACCGCCGCGACCGCCCGCGCCTATCTCCAGCGCA
CCAGCCGCAACCAGTATGTCGACGGCCTGATGATGGACGCGCATCTGAAAAGCATCGACGCCGTCTACCTGCCTGAAATG
GCCTTCGACCTCGACGCCGAGGCCGCCCGCCTGAAGGACAGCATGGACCGCAATGGCCACGCCACGGTCTTCGTCTCTGA
AGGCGCCTGCCTTGATGCCATCGTCGCGGAGCGCGAAGCCGCCGGCGAAACCGTCAAGCGCGACGCCTTCGGCCATGTGA
AGATCGACACGATCAATGTCGGCGCCTGGTTCCAGAAACAGTTCGCCAGCCTGTTGAACGCCGAACGCTCCCTCGTGCAG
AAATCGGGCTACTTCGCCCGCTCGGCGCCTGCCAACAGCGACGACCTCAGGCTGATCCAGAGCATGGTCGATCTTGCCGT
TGAAAGCGCGCTCAACAAAGTCTCCGGCGTCACCGGCCACGATGAAGCGCAGAATGGTAAATTGCGCACTATAGAATTCC
CGCGCATCAAGGGCGGCAAGGCTTTTGACCTGTCGACGGCATGGTTTGCCGAAGTCATGGACAATATAGGCCAGAAATAC
AAAGAAGCGTGA

Upstream 100 bases:

>100_bases
GACATCCATGAAAACAATGCGCTAAGGCGCGTCGCTTGAATCAAATTCGAGGCGACGCCTGTCGCTGAAGCCGCCGCACC
GAAACGCCATAGGAGGGATC

Downstream 100 bases:

>100_bases
TTGACGGATGGTTAATATGATGTCTTTGCTTGTTCCTGAGGAATAGGAGGAGACACCATGTCACTTGAAGCTTGGCTCGC
TTTTGCGGCCGCATCCGCCA

Product: pyrophosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPDLDLVAYKSGYQGVLLGDSIEITREMREKAPLLHRYGGSPIGNSRV
KLTNAADCVKRGLVKEGDNPLRVAAERLASDGITILHTIGGDDTNTTAADLAAYLAANGYNLTVVGLPKTVDNDVVPIRQ
SLGAWTAAEVGAHFFDNVGNEQTAAPRTLVIHEVMGRHCGWLTAATARAYLQRTSRNQYVDGLMMDAHLKSIDAVYLPEM
AFDLDAEAARLKDSMDRNGHATVFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGAWFQKQFASLLNAERSLVQ
KSGYFARSAPANSDDLRLIQSMVDLAVESALNKVSGVTGHDEAQNGKLRTIEFPRIKGGKAFDLSTAWFAEVMDNIGQKY
KEA

Sequences:

>Translated_403_residues
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPDLDLVAYKSGYQGVLLGDSIEITREMREKAPLLHRYGGSPIGNSRV
KLTNAADCVKRGLVKEGDNPLRVAAERLASDGITILHTIGGDDTNTTAADLAAYLAANGYNLTVVGLPKTVDNDVVPIRQ
SLGAWTAAEVGAHFFDNVGNEQTAAPRTLVIHEVMGRHCGWLTAATARAYLQRTSRNQYVDGLMMDAHLKSIDAVYLPEM
AFDLDAEAARLKDSMDRNGHATVFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGAWFQKQFASLLNAERSLVQ
KSGYFARSAPANSDDLRLIQSMVDLAVESALNKVSGVTGHDEAQNGKLRTIEFPRIKGGKAFDLSTAWFAEVMDNIGQKY
KEA
>Mature_402_residues
AKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPDLDLVAYKSGYQGVLLGDSIEITREMREKAPLLHRYGGSPIGNSRVK
LTNAADCVKRGLVKEGDNPLRVAAERLASDGITILHTIGGDDTNTTAADLAAYLAANGYNLTVVGLPKTVDNDVVPIRQS
LGAWTAAEVGAHFFDNVGNEQTAAPRTLVIHEVMGRHCGWLTAATARAYLQRTSRNQYVDGLMMDAHLKSIDAVYLPEMA
FDLDAEAARLKDSMDRNGHATVFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINVGAWFQKQFASLLNAERSLVQK
SGYFARSAPANSDDLRLIQSMVDLAVESALNKVSGVTGHDEAQNGKLRTIEFPRIKGGKAFDLSTAWFAEVMDNIGQKYK
EA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family. PFP subfamily [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=287, Percent_Identity=27.5261324041812, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI266453748, Length=287, Percent_Identity=27.5261324041812, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4505749, Length=287, Percent_Identity=27.5261324041812, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI266453619, Length=285, Percent_Identity=27.719298245614, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790350, Length=295, Percent_Identity=26.1016949152542, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321679, Length=295, Percent_Identity=28.4745762711864, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022953
- InterPro:   IPR000023
- InterPro:   IPR011405 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43343; Mature: 43211

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPDLDLVAYKSGYQGVLLGDSIEITREM
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHH
REKAPLLHRYGGSPIGNSRVKLTNAADCVKRGLVKEGDNPLRVAAERLASDGITILHTIG
HHHCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEC
GDDTNTTAADLAAYLAANGYNLTVVGLPKTVDNDVVPIRQSLGAWTAAEVGAHFFDNVGN
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
EQTAAPRTLVIHEVMGRHCGWLTAATARAYLQRTSRNQYVDGLMMDAHLKSIDAVYLPEM
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCHH
AFDLDAEAARLKDSMDRNGHATVFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINV
HHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEC
GAWFQKQFASLLNAERSLVQKSGYFARSAPANSDDLRLIQSMVDLAVESALNKVSGVTGH
CHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEAQNGKLRTIEFPRIKGGKAFDLSTAWFAEVMDNIGQKYKEA
CCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPDLDLVAYKSGYQGVLLGDSIEITREM
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHH
REKAPLLHRYGGSPIGNSRVKLTNAADCVKRGLVKEGDNPLRVAAERLASDGITILHTIG
HHHCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEC
GDDTNTTAADLAAYLAANGYNLTVVGLPKTVDNDVVPIRQSLGAWTAAEVGAHFFDNVGN
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCC
EQTAAPRTLVIHEVMGRHCGWLTAATARAYLQRTSRNQYVDGLMMDAHLKSIDAVYLPEM
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCHH
AFDLDAEAARLKDSMDRNGHATVFVSEGACLDAIVAEREAAGETVKRDAFGHVKIDTINV
HHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHCCCCEEEEEEEEC
GAWFQKQFASLLNAERSLVQKSGYFARSAPANSDDLRLIQSMVDLAVESALNKVSGVTGH
CHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DEAQNGKLRTIEFPRIKGGKAFDLSTAWFAEVMDNIGQKYKEA
CCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653240; 1317210 [H]