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The map label for this gene is lipB [H]

Identifier: 209549465

GI number: 209549465

Start: 1917253

End: 1917966

Strand: Reverse

Name: lipB [H]

Synonym: Rleg2_1871

Alternate gene names: 209549465

Gene position: 1917966-1917253 (Counterclockwise)

Preceding gene: 209549473

Following gene: 209549464

Centisome position: 42.27

GC content: 64.43

Gene sequence:

>714_bases
ATGCTTCGCACCGATCTCGAATTCTCCATGCTCCCGAATCTCGGCAACCGCCCGGTGCGCTGGCGCATCGCCGATGGCCT
CGTTGCCTATGAGGAGGCGGTGGAGACGATGGAGCGCGAGGTGGCGGTGATATCAGAGGGCGGCGACGAACTCGTCTGGC
TCGTCGAGCATCCGCCGCTCTATACCGCGGGCACCAGCGCCAATGCCGAGGATCTCGTCCAGCCGGACCGGTTTCCGGTC
TTTGCGACGGGGCGCGGCGGTGAATATACCTATCACGGCCCCGGCCAGCGGGTCGCCTATGTGATGCTCGACCTGAAGCG
CAGGCGCCAGGACGTGCGCGCCTTCGTCGCCGCTCTGGAGGACGTCGTCATCCGCACGCTCGATATGATGAATGTGCGCG
GCGAACGGCGTGAAGATCGCGTCGGCGTCTGGGTGCGGCGGCCGGAAAAGCCGTTGCTTGCCGATGGAACGATGGCCGAG
GACAAGATCGCCGCCCTCGGGATCAGGCTGCGGAAATGGGTGACCTTCCACGGACTGTCGCTCAATGTCGATCCCGATCT
CGATCATTTCGGCGGTATCGTGCCCTGCGGCATCTCGGCCTATGGCGTGACCAGCCTGGTCGATCTTGGCTTGCCGGTGA
TGATGGCCGATGTCGATATGCGGCTGCGCACCGCCTTCGAGGCGGTTTTCGGCGAAACGACGGGCGAAAGCTGA

Upstream 100 bases:

>100_bases
AACCGGTCACAGCATTTGAATTGCCCCCGAAACGGGTCCATATAGGTCTCCTGCCGCTCCTTTTTTTCAGACATTTCGGA
GAGAGCGGCAAGGAATAGCC

Downstream 100 bases:

>100_bases
CGGCGATCGGACCTCAAGCTGCGGCACTCTGCCGTCAGGATCGGCAAAGCAAAAATCGCTGCTCCTTCAGATGTGAGCGA
AGGCCTTAACCTATAATTAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLRTDLEFSMLPNLGNRPVRWRIADGLVAYEEAVETMEREVAVISEGGDELVWLVEHPPLYTAGTSANAEDLVQPDRFPV
FATGRGGEYTYHGPGQRVAYVMLDLKRRRQDVRAFVAALEDVVIRTLDMMNVRGERREDRVGVWVRRPEKPLLADGTMAE
DKIAALGIRLRKWVTFHGLSLNVDPDLDHFGGIVPCGISAYGVTSLVDLGLPVMMADVDMRLRTAFEAVFGETTGES

Sequences:

>Translated_237_residues
MLRTDLEFSMLPNLGNRPVRWRIADGLVAYEEAVETMEREVAVISEGGDELVWLVEHPPLYTAGTSANAEDLVQPDRFPV
FATGRGGEYTYHGPGQRVAYVMLDLKRRRQDVRAFVAALEDVVIRTLDMMNVRGERREDRVGVWVRRPEKPLLADGTMAE
DKIAALGIRLRKWVTFHGLSLNVDPDLDHFGGIVPCGISAYGVTSLVDLGLPVMMADVDMRLRTAFEAVFGETTGES
>Mature_237_residues
MLRTDLEFSMLPNLGNRPVRWRIADGLVAYEEAVETMEREVAVISEGGDELVWLVEHPPLYTAGTSANAEDLVQPDRFPV
FATGRGGEYTYHGPGQRVAYVMLDLKRRRQDVRAFVAALEDVVIRTLDMMNVRGERREDRVGVWVRRPEKPLLADGTMAE
DKIAALGIRLRKWVTFHGLSLNVDPDLDHFGGIVPCGISAYGVTSLVDLGLPVMMADVDMRLRTAFEAVFGETTGES

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=157, Percent_Identity=35.6687898089172, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87081767, Length=174, Percent_Identity=40.2298850574713, Blast_Score=134, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323268, Length=232, Percent_Identity=25, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355483, Length=185, Percent_Identity=36.2162162162162, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26419; Mature: 26419

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTDLEFSMLPNLGNRPVRWRIADGLVAYEEAVETMEREVAVISEGGDELVWLVEHPPL
CCCCCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCE
YTAGTSANAEDLVQPDRFPVFATGRGGEYTYHGPGQRVAYVMLDLKRRRQDVRAFVAALE
EECCCCCCHHHHCCCCCCEEEEECCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DVVIRTLDMMNVRGERREDRVGVWVRRPEKPLLADGTMAEDKIAALGIRLRKWVTFHGLS
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHEEEEECEE
LNVDPDLDHFGGIVPCGISAYGVTSLVDLGLPVMMADVDMRLRTAFEAVFGETTGES
EECCCCHHHHCCEECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRTDLEFSMLPNLGNRPVRWRIADGLVAYEEAVETMEREVAVISEGGDELVWLVEHPPL
CCCCCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCE
YTAGTSANAEDLVQPDRFPVFATGRGGEYTYHGPGQRVAYVMLDLKRRRQDVRAFVAALE
EECCCCCCHHHHCCCCCCEEEEECCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
DVVIRTLDMMNVRGERREDRVGVWVRRPEKPLLADGTMAEDKIAALGIRLRKWVTFHGLS
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHEEEEECEE
LNVDPDLDHFGGIVPCGISAYGVTSLVDLGLPVMMADVDMRLRTAFEAVFGETTGES
EECCCCHHHHCCEECCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA