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The map label for this gene is yafC [H]

Identifier: 209549387

GI number: 209549387

Start: 1842711

End: 1843625

Strand: Reverse

Name: yafC [H]

Synonym: Rleg2_1793

Alternate gene names: 209549387

Gene position: 1843625-1842711 (Counterclockwise)

Preceding gene: 209549388

Following gene: 209549385

Centisome position: 40.63

GC content: 61.42

Gene sequence:

>915_bases
ATGGATCGTCTCGAAGCGATGCGCATATTGCTGGCCGTTGTCGACGCCGGAAGCATCTCGGCCGGCAGCAGGCGGCTGAA
CGCGCCCTTGCCCAGCGTCAGCCGCAAGGTGGCCGAGCTTGAACGCCATCTCGGCGCAAACCTCGTCGTCCGCACCAGTC
GCAATCTTCAGCTCACCGATGCCGGCCGCGACTATGTCGACGCCGCCCGAAAGATCATAGCGGATATCGATGAGGCGGAG
CGAAGGGCGTCGGGCGAATACCAGACGCCCAGAGGCGCGCTCACGATCACGATGCCGATCGAATATGGGAGCCGGTATGT
GCTGCCCGTTGCGCTCAGCTTCATGGACAAATACCCGGAGGTGTCGCTGAAGCTTCTGTCGCTCGACCGTACCGTCGATC
TTGTGTCCGAGCAGGTGGATATCGCCATCCGTCTCGGCGACCTCGCCGACAGTTCCCTCCATGCCGTCAAAACCGGCGAA
TTCCGCCTGCTGACCTGCGCGAGCCCTGCCTATCTCGCGCGGCATGGCGAACCTCAGCGCCCAGGTGATCTCATCGATCG
CGACGGGATCTTCTTCAACAAGCCGGCATTCTTCTGGACATTCGACATCGAGGGAAAGCCGATGGAGGCGATGCCCCGCA
ACCGGCTGGAAGTGAATACCGCGGCGAACTGCGTCGCAGCCGCCGTCGATGGAGCAGGGATTGCGCGGCTCTTCGACTAC
CAGGTTCCAGACCAGCTGGCGTCCGGCGCGCTGGTGCCTATCCTGCGGGATTTCGATGGACCACCACAGCCCATTCATAT
CGTTTACGCGCGCCAGGGGATTCTGGCGCTGAAAGTCCGCGCTTTTGTCGACTGGGCTCTGCCCCGGTTGCGCGCGCCAT
CTAAAATCTTTGATAATCAAATCTTATCCGAATGA

Upstream 100 bases:

>100_bases
CATACGCAGGCTGCTGGATTAGGATTGCCTGCGGTTCAGGGCAACGTCATAGTCTGCTGGCCGATGATGAGCAGAAGCAA
TATCTTACGGGCAGTGGCCA

Downstream 100 bases:

>100_bases
TGCACGAATTTCTTCAACCGTCTGTTTCGCGTTTTGAAGATGAGGAATGTGGCCGATGCCCGGCAGGATATCGATTTTCA
CACCGATCCTGGCGGCGAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDRLEAMRILLAVVDAGSISAGSRRLNAPLPSVSRKVAELERHLGANLVVRTSRNLQLTDAGRDYVDAARKIIADIDEAE
RRASGEYQTPRGALTITMPIEYGSRYVLPVALSFMDKYPEVSLKLLSLDRTVDLVSEQVDIAIRLGDLADSSLHAVKTGE
FRLLTCASPAYLARHGEPQRPGDLIDRDGIFFNKPAFFWTFDIEGKPMEAMPRNRLEVNTAANCVAAAVDGAGIARLFDY
QVPDQLASGALVPILRDFDGPPQPIHIVYARQGILALKVRAFVDWALPRLRAPSKIFDNQILSE

Sequences:

>Translated_304_residues
MDRLEAMRILLAVVDAGSISAGSRRLNAPLPSVSRKVAELERHLGANLVVRTSRNLQLTDAGRDYVDAARKIIADIDEAE
RRASGEYQTPRGALTITMPIEYGSRYVLPVALSFMDKYPEVSLKLLSLDRTVDLVSEQVDIAIRLGDLADSSLHAVKTGE
FRLLTCASPAYLARHGEPQRPGDLIDRDGIFFNKPAFFWTFDIEGKPMEAMPRNRLEVNTAANCVAAAVDGAGIARLFDY
QVPDQLASGALVPILRDFDGPPQPIHIVYARQGILALKVRAFVDWALPRLRAPSKIFDNQILSE
>Mature_304_residues
MDRLEAMRILLAVVDAGSISAGSRRLNAPLPSVSRKVAELERHLGANLVVRTSRNLQLTDAGRDYVDAARKIIADIDEAE
RRASGEYQTPRGALTITMPIEYGSRYVLPVALSFMDKYPEVSLKLLSLDRTVDLVSEQVDIAIRLGDLADSSLHAVKTGE
FRLLTCASPAYLARHGEPQRPGDLIDRDGIFFNKPAFFWTFDIEGKPMEAMPRNRLEVNTAANCVAAAVDGAGIARLFDY
QVPDQLASGALVPILRDFDGPPQPIHIVYARQGILALKVRAFVDWALPRLRAPSKIFDNQILSE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=32.9824561403509, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789639, Length=286, Percent_Identity=28.3216783216783, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=28.9655172413793, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=26.6666666666667, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081978, Length=284, Percent_Identity=29.2253521126761, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=24.6666666666667, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787589, Length=266, Percent_Identity=27.4436090225564, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=23.7623762376238, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=26, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787601, Length=163, Percent_Identity=28.2208588957055, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33521; Mature: 33521

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLEAMRILLAVVDAGSISAGSRRLNAPLPSVSRKVAELERHLGANLVVRTSRNLQLTD
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEC
AGRDYVDAARKIIADIDEAERRASGEYQTPRGALTITMPIEYGSRYVLPVALSFMDKYPE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCC
VSLKLLSLDRTVDLVSEQVDIAIRLGDLADSSLHAVKTGEFRLLTCASPAYLARHGEPQR
CEEEEEEHHHHHHHHHHCEEEEEEECCCCCCCEEEEECCCEEEEEECCCHHHHHCCCCCC
PGDLIDRDGIFFNKPAFFWTFDIEGKPMEAMPRNRLEVNTAANCVAAAVDGAGIARLFDY
CCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHCC
QVPDQLASGALVPILRDFDGPPQPIHIVYARQGILALKVRAFVDWALPRLRAPSKIFDNQ
CCCHHHHCCCEEHHHHCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHHHH
ILSE
HCCC
>Mature Secondary Structure
MDRLEAMRILLAVVDAGSISAGSRRLNAPLPSVSRKVAELERHLGANLVVRTSRNLQLTD
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEC
AGRDYVDAARKIIADIDEAERRASGEYQTPRGALTITMPIEYGSRYVLPVALSFMDKYPE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCC
VSLKLLSLDRTVDLVSEQVDIAIRLGDLADSSLHAVKTGEFRLLTCASPAYLARHGEPQR
CEEEEEEHHHHHHHHHHCEEEEEEECCCCCCCEEEEECCCEEEEEECCCHHHHHCCCCCC
PGDLIDRDGIFFNKPAFFWTFDIEGKPMEAMPRNRLEVNTAANCVAAAVDGAGIARLFDY
CCCCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHCC
QVPDQLASGALVPILRDFDGPPQPIHIVYARQGILALKVRAFVDWALPRLRAPSKIFDNQ
CCCHHHHCCCEEHHHHCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHHHH
ILSE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]