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The map label for this gene is potD [C]

Identifier: 209549357

GI number: 209549357

Start: 1802704

End: 1803729

Strand: Reverse

Name: potD [C]

Synonym: Rleg2_1761

Alternate gene names: 209549357

Gene position: 1803729-1802704 (Counterclockwise)

Preceding gene: 209549358

Following gene: 209549356

Centisome position: 39.75

GC content: 60.23

Gene sequence:

>1026_bases
ATGCGACTGACAGTTCTTTCCACGCTTCTCTTTGCCGGAACGGCGCTGGCTGCCGGTTCGGTCCAGGCCGCCGGCGAGCT
CAACCTCATCTGCTCGGCCGATGTCGTCATCTGCGAGCAGATGCAGGGCGACTTTGAAAAGGCTCACGACATCAAGGTGA
ATATGGTGCGCCTGTCATCGGGCGAGACCTATGCCAAGATACGCGCCGAGGCTCGCAACCCGAAGACCGACATCTGGTGG
GCCGGCACGGGGGATCCGCATGTCCAGGCAGCATCGGAAAACCTGACGCTGGAATACAAGTCGCCAATGCTCGACCAGTT
GCAGGATTGGGCCAAAAAGCAGGCGGAGAGCACCGGTTTCAAGACGGTCGGCGTCTACGCCGGTGCGCTCGGCTGGGGTT
ACAACACCGAGATCTTCAAGACCAAGGGTTACAAGGAACCCCGCTGCTGGGCCGATCTCCTGGCGCCGGAATTGAAGGGC
GAAATCCAGATCGCCAATCCGAACTCCTCGGGCACTGCCTATACGGCACTCGCTTCGCTGGTGCAGATCATGGGCGAAGA
CCAGGCCATCGACTATCTGAAAAAACTAAACGCCAACGTCTCGCAATACACCAAATCCGGATCGGCTCCTGTCAAGGCGG
CGGCGCGGGGCGAAACGGCCCTCGGCATCGTCTTCATGCATGACGCCGTCGCGCAGACCGCCGAAGGTTTTCCGGTCAAG
TCGGTCGCGCCATGCGAGGGCACCGGCTATGAAATCGGCTCCATGTCGATCATTAAAGGCGCCCGCAATCTCGACAATGC
CAAGATCTGGTACGACTGGGCGCTGCGACCCGAAGTGCAGTCGCGCATGAAGGATGCCAAGTCCTTCCAGCTGCCGTCGA
ACAAATCGGCGGAGGTGCCGAAGGAAGCGCCGAAGTTCGAGGACATCAAGCTGATCGACTACGACTTCAAGACCTATGGC
GATCCGGCAAAGCGCAAGGCCCTGCTCGAGCGCTGGGATCGGGAAGTCGGCGCCATCGCCAACTGA

Upstream 100 bases:

>100_bases
ATCCGACCCCTGACAACCCGGTGCGATCGACGCGCCGCGACGGGCAGCGCTTGTGTCGCTGACCCACCCCCTCTCAGACG
CAAGCAACAGGAGGATACCC

Downstream 100 bases:

>100_bases
AGATCGATGAGTGGCACGGCATCGCGGACGGTTCCGGGGTGCCGATCCATTCTCCCCTTTCATCAACGTGAAATAAGCGA
GGTATGCCATGAGACGTGGC

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MRLTVLSTLLFAGTALAAGSVQAAGELNLICSADVVICEQMQGDFEKAHDIKVNMVRLSSGETYAKIRAEARNPKTDIWW
AGTGDPHVQAASENLTLEYKSPMLDQLQDWAKKQAESTGFKTVGVYAGALGWGYNTEIFKTKGYKEPRCWADLLAPELKG
EIQIANPNSSGTAYTALASLVQIMGEDQAIDYLKKLNANVSQYTKSGSAPVKAAARGETALGIVFMHDAVAQTAEGFPVK
SVAPCEGTGYEIGSMSIIKGARNLDNAKIWYDWALRPEVQSRMKDAKSFQLPSNKSAEVPKEAPKFEDIKLIDYDFKTYG
DPAKRKALLERWDREVGAIAN

Sequences:

>Translated_341_residues
MRLTVLSTLLFAGTALAAGSVQAAGELNLICSADVVICEQMQGDFEKAHDIKVNMVRLSSGETYAKIRAEARNPKTDIWW
AGTGDPHVQAASENLTLEYKSPMLDQLQDWAKKQAESTGFKTVGVYAGALGWGYNTEIFKTKGYKEPRCWADLLAPELKG
EIQIANPNSSGTAYTALASLVQIMGEDQAIDYLKKLNANVSQYTKSGSAPVKAAARGETALGIVFMHDAVAQTAEGFPVK
SVAPCEGTGYEIGSMSIIKGARNLDNAKIWYDWALRPEVQSRMKDAKSFQLPSNKSAEVPKEAPKFEDIKLIDYDFKTYG
DPAKRKALLERWDREVGAIAN
>Mature_341_residues
MRLTVLSTLLFAGTALAAGSVQAAGELNLICSADVVICEQMQGDFEKAHDIKVNMVRLSSGETYAKIRAEARNPKTDIWW
AGTGDPHVQAASENLTLEYKSPMLDQLQDWAKKQAESTGFKTVGVYAGALGWGYNTEIFKTKGYKEPRCWADLLAPELKG
EIQIANPNSSGTAYTALASLVQIMGEDQAIDYLKKLNANVSQYTKSGSAPVKAAARGETALGIVFMHDAVAQTAEGFPVK
SVAPCEGTGYEIGSMSIIKGARNLDNAKIWYDWALRPEVQSRMKDAKSFQLPSNKSAEVPKEAPKFEDIKLIDYDFKTYG
DPAKRKALLERWDREVGAIAN

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37227; Mature: 37227

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTVLSTLLFAGTALAAGSVQAAGELNLICSADVVICEQMQGDFEKAHDIKVNMVRLSS
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHCCEEEEEEEECC
GETYAKIRAEARNPKTDIWWAGTGDPHVQAASENLTLEYKSPMLDQLQDWAKKQAESTGF
CCEEEEEEECCCCCCCCEEECCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KTVGVYAGALGWGYNTEIFKTKGYKEPRCWADLLAPELKGEIQIANPNSSGTAYTALASL
EEEHEEEHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
VQIMGEDQAIDYLKKLNANVSQYTKSGSAPVKAAARGETALGIVFMHDAVAQTAEGFPVK
HHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCEEEEEEEHHHHHHHCCCCCCC
SVAPCEGTGYEIGSMSIIKGARNLDNAKIWYDWALRPEVQSRMKDAKSFQLPSNKSAEVP
CCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC
KEAPKFEDIKLIDYDFKTYGDPAKRKALLERWDREVGAIAN
CCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLTVLSTLLFAGTALAAGSVQAAGELNLICSADVVICEQMQGDFEKAHDIKVNMVRLSS
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHCCEEEEEEEECC
GETYAKIRAEARNPKTDIWWAGTGDPHVQAASENLTLEYKSPMLDQLQDWAKKQAESTGF
CCEEEEEEECCCCCCCCEEECCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KTVGVYAGALGWGYNTEIFKTKGYKEPRCWADLLAPELKGEIQIANPNSSGTAYTALASL
EEEHEEEHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
VQIMGEDQAIDYLKKLNANVSQYTKSGSAPVKAAARGETALGIVFMHDAVAQTAEGFPVK
HHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCCEEEEEEEHHHHHHHCCCCCCC
SVAPCEGTGYEIGSMSIIKGARNLDNAKIWYDWALRPEVQSRMKDAKSFQLPSNKSAEVP
CCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC
KEAPKFEDIKLIDYDFKTYGDPAKRKALLERWDREVGAIAN
CCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]