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The map label for this gene is ybhO

Identifier: 209400411

GI number: 209400411

Start: 948230

End: 949471

Strand: Reverse

Name: ybhO

Synonym: ECH74115_0937

Alternate gene names: 209400411

Gene position: 949471-948230 (Counterclockwise)

Preceding gene: 209396778

Following gene: 209400944

Centisome position: 17.04

GC content: 54.43

Gene sequence:

>1242_bases
ATGAAATGTAGCTGGCGCGAAGGCAATAAGATCCAGTTGCTGGAAAACGGCGAGCAATATTATCCCGCGGTGTTTAAGGC
GATTGGCGAGGCACAAGAACGCATCATTCTTGAAACGTTTATCTGGTTTGAGGATGACGTCGGCAAACAACTGCATGCGG
CACTACTGGCAGCAGCGCAACGCGGGGTTAAAGCGGAAGTCTTGCTGGATGGCTACGGTTCGCCGGATCTCAGCGATGAG
TTTGTCAATGAACTGACGGCAGCTGGCGTAGTGTTCCGCTACTACGATCCCCGCCCTCGCCTTTTTGGTATGCGCACCAA
TGTGTTTCGCCGGATGCATCGCAAAATTGTGGTAATCGACGCGCGTATCGCCTTTATTGGCGGGCTGAATTACTCCGCCG
AGCATATGTCCAGCTACGGTCCAGAGGCTAAACAGGATTACGCGGTACGCCTTGAAGGGCCGATTGTCGAAGATATTCTC
CAGTTTGAGCTGGAAAACCTGCCTGGACAGAGCGCGGCACGACGCTGGTGGCGACGTCATCACAAAGCGGAAGAGAACCG
CCAGCCGGGAGAAGCGCAGGTATTGCTGGTCTGGCGCGATAACGAAGAACATCGCGATGATATTGAACGTCACTATCTGA
AAATGCTCACTCAGGCGCGGCGAGAAGTCATTATCGCCAACGCCTACTTCTTCCCCGGCTATCGATTTTTACACGCCTTG
CGTAAAGCGGCACGGCGCGGGGTGCGGATCAAACTGATCATTCAGGGCGAACCGGATATGCCGATTGTCAGAGTCGGTGC
GCGCTTGCTGTATAACTATCTGGTTAAAGGCGGCGTTCAGGTGTTTGAGTACCGCCGCCGCCCGCTCCACGGCAAAGTGG
CATTGATGGACGATCACTGGGCGACAGTAGGGTCCAGTAATCTCGATCCGCTCAGTTTGTCACTGAATCTCGAAGCAAAT
GTCATCATCCACGATCGTCATTTTAACCAGACGCTGCGCGATAATCTGAACGGCATTATTGCCGCAGATTGTCAGCAGGT
CGATGAGACCATGCTGCCGAAACGCACCTGGTGGAACCTAACCAAAAGCGTGCTGGCGTTCCACTTTTTACGCCACTTCC
CGGCGCTGGTTGGCTGGCTTCCGGCACACACGCCACGTCTGGCGCAGGTTGATCCTCCCGCACAACCGACAATGGAAACG
CAGGATCGGGTAGAAACTGAAAACACGGGGGTAAAACCCTGA

Upstream 100 bases:

>100_bases
TCTACGTCAAAAATACCAGCGCCAGCGCGCCAACCGCGTTGCCGCTGCGGACATGGCGACACCTTTCTGATCATGCCCCT
TTAAGTGCGGAGATTCATTT

Downstream 100 bases:

>100_bases
TGAGTAAATCACACCCGCGCTGGCGCTTAGCAAAGAAGATCCTCACCTGGCTGTTTTTTATCGCGGTGATTGTGTTGCTG
GTGGTCTACGCCAAAAAAGT

Product: cardiolipin synthase 2

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDE
FVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHAL
RKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEAN
VIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMET
QDRVETENTGVKP

Sequences:

>Translated_413_residues
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDE
FVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHAL
RKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEAN
VIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMET
QDRVETENTGVKP
>Mature_413_residues
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDE
FVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHAL
RKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEAN
VIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMET
QDRVETENTGVKP

Specific function: Catalyzes, in vitro, the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787007, Length=413, Percent_Identity=100, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1787502, Length=400, Percent_Identity=25.25, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87081826, Length=399, Percent_Identity=23.0576441102757, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBHO_ECO57 (P0AA85)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90737
- PIR:   D85587
- RefSeq:   NP_286552.1
- RefSeq:   NP_308894.1
- ProteinModelPortal:   P0AA85
- EnsemblBacteria:   EBESCT00000027920
- EnsemblBacteria:   EBESCT00000060244
- GeneID:   917609
- GeneID:   958012
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1008
- KEGG:   ecs:ECs0867
- GeneTree:   EBGT00050000009472
- HOGENOM:   HBG694312
- OMA:   EYCQRPL
- ProtClustDB:   PRK11263
- BioCyc:   ECOL83334:ECS0867-MONOMER
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 47634; Mature: 47634

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 113-113 ACT_SITE 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQ
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVID
CCCEEEEEEECCCCCCCCHHHHHHHHHHCEEEEEECCCCCEECHHHHHHHHHCCEEEEEE
ARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDILQFELENLPGQSAARRWWRRH
EEEEEEECCCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHH
HKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHAL
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHH
RKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHW
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEECCCC
ATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNL
CCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHH
TKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMETQDRVETENTGVKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQ
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVID
CCCEEEEEEECCCCCCCCHHHHHHHHHHCEEEEEECCCCCEECHHHHHHHHHCCEEEEEE
ARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDILQFELENLPGQSAARRWWRRH
EEEEEEECCCCCHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHH
HKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHAL
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHH
RKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHW
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEECCCC
ATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNL
CCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHH
TKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMETQDRVETENTGVKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796