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The map label for this gene is ycdT [H]

Identifier: 209400191

GI number: 209400191

Start: 1278033

End: 1279463

Strand: Direct

Name: ycdT [H]

Synonym: ECH74115_1267

Alternate gene names: 209400191

Gene position: 1278033-1279463 (Clockwise)

Preceding gene: 209400137

Following gene: 209399126

Centisome position: 22.94

GC content: 38.23

Gene sequence:

>1431_bases
TTGACAACAGTACAGTTCTTATATCTATTAATAATAGAAAGGGATCTACAACCTACAGATTGGTGTAGCTTTATGGAAAA
AGACTATTTGAGAATTAGTAGTACTGTATTAGTGAGCTTATTGTTTGGGCTTGCTTTGGTTTTGGTGAATAGTTGGTTTA
ATCAGCCAGGCGTTGAGGAAGTTGTTCCACGATCAACCTATCTGATGGTAATGATTGCTTTATTTTTTATCGATACTGTT
GCATTTATTTTTATGCAGTTGTATTTCATTTATGACCGTAGGCAATTTTCAAACTGTATACTTAGTTTGGCTTTTCTGAG
TTGTTTGATTTACTTTGTTAAAACCGTCATTATCATTCAGCAAATTATTGAGGGGCGTTTGACAAGCAGTGTTGTCCAAA
ATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGT
GAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGGCGAT
AGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGG
TTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTA
CGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATTTATGAGTAG
GTATAGCGTGTCCACTTGGTATATTAGTCGCACTATTGAAGTAGTCAGTAAGTTAACCGTTATGGTAATATTTATGTGCC
ATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTT
TTTAATGAACTGACAGTTCAATCAGCATCAGCCAAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAA
AAAAGTCAACGACACCTGGGGGCATCCGGTTGGCGATCAGGTGATAAAAACAGTGGTGAATATCATTGGGAAAAGCATAC
GACCAGATGACCTTTTAGCGCGCGTCGGCGGCGAAGAGTTTGGCGTCTTGCTGACGGACATCGATACTGAACGTGCGAAA
GCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGAC
GATTAGTATTGGCGCTGTTGTGACTCAGGGAAATGCGTTAAACCCGAATGAGATCTATCGACTGGCTGATAACGCACTAT
ACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTGAGTCACCATAA

Upstream 100 bases:

>100_bases
CAAGCCTGGCTTGTTGCAAACAATGTATAAAGCACTTAGGCAATAATAATTACATTCAGCAACTATCATCATCGGTATTG
TTTGTGGGCGGAATTTCATA

Downstream 100 bases:

>100_bases
AGCGGCATTTTAGCGGGTGATTTTGGTAAGCGCTAAATAGCTATTCGCAGACATGCGTAACAATTTTCAGAAATATCATA
GTACTATATGATCTTAAATG

Product: diguanylate cyclase (GGDEF) domain protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTV
AFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
ENTKQRNLFSKKMTLCISLFFVFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYF
FNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAK
ALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP

Sequences:

>Translated_476_residues
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTV
AFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
ENTKQRNLFSKKMTLCISLFFVFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYF
FNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAK
ALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP
>Mature_475_residues
TTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVA
FIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSE
NTKQRNLFSKKMTLCISLFFVFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLR
YDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFF
NELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKA
LAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=452, Percent_Identity=98.6725663716814, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1788085, Length=449, Percent_Identity=37.8619153674833, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=35.7142857142857, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=34.7058823529412, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=34.3373493975904, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=217, Percent_Identity=31.7972350230415, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=32.183908045977, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=31.0975609756098, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=28.8235294117647, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=245, Percent_Identity=28.1632653061224, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 54558; Mature: 54426

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGL
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
RYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQ
HHHHHHHCCHHHHHHCCCCEEHHHEEECCCCCCCCEEEEEEEHHHHHHCCCCCCCCCHHH
VIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEY
HHHHHHHHHCCCCCHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
AIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP
CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEE
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH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CCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]