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The map label for this gene is rtn [H]

Identifier: 209399126

GI number: 209399126

Start: 1279657

End: 1281171

Strand: Direct

Name: rtn [H]

Synonym: ECH74115_1268

Alternate gene names: 209399126

Gene position: 1279657-1281171 (Clockwise)

Preceding gene: 209400191

Following gene: 209396374

Centisome position: 22.97

GC content: 36.37

Gene sequence:

>1515_bases
ATGGTATTCAACAAAAAAATGTTTGTACTCATTATAATACCAGGCATTCTGGGGGTGTTACTGTCGTTTGCTATGTCAGT
ATTTCAAATGAATCGAGATACGACAATTACCGCCGGTATTCTGTTAAAACAGCTTGATAATGTTACGCAAATTGTCAAAC
ATACGACAAAGCTTACTTCTATTCTGGTGATGAAACCCTGCAAAGATATCCTGGAACAATTGATTGCTAACGGAGCATTG
ACCCCCTATGTTCGAACAACGGGTCTTATAGAAAATAATTTCCAAATATGTTCTTCAGTTAGCGGTTTCAAAAAAATGAA
TGTTAACGATGTTTACGGTACGAGCTTTCACAATAAAAATAAAGAATCACGCATAGTATCAATTAGCGGTACAAGTTTTG
TACCAGGTAAAACAGCCATAGTGTTCCTTATGCCAATAGGGAATGATATGACAGCTTTTAGTATTGTAGAGTCACGATAT
ATCTATGATCTGATGGATGTTCTTGATGATGAGAATGATGATTCATTTTCTCTTCGCTTTACTGAAGGGCCTGCAATTAT
TAGTGGTGTAAATAATAATGACAGGTTATATATGTTAAAAAAAGATTTTAATTCTGCGATCAGTCAGGCTAGTCTTACAG
TTACTACGCCGATGATTTCATTATATCCCTATGTCATTAGTAATGTATTTTACATTCTTCCATTATCTATTCTTCTTTCT
TTTATCCTGTACTTCTTATGGCAGCGCTGGATATCACGGAAAATGTCCCTGGCAGAAGAAATTAAGAAAGGAATGTCATC
CGGAGAATTTTCTGTGCATTATCAACCAGTATGTGACACGACAACCAAAGCGTGTCTGGGGGTTGAGGCGTTAATGCGTT
GGCAACGCGAAGATGGTAAAAATATATCTCCGGTAGTTTTTATCCGCGCTGCAGAAGAGGAAAATTTAATTATACCGTTA
ACTAAGCATCTCTTTGAACTGATTATTCAGGATGTACAAAGCTGGAAAGTGAAAAAGCCGTTTCACCTTGGGATAAATAT
TGCCGCTGAACATCTTGCACATCCTGATTTTGTTGCTGATGTTTTACATATAAAAAATGCAATATCTGATAAATTTAACA
TTGTCCTTGAGATTACCGAACGCAATCTGGTTGAAGATACTGATCATGCATTACAGAAAATAAATGAATTAAGGAGCCAT
GGCTGTGAATTTGCAGTTGATGATTTTGGCACGGGATATTGTTCCCTTGGCCTTCTGCAAAAGTTGTCTGTTGATTATCT
TAAAATAGATAAAAGCTTTATAGACACGCTCACTACAGCGGAAGACGAAACGCCTGTTTTAGATATTATTATTAAGCTGA
GTAATCGACTCAATTTGATAACGATTGCAGAAGGTGTCAGTACCTCTCACCAGGCGGAATATCTTATTCATAATAACGTG
ACATTAGTTCAGGGATACTTATATGCAAAACCAATGAAAGCGACAGAGTTTTATCAATGGTATGTAAACAGATAA

Upstream 100 bases:

>100_bases
TTAAATGCTTATCATTGATCAGTCTGCTATCACCCGCTTTTACTGGCAATTGTACTATAATTTCTTAGGTTGCATCACCC
TGATGCGTGGGGAAACATGT

Downstream 100 bases:

>100_bases
ATAATTAACCAATATTGGAATGGTTTTTTATTGCCTTAAAATATAAACAAAGCCATCAGAAGGTTATTAACTGGTGACAA
ACGCCAGGAGCAACAATGCT

Product: cyclic diguanylate phosphodiesterase (EAL) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MVFNKKMFVLIIIPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDILEQLIANGAL
TPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSIVESRY
IYDLMDVLDDENDDSFSLRFTEGPAIISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLS
FILYFLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNISPVVFIRAAEEENLIIPL
TKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVADVLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSH
GCEFAVDDFGTGYCSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNV
TLVQGYLYAKPMKATEFYQWYVNR

Sequences:

>Translated_504_residues
MVFNKKMFVLIIIPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDILEQLIANGAL
TPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSIVESRY
IYDLMDVLDDENDDSFSLRFTEGPAIISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLS
FILYFLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNISPVVFIRAAEEENLIIPL
TKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVADVLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSH
GCEFAVDDFGTGYCSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNV
TLVQGYLYAKPMKATEFYQWYVNR
>Mature_504_residues
MVFNKKMFVLIIIPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDILEQLIANGAL
TPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSIVESRY
IYDLMDVLDDENDDSFSLRFTEGPAIISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLS
FILYFLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNISPVVFIRAAEEENLIIPL
TKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVADVLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSH
GCEFAVDDFGTGYCSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNV
TLVQGYLYAKPMKATEFYQWYVNR

Specific function: Involved in resistance to phages N4 and lambda [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788502, Length=511, Percent_Identity=31.5068493150685, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87081845, Length=279, Percent_Identity=35.84229390681, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081980, Length=247, Percent_Identity=38.8663967611336, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI87081743, Length=277, Percent_Identity=34.6570397111913, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790496, Length=293, Percent_Identity=33.1058020477816, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786507, Length=247, Percent_Identity=35.6275303643725, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081921, Length=256, Percent_Identity=30.859375, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI226510982, Length=269, Percent_Identity=30.8550185873606, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787055, Length=248, Percent_Identity=33.0645161290323, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788849, Length=246, Percent_Identity=26.4227642276423, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082096, Length=243, Percent_Identity=24.2798353909465, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787410, Length=118, Percent_Identity=28.8135593220339, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 56948; Mature: 56948

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFNKKMFVLIIIPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTS
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILVMKPCKDILEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKN
HHHHHHHHHHHHHHHHCCCCCCCHHCCCEECCCHHHHHCCCCHHCCCCCCCCCCCCCCCC
KESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRF
CCCEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TEGPAIISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLS
ECCCEEEECCCCCCEEEEEHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FILYFLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
NISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
CCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHH
VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGLLQ
HHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
KLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNV
HHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCE
TLVQGYLYAKPMKATEFYQWYVNR
EEEEEEHEECCCCHHHHHHHEECC
>Mature Secondary Structure
MVFNKKMFVLIIIPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTS
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILVMKPCKDILEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKN
HHHHHHHHHHHHHHHHCCCCCCCHHCCCEECCCHHHHHCCCCHHCCCCCCCCCCCCCCCC
KESRIVSISGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRF
CCCEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TEGPAIISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLS
ECCCEEEECCCCCCEEEEEHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FILYFLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
NISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
CCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHH
VLHIKNAISDKFNIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGLLQ
HHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHH
KLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNV
HHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCE
TLVQGYLYAKPMKATEFYQWYVNR
EEEEEEHEECCCCHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 9079933 [H]