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The map label for this gene is hycI

Identifier: 209400052

GI number: 209400052

Start: 3670702

End: 3671172

Strand: Reverse

Name: hycI

Synonym: ECH74115_3968

Alternate gene names: 209400052

Gene position: 3671172-3670702 (Counterclockwise)

Preceding gene: 209399984

Following gene: 209396524

Centisome position: 65.89

GC content: 54.35

Gene sequence:

>471_bases
GTGACTGACGTTTTACTCTGTGTTGGCAATAGCATGATGGGTGATGATGGCGCAGGTCCGCTGCTGGCGGAAAAGTGCGC
CGCCGCGCCGAAAGGTAACTGGGTGGTGATTGACGGCGGTAGCGCACCGGAAAACGACATTGTCGCTATCCGCGAACTGC
GCCCGACACGGCTGCTGATTGTCGACGCCACGGATATGGGGCTAAACCCCGGCGAAATCCGCATCATCGACCCGGATGAT
ATCGCCGAGATGTTTATGATGACTACCCATAACATGCCGTTGAACTACCTTATCGATCAGTTGAAAGAAGATATTGGCGA
AGTGATTTTCCTCGGCATCCAGCCGGATATCGTCGGCTTTTACTACCCGATGACCCAGCCGATTAAAGATGCGGTAGAAA
CCGTTTATCAACGACTGGAAGGCTGGGAAGGGAACGGCGGCTTCGCGCAGTTAGCGGTGGAAGAAGAGTAG

Upstream 100 bases:

>100_bases
GCGCAAACTTCGCAGCAGCAGGAATGGAGTACAATGCTGTTAAGTATGCTGCATGATATTCATCAGGAAAACGCCATCTA
TTTGATGGTGAGGAGACTGC

Downstream 100 bases:

>100_bases
TTTTTCAATAAGTAATCAGGACAGGGATGTTCTTGATGAGGGAATCAGAACTGATAGCAAACCCCAGATTGCCCGATGCG
CAACGCTTATCGGGCCTTCC

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE

Sequences:

>Translated_156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE
>Mature_155_residues
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDI
AEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family

Homologues:

Organism=Escherichia coli, GI1789072, Length=156, Percent_Identity=100, Blast_Score=317, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCI_ECO57 (P0AEW0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85920
- PIR:   E91075
- RefSeq:   NP_289266.1
- RefSeq:   NP_311600.1
- ProteinModelPortal:   P0AEW0
- SMR:   P0AEW0
- MINT:   MINT-1244218
- MEROPS:   A31.003
- EnsemblBacteria:   EBESCT00000024097
- EnsemblBacteria:   EBESCT00000055296
- GeneID:   914705
- GeneID:   959495
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4025
- KEGG:   ecs:ECs3573
- GeneTree:   EBGT00050000009077
- HOGENOM:   HBG730254
- OMA:   MPLNYLV
- ProtClustDB:   PRK11544
- BioCyc:   ECOL83334:ECS3573-MONOMER
- InterPro:   IPR004420
- InterPro:   IPR000671
- Gene3D:   G3DSA:3.40.50.1450
- PRINTS:   PR00446
- TIGRFAMs:   TIGR00142
- TIGRFAMs:   TIGR00072

Pfam domain/function: PF01750 HycI

EC number: =3.4.23.51

Molecular weight: Translated: 17057; Mature: 16926

Theoretical pI: Translated: 3.79; Mature: 3.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEE
YYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEE
YYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE
ECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796