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The map label for this gene is ascG [H]

Identifier: 209396524

GI number: 209396524

Start: 3666418

End: 3667470

Strand: Reverse

Name: ascG [H]

Synonym: ECH74115_3965

Alternate gene names: 209396524

Gene position: 3667470-3666418 (Counterclockwise)

Preceding gene: 209400052

Following gene: 209397110

Centisome position: 65.82

GC content: 52.33

Gene sequence:

>1053_bases
GTGCAGTACACTGCGTTGATTGCGTGTAAAGGGATGAAAATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGT
TTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGGCTACGTCAGCCAGGAGACTAAAGATCGCGTGTTTCAGGCGG
TAGAAGAGAGCGGTTACCGTCCAAACTTGCTGGCGCGCAATCTGTCGGCGAAAAGTACCCAGACGCTGGGGCTGGTAGTG
ACTAACACGCTTTACCACGGCATTTATTTTAGTGAATTACTGTTTCATGCCGCGCGAATGGCGGAAGAGAAAGGGCGGCA
GTTGCTATTGGCAGATGGTAAACACAGCGCCGAAGAAGAGCGCCAGGCGATTCAGTATCTGCTGGATTTGCGCTGCGACG
CCATTATGATTTACCCGCGTTTTTTAAGTGTGGATGAGATCGATGACATCATTGACGCGCACAGTCAGCCGATAATGGTG
CTTAATCGCCGCCTGCGCAAAAATAGCAGCCATAGCGTCTGGTGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGA
GTTGATAAACGCCGGGCATCAGGAGATTGCTTTTCTTACCGGCTCGATGGATTCCCCCACCAGCGTTGAACGTCTTGCCG
GATATAAAGACGCGCTGGCGCAGCATGGTATTGCGCTCAATGAAAAACTTATCGCTAACGGTAAATGGACGCCCGCCAGC
GGGGCCGAAGGGGTAGAAACGTTGCTCGAACGTGGGGCTAAATTTAGCGCGTTAGTTGCCAGTAACGACGATATGGCGAT
AGGTGCGATGAAAGCGTTACACAAGCGCGGCGTAGCGGTGCCAGAGCAGGTGTCAGTTATCGGATTCGATGATATCGCAA
TTGCCCCCTATATCGTTCCGGCGCTCTCCAGCGTAAAAATTCCGGTGACTGAGATGATTCAGGAAATTATTGGACGGCTG
ATTTTTATGCTCGATGGCGGGGATTTCTCACCGCCGAAAACCTTCAGCGGAAAACTGATCCGCCGCGACTCACTCATTGC
TCTTTCGCGATAA

Upstream 100 bases:

>100_bases
AACCAAATAAGCAAGAGAGTTAATTGTTCATTTTTTATATTTGTGAAACCGGTCACCTGAATATTTTTCCCGCCGATTGC
GCAAGGACAATCGGTAAACG

Downstream 100 bases:

>100_bases
ACATTGTCATCACATGTCATCGTCACTAGTGTCGATGACATCTTTTTTCCTGCATTACAGAAATTAATTTCCTTAAATAA
CAGCAAATTAAAAACTGGCA

Product: transcriptional regulator AscG

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRDSLIALSR

Sequences:

>Translated_350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRDSLIALSR
>Mature_350_residues
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVV
TNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMV
LNRRLRKNSSHSVWCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPAS
GAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRL
IFMLDGGDFSPPKTFSGKLIRRDSLIALSR

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=98.5163204747775, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.3293413173653, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=33.6336336336336, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790369, Length=318, Percent_Identity=30.188679245283, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787948, Length=301, Percent_Identity=29.9003322259136, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=29.1021671826625, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=27.6872964169381, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=27.9761904761905, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787906, Length=284, Percent_Identity=30.2816901408451, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=25.0847457627119, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=24.6913580246914, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789456, Length=299, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=24.6710526315789, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38368; Mature: 38368

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYR
CCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEE
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
RQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQT
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCC
SFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPAS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
GAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVP
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIALSR
HHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEECCCEEEECC
>Mature Secondary Structure
MQYTALIACKGMKMMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYR
CCCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
PNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEE
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
RQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQT
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCC
SFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPAS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
GAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVP
CCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHH
ALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIALSR
HHCCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]