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The map label for this gene is malS [H]

Identifier: 209399658

GI number: 209399658

Start: 4586684

End: 4588714

Strand: Direct

Name: malS [H]

Synonym: ECH74115_4947

Alternate gene names: 209399658

Gene position: 4586684-4588714 (Clockwise)

Preceding gene: 209398178

Following gene: 209398430

Centisome position: 82.32

GC content: 52.78

Gene sequence:

>2031_bases
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTCCCTGC
CTTTAGCGAACAGGGAACGGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTCACGCTAAATTTTG
ACCAGCAGTGCTGGCAGCCTGCAGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCAAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCAT
CCAGAACGCCGCCGAACCGGTAGCAAACCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACGCTGGATGTCA
GCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAACG
TTACAACCCGCTGCTACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACGCCTCTGCCCCTTTCGACTGGCA
TAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAAAACGGCGATCCCAGTAATGACCAGAGTTACGGACGTCATA
AAGACGGTATGGCGGAAATTGGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAG
TTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTT
CCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTAC
GGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACG
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAAGTGAAAAAAACGCTGGGTGAACGCTG
GAGCGACTGGAAACCTGCCGCCGGGCAAACCTGGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGG
ATAAATGGTGGGGAAAAAACTGGATCCGTACCGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACCATGTCG
CTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAACGGATAC
CCACGCTAAAGCCATCGACGGCTTTACCCCTCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGA
TTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCTTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCG
CTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCA
CGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAG
CTGTCGATTGTATTGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGTCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGT
ACAAATCTTTTATGGCGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATA
TGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAACGTCGCGCACTGGCAGAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGCGGGCAAACAAACGACACTTTCGCTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAA
AGTGCTGGTCATCTGGGCTGGGCAACAGTGA

Upstream 100 bases:

>100_bases
CCTTGCTTCTCATCCTGTGGACTTACCGCTCAGGGATGAGTTTTGTCTGGCTTATCGCTGGCAAACTGTCTGAAATCGCA
GCAATAAGGACTCATCCACC

Downstream 100 bases:

>100_bases
CTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGA
TAAAGCACCGCTCACTCTTC

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGKYTLQIDTRSGTPTLMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAANVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Sequences:

>Translated_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGKYTLQIDTRSGTPTLMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAANVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ
>Mature_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGKYTLQIDTRSGTPTLMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAANVAHWQKISQFRARH
PAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=98.2248520710059, Blast_Score=1385, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=143, Percent_Identity=30.0699300699301, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75676; Mature: 75676

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPTLMISIQNAAEPVANLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHH
CPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDA
CCCCCCCEEEEEECCCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKAIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC
>Mature Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPTLMISIQNAAEPVANLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHH
CPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDA
CCCCCCCEEEEEECCCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKAIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]