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The map label for this gene is pcm [H]

Identifier: 209399278

GI number: 209399278

Start: 3693813

End: 3694439

Strand: Reverse

Name: pcm [H]

Synonym: ECH74115_3994

Alternate gene names: 209399278

Gene position: 3694439-3693813 (Counterclockwise)

Preceding gene: 209398515

Following gene: 209400494

Centisome position: 66.3

GC content: 54.07

Gene sequence:

>627_bases
ATGGTAAGCAGACGCGTACAAGCACTTCTGGATCAATTACGTGCGCAAGGTATTCAGGATGAGCAGGTGCTGAATGCACT
TGCCGCCGTGCCGCGTGAAAAATTCGTTGATGAAGCGTTTGAACAAAAAGCCTGGGACAATATCGCCTTGCCGATAGGTC
AGGGGCAGACGATTTCGCAGCCGTATATGGTGGCGCGAATGACCGAATTACTCGAGCTGACGCCGCAGTCGCGGGTGCTG
GAAATTGGCACCGGTTCGGGATATCAAACGGCAATCCTGGCGCATCTTGTCCAGCATGTTTGCTCGGTTGAACGGATTAA
AGGCTTGCAGTGGCAGGCGCGTCGCCGCCTGAAAAATCTTGATTTACATAATGTTTCAACCCGTCATGGCGATGGATGGC
AAGGTTGGCAGGCACGTGCGCCGTTTGACGCTATCATTGTTACGGCGGCACCGCCGGAAATTCCAACTGCGCTAATGACG
CAGCTGGACGAAGGCGGGATTCTCGTCTTACCCGTAGGGGAGGAGCACCAGTATTTGAAACGGGTGCGTCGTCGGGGAGG
CGAATTTATTATCGATACCGTGGAGGCCGTGCGCTTTGTCCCTTTAGTGAAGGGTGAGCTGGCTTAA

Upstream 100 bases:

>100_bases
AGGGCTATGTCTCCATCACGCCGCTGCATGTGGATTTAACTGCGCATAGCGCGCAAGATGTGGTTTCAGACTGGTTAAAC
AGCGTGGGAGTTGGCACGCA

Downstream 100 bases:

>100_bases
AACGTGAGGAAATACCTGGATTTTTCCTGGTTATTTTGCCGCAGGTCAGCGTATCGTGAACATCTTTTCCAGTGTTCAGT
ATGGTGCCTTGCACGGTAAT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQPYMVARMTELLELTPQSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA

Sequences:

>Translated_208_residues
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQPYMVARMTELLELTPQSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA
>Mature_208_residues
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQPYMVARMTELLELTPQSRVL
EIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=32.0754716981132, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1789100, Length=208, Percent_Identity=100, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71983477, Length=211, Percent_Identity=30.8056872037915, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=211, Percent_Identity=28.9099526066351, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17981723, Length=221, Percent_Identity=33.0316742081448, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23258; Mature: 23258

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQ
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNL
HHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMTQLDEGGILVLPVGEEHQYLK
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHH
RVRRRGGEFIIDTVEAVRFVPLVKGELA
HHHHCCCCEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQ
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNL
HHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMTQLDEGGILVLPVGEEHQYLK
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEECCCHHHHHH
RVRRRGGEFIIDTVEAVRFVPLVKGELA
HHHHCCCCEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA