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The map label for this gene is psuG [H]

Identifier: 209399243

GI number: 209399243

Start: 3033719

End: 3034657

Strand: Reverse

Name: psuG [H]

Synonym: ECH74115_3301

Alternate gene names: 209399243

Gene position: 3034657-3033719 (Counterclockwise)

Preceding gene: 209398647

Following gene: 209397477

Centisome position: 54.46

GC content: 49.09

Gene sequence:

>939_bases
ATGTCTGAATTAAAAATTTCCCCTGAATTATTACAAATTTCCCCGGAAGTGCAGGACGCTTTAAAAAACAAAAAACCGGT
TGTGGCGCTGGAATCGACCATTATTTCTCACGGGATGCCGTTCCCACAAAATGCCCAGACCGCAATTGAAGTAGAAGAAA
CTATTCGTAAACAGGGCGCAGTACCTGCCACTATCGCCATTATTGGCGGCGTGATGAAAGTGGGTTTAAGCAAAGAAGAA
ATTGAATTACTGGGTCGTGAAGGGCATAACGTGACTAAAGTTAGTCGTCGCGATTTACCTTTTGTCGTTGCCGCAGGAAA
AAATGGTGCAACCACCGTGGCTTCAACGATGATTATTGCGGCGCTTGCCGGAATTAAAGTATTTGCCACCGGCGGAATTG
GTGGTGTTCATCGAGGGGCGGAACATACCTTCGATATTTCTGCCGATTTGCAAGAACTGGCAAATACTAATGTCACCGTT
GTTTGTGCCGGGGCGAAATCTATTCTCGATTTAGGATTAACCACTGAGTATTTAGAAACCTTCGGTGTGCCGTTAATTGG
CTATCAGACTAAAGCGCTGCCTGCGTTTTTCTGCCGTACCAGCCCGTTTGACGTCAGCATTCGTCTCGACAGCGCCAGTG
AAATTGCCCGTGCAATGGCGGTGAAATGGCAAAGCGGGCTGAACGGTGGCCTCGTGGTAGCGAACCCGATCCCGGAACAG
TTTGCGATGCCAGAACACACTATCAATGCGGTGATCGATCAGGCGGTAGCTGAAGCTGAAGCGCAGGGTGTTATTGGTAA
AGAAAGTACGCCATTCCTGCTGGCGCGCGTTGCTGAGCTGACCGGCGGTGACAGCCTGAAATCCAACATCCAGCTTGTGT
TCAACAACGCCATTCTGGCGAGCGAAATTGCCAAAGAATATCAGCGTCTCGCGGGTTAA

Upstream 100 bases:

>100_bases
GTTTCGCACAGGGATGTTCGTCAATGGCGCTCTCCTGTGAATACACCAATAACCCCGATTTATCGATTGCCAACGTTATA
TCGTTAGTGGAGAACGCAGA

Downstream 100 bases:

>100_bases
TTTCTGACGGGCAGAAAGCCGTCGCCCGTACTCATCAAACATACCGAAACCTGGCGCTGACGCCGGGTTTACTGGCATGA
AGGGAAAATCATTATGGACA

Product: indigoidine synthase A like protein

Products: NA

Alternate protein names: PsiMP glycosidase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
FAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Sequences:

>Translated_312_residues
MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
FAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
>Mature_311_residues
SELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEI
ELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVV
CAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQF
AMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. In vitro, can also catalyze the reverse reaction [H]

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family [H]

Homologues:

Organism=Escherichia coli, GI1788490, Length=312, Percent_Identity=99.3589743589744, Blast_Score=626, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147470, Length=297, Percent_Identity=50.1683501683502, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24580669, Length=295, Percent_Identity=52.2033898305085, Blast_Score=287, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022830
- InterPro:   IPR007342 [H]

Pfam domain/function: PF04227 Indigoidine_A [H]

EC number: NA

Molecular weight: Translated: 32910; Mature: 32779

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGA
CCCCCCCHHHEEECHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHH
FGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
HCCCEECCCHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHH
FAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC
>Mature Secondary Structure 
SELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGA
CCCCCCHHHEEECHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHH
FGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
HCCCEECCCHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHH
FAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]