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The map label for this gene is psuK [H]

Identifier: 209398647

GI number: 209398647

Start: 3034645

End: 3035586

Strand: Reverse

Name: psuK [H]

Synonym: ECH74115_3302

Alternate gene names: 209398647

Gene position: 3035586-3034645 (Counterclockwise)

Preceding gene: 209399216

Following gene: 209399243

Centisome position: 54.48

GC content: 47.13

Gene sequence:

>942_bases
ATGCGCGAAAAGGATTATGTCGTAATTATAGGCTCGGCGAATATTGATGTCGCCGGATATTCACATGAATCATTAAATTA
TGCGGATTCAAATCCAGGTAAAATAAAATTTACGCCTGGTGGGGTAGGGCGCAATATTGCACAAAACCTGGCGTTGCTGG
GTAATAAAGCCTGGCTACTGAGCGCCGTAGGCAGTGATTTTTATGGCCAATCGCTGCTAACGCAAACCAATCAATCTGGC
GTTTATGTCGATAAATGCCTGATTGTGCCGGGAGAAAATACGTCGAGCTATTTATCATTACTCGATAATACCGGTGAAAT
GCTGGTTGCTATAAATGACATGAATATTAGCAACGCTATTACAGCTGAATATCTCGCACAGCACCGTGAATTTATTCAGA
GGGCAAAGGTCATTGTCGCGGACTGTAATATCAGTGAAGAGGCACTGGCATGGATTCTGGATAATGCTGCCAACGTACCC
GTATTTGTCGATCCGGTTTCCGCGTGGAAATGCGTCAAAGTACGCGAGCGATTAAGTCAAATCCATACTCTCAAGCCAAA
CCGCCTTGAAGCGGAAACCCTGAGTGGGATTGCGCTGTCAGGGCGTGAAGATGTGGCAAAAGTTGCTGCCTGGTTCCATC
AACATGGCCTGAACCGACTGGTATTGAGCATGGGCGGCGACGGCGTTTATTACAGCGATATCAGCGGTGAAAGTGGCTGG
TCTGCGCCGATCAAAACCAATGTTATTAATGTTACCGGAGCGGGCGATGCCATGATGGCGGGACTTGCTTCGTGTTGGGT
AGACGGAATGCCGTTTGCCGAATCTGTTCGTTTCGCACAGGGATGTTCGTCAATGGCGCTCTCCTGTGAATACACCAATA
ACCCCGATTTATCGATTGCCAACGTTATATCGTTAGTGGAGAACGCAGAATGTCTGAATTAA

Upstream 100 bases:

>100_bases
GAGCACATGTTTAAATATGAACACATGTTTAAACCGTCTTTGCAGTATCTATATCCTTGTGGATTGACAGGGTTGGTAAT
TTTTTACCAGAGGACGTGCT

Downstream 100 bases:

>100_bases
AAATTTCCCCTGAATTATTACAAATTTCCCCGGAAGTGCAGGACGCTTTAAAAAACAAAAAACCGGTTGTGGCGCTGGAA
TCGACCATTATTTCTCACGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN

Sequences:

>Translated_313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN
>Mature_313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=313, Percent_Identity=99.0415335463259, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1788484, Length=307, Percent_Identity=39.413680781759, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790193, Length=237, Percent_Identity=24.0506329113924, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17532905, Length=308, Percent_Identity=24.3506493506494, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580669, Length=281, Percent_Identity=30.6049822064057, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 33659; Mature: 33659

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEE
SAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAI
EECCCCHHCCHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCHHH
TAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRERLSQ
HHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHH
IHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
HHCCCCCCCCHHHHCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCC
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIA
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
NVISLVENAECLN
HHHHHHHHHHCCC
>Mature Secondary Structure
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEE
SAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAI
EECCCCHHCCHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCHHH
TAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRERLSQ
HHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHH
IHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
HHCCCCCCCCHHHHCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCC
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIA
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
NVISLVENAECLN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]