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The map label for this gene is priA [H]

Identifier: 209398783

GI number: 209398783

Start: 5029217

End: 5031415

Strand: Reverse

Name: priA [H]

Synonym: ECH74115_5393

Alternate gene names: 209398783

Gene position: 5031415-5029217 (Counterclockwise)

Preceding gene: 209397441

Following gene: 209397063

Centisome position: 90.3

GC content: 56.98

Gene sequence:

>2199_bases
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTCCTGCCAGAAGGCATGGCGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGACGTCAGTG
AGCTGCCGCTTAACGAGCTAAAAGCGGTAGTCGAAGTGCTGGATAGTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTG
CTGCTCTGGGCGGCGGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCATGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCACGCTTGAATTT
AATGATGCCGCGTTACAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CGGATACCTTCTCTGCTTGGCTGCTGGCGGGCGTTACAGGTTCCGGTAAAACAGAGGTTTATTTGAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTCATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGTGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACACCCGCACTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCGGGGCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGTCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTATTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGAGCGCTGGAACAACAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATATTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTTGCCCAGCTATACACCCAGGTAGCAGGCCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCATCATCCGGAACACCCGCTATTGCAAACGTTGCTCTATAAAGGCT
ACGACGCTTTTGCCGAACAGGCACTGGCTGAGCGGCGAATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAATTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAACGGTACGCTGGCACTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Upstream 100 bases:

>100_bases
GCGAAATCATACAGAAATTAACCAGCATATGCAAACTGATCCGCACTCTTCTACGGCAATGTGTATACTAACCCACCGAA
TTTCAAGTCAGGATGATGCT

Downstream 100 bases:

>100_bases
ACCGCTCACGATGCGAGGCGGATCGAAAAATTCAATATTCATCACACTTTTCATGAAAATTCTGTAACCGTTTTCACGCG
CTATCTGCTAAAAATGTTGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Sequences:

>Translated_732_residues
MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG
>Mature_731_residues
PVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLL
LWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEFN
DAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLENV
LAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDE
EHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQA
GLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCGS
THLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVRA
EDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKV
KWVLDVDPIEG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=99.1803278688525, Blast_Score=1489, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81626; Mature: 81495

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNEL
CCCCEEEECCCCCCCHHHHCCCCCEEECCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEFNDAALQALRKKGLCDLASET
CCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHCCCHHHHCCC
PEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
CCCCCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQAGLAPALITRMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQ
CCEEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHCHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCC
VKWVLDVDPIEG
EEEEEECCCCCC
>Mature Secondary Structure 
PVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNEL
CCCEEEECCCCCCCHHHHCCCCCEEECCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEFNDAALQALRKKGLCDLASET
CCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHCCCHHHHCCC
PEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
CCCCCCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCCHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEEECCCCCCEEEECCCHH
LETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQAGLAPALITRMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQ
CCEEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHCHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLI
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCC
VKWVLDVDPIEG
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]