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The map label for this gene is cytR

Identifier: 209397063

GI number: 209397063

Start: 5028036

End: 5029028

Strand: Reverse

Name: cytR

Synonym: ECH74115_5391

Alternate gene names: 209397063

Gene position: 5029028-5028036 (Counterclockwise)

Preceding gene: 209398783

Following gene: 209400145

Centisome position: 90.25

GC content: 51.76

Gene sequence:

>993_bases
ATGAAAGACGTTGCCCTCAAGGCAAAAGTCTCTACAGCGACCGTCTCCCGAGCATTAATGAATCCCGATAAAGTCTCCCA
AGCCACCCGTAATCGGGTTGAAAAAGCGGCCCGTGAAGTGGGTTATTTACCGCAGCCTATGGGGCGCAACGTCAAGCGTA
ATGAATCCCGCACCATTCTGGTGATTGTCCCGGATATCTGCGATCCCTTCTTTAGCGAAATTATTCGCGGTATCGAAGTT
ACGGCGGCAAATCACGGATATCTGGTGCTGATTGGCGACTGTGCGCATCAAAATCAGCAGGAAAAAACCTTTATCGATTT
GATCATCACCAAGCAAATTGATGGCATGTTGCTGCTGGGTTCAAGGCTGCCGTTTGATGCCAGCATTGAGGAACAGCGTA
ATCTGCCGCCGATGGTGATGGCGAACGAATTTGCACCGGAACTGGAGCTGCCTACCGTTCATATCGACAATCTGACCGCC
GCATTTGATGCAGTAAATTATTTATATGAGCAAGGGCATAAACGGATTGGCTGTATAGCCGGTCCCGAAGAGATGCCGCT
GTGTCACTACCGCCTGCAAGGTTACGTTCAGGCGCTGCGTCGCTGCGGCATTATGGTTGATCCGCAATACATCGCCCGTG
GCGACTTCACCTTCGAAGCCGGAAGCAAAGCGATGCAGCAGCTGCTTGATCTTCCACAACCGCCTACTGCTGTCTTCTGC
CATAGCGATGTGATGGCGCTCGGCGCACTTTCTCAGGCAAAACGCCAGGGGCTGAAAGTCCCGGAAGACCTTTCCATAAT
CGGTTTTGATAACATCGACCTGACGCAATTTTGTGATCCGCCGCTGACAACCATCGCGCAGCCGCGTTACGAAATCGGTC
GGGAAGCTATGCTGTTATTGCTTGATCAAATGCAGGGGCAACACGTTGGCAGTGGCTCTCGTTTAATGGACTGCGAACTT
ATCATCCGGGGATCAACACGCGCGTTACCTTAA

Upstream 100 bases:

>100_bases
TAAAAATGTTGCCGATGTGAAGTAAACATGGATGTAGTACGCCTGACGTGCCAGGCGAGGAGTGAGTGTGAAAGCGAAGA
AGCAGGAAACTGCCGCGACC

Downstream 100 bases:

>100_bases
AGTAAACCTTTAAGACTGCCGTGACTGGTCAAAGGCCCGCCGCTTAAGTAACATGGCGGGCTGACGAACGAATAAATACA
GCGAAACGATAGTGGCACAA

Product: DNA-binding transcriptional regulator CytR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEV
TAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTA
AFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFC
HSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCEL
IIRGSTRALP

Sequences:

>Translated_330_residues
MKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEV
TAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTA
AFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFC
HSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCEL
IIRGSTRALP
>Mature_330_residues
MKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEV
TAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTA
AFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFC
HSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCEL
IIRGSTRALP

Specific function: This protein negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins. Binds cytidine and adenosine as effectors

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=100, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=307, Percent_Identity=36.8078175895765, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=35.1791530944625, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=34.9673202614379, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=33.2179930795848, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=30.3225806451613, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=29.2604501607717, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=27.4760383386581, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=26.6025641025641, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=27.5449101796407, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787906, Length=315, Percent_Identity=26.031746031746, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=26.6272189349112, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=23.0769230769231, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=24.8407643312102, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYTR_ECO57 (P0ACN9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E86083
- PIR:   E91236
- RefSeq:   NP_290565.1
- RefSeq:   NP_312888.1
- ProteinModelPortal:   P0ACN9
- SMR:   P0ACN9
- EnsemblBacteria:   EBESCT00000023892
- EnsemblBacteria:   EBESCT00000057373
- GeneID:   915035
- GeneID:   960203
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5481
- KEGG:   ecs:ECs4861
- GeneTree:   EBGT00050000009237
- HOGENOM:   HBG753640
- OMA:   FEAVHYL
- ProtClustDB:   PRK11041
- BioCyc:   ECOL83334:ECS4861-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36632; Mature: 36632

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTIL
CCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLG
EEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCEEEEC
SRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNYLYEQGHKRIGCIA
CCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEC
GPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFC
CCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHHHHCCCCCCCEEEE
HSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLL
CCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHH
LDQMQGQHVGSGSRLMDCELIIRGSTRALP
HHHHCCCCCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTIL
CCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLG
EEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHEEEEEEECCCCCEEEEC
SRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNYLYEQGHKRIGCIA
CCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEC
GPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFC
CCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHHHHCCCCCCCEEEE
HSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLL
CCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHH
LDQMQGQHVGSGSRLMDCELIIRGSTRALP
HHHHCCCCCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796