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The map label for this gene is pcaK [H]

Identifier: 209398712

GI number: 209398712

Start: 3004456

End: 3005814

Strand: Reverse

Name: pcaK [H]

Synonym: ECH74115_3271

Alternate gene names: 209398712

Gene position: 3005814-3004456 (Counterclockwise)

Preceding gene: 209397480

Following gene: 209399809

Centisome position: 53.94

GC content: 56.95

Gene sequence:

>1359_bases
ATGACTCAACGACGTGATTTACAAGCACTTATCGATGCTGCTCCGGTCGGCAAGATGCAGTGGCGCGTTATCATCTGCTG
TTTTCTGGTGGTAATGCTCGATGGTTTTGACACCGCGGCTATCGGATTTATCGCCCCTGATATTCGCACTCACTGGCAAC
TCACCGCAGGCGATCTCGCCCCGCTTTTTGGCGCGGGTCTGCTAGGATTAACCGCAGGCGCACTGCTTTGTGGCCCGTTG
TCCGATCGCTTTGGTCGCAAGCGGGTGATTGAGCTGTGCGTCTTTTTGTTTGGCGCGTTAAGCCTCGCCTCGGCTTTCTC
ACCGGATCTGCAAACGCTGGTCTTCCTGCGCTTTCTCACCGGCCTGGGTCTGGGCGGCGCGATGCCGAACACCATCACCA
TGACCTCGGAGTATCTGCCTGCCCGCCGCCGCGGCGCGCTGGTGACGCTGATGTTTTGCGGCTTTACGCTGGGTTCGGCG
TTTGGCGGGATTGTCAGTGCGCAGCTGGTGCCGGTGATTGGCTGGCACGGCATTCTGGTGTTGGGGGGCGTGTTACCGCT
GATGCTGTTTGTCGCACTGCTGGTAGTTCTGCCGGAATCGCCACGCTGGCAGGTGCGCCGCCAGCTACCGCAGGCGGTGA
TTGCGAAAACCGTCAGCGCCATCACCCGCGAGCGTTATGTCGATACCCACTTTTATCTCATTGAATCAGCCTCCGTTACA
AAAGGCAGCATTCGTCAACTGTTTATGGGACGTCAGTTACCCATCACGTTGATGCTGTGGGTAGTGTTCTTTATGAGCCT
GCTGATTATTTACCTGCTCTCAAGCTGGATGCCGACGCTACTGAACCATCGCGGCATCGACCTGCAACACGCGTCCAGGG
TCACCGCCGCGTTCCAGATTGGTGGTACGTTAGGCGCGCTGGCGCTGGGTGTGCTGATGGATAAGTTCAATCCTTTCCGC
GTGCTGACGCTGAGCTATGCCATAGGCGCTATATGCATCGTGATGATTGGCTTAAGCCAGGATGGATTATGGTTGATGGC
GCTGGCGATTTTCGGCACTGGCATTGGCATTAGCGGATCGCAGGTGGGGCTGAATGCGCTGACCGCAACACTTTATCCAA
CACAAAGTCGAGCCACGGGCGTGAGCTGGTCAAATGCTATCGGCCGCTGCGGCGCGATTGTTGGTTCGCTGTCTGGCGGC
GTGATGATGGCGATGAATTTCTCTTTCGACACCCTGTTTTTCATTATCGCTGTGCCTGCGGCGATCAGTGCGGTGATGTT
AACCCTGCTGATTACCGTGGTTCGCCAGTCGACCTCTGTCCCTGACTCACTGCCTCGCGCAGGCGTTGTGAACGAATAA

Upstream 100 bases:

>100_bases
TGCCCGAAATGCCATTGTACATCTCATCTCTTTAATCACAATATCGCAACATATACAAAACAAAAGAAACATCTACCTTA
CCTGCACTGAGGCATTTACT

Downstream 100 bases:

>100_bases
GGAGCATCTCAATGACTGATAACAATCAAAATAGCCGCGAGCAATTTTATCAGCATATTTCCGGGCAAAACCTGACCCCG
CTGTGGGAGTCACTGCACCA

Product: transporter, major facilitator family

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPL
SDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFR
VLTLSYAIGAICIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGG
VMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVVNE

Sequences:

>Translated_452_residues
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPL
SDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFR
VLTLSYAIGAICIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGG
VMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVVNE
>Mature_451_residues
TQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLS
DRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSAF
GGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVTK
GSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASRVTAAFQIGGTLGALALGVLMDKFNPFRV
LTLSYAIGAICIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGGV
MMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVVNE

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=418, Percent_Identity=25.8373205741627, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI213021148, Length=449, Percent_Identity=26.5033407572383, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI7662270, Length=343, Percent_Identity=24.4897959183673, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI216548223, Length=337, Percent_Identity=24.0356083086053, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI262399383, Length=280, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI166064021, Length=340, Percent_Identity=26.7647058823529, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI73695465, Length=336, Percent_Identity=24.4047619047619, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4507005, Length=380, Percent_Identity=25.2631578947368, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI24497476, Length=393, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI20070188, Length=393, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI8923870, Length=338, Percent_Identity=24.2603550295858, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87081723, Length=385, Percent_Identity=36.8831168831169, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082231, Length=420, Percent_Identity=23.0952380952381, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788068, Length=465, Percent_Identity=24.0860215053763, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082159, Length=396, Percent_Identity=27.7777777777778, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786229, Length=368, Percent_Identity=24.4565217391304, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788074, Length=351, Percent_Identity=23.6467236467236, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=20.9424083769633, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789152, Length=450, Percent_Identity=21.7777777777778, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789207, Length=178, Percent_Identity=30.3370786516854, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=404, Percent_Identity=23.7623762376238, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320595, Length=141, Percent_Identity=30.4964539007092, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922874, Length=407, Percent_Identity=23.5872235872236, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24640198, Length=459, Percent_Identity=23.3115468409586, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640196, Length=459, Percent_Identity=23.3115468409586, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24640200, Length=459, Percent_Identity=23.3115468409586, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI161077756, Length=499, Percent_Identity=21.8436873747495, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48430; Mature: 48299

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCEEECCCCHH
PLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSAFGGIVSAQLVPVIGWHGILV
HCCCCCCCCCCEEECHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASRVTAAFQI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GGTLGALALGVLMDKFNPFRVLTLSYAIGAICIVMIGLSQDGLWLMALAIFGTGIGISGS
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
QVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGGVMMAMNFSFDTLFFIIAVPA
CCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
AISAVMLTLLITVVRQSTSVPDSLPRAGVVNE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCEEECCCCHH
PLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSAFGGIVSAQLVPVIGWHGILV
HCCCCCCCCCCEEECHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASRVTAAFQI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GGTLGALALGVLMDKFNPFRVLTLSYAIGAICIVMIGLSQDGLWLMALAIFGTGIGISGS
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
QVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGGVMMAMNFSFDTLFFIIAVPA
CCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
AISAVMLTLLITVVRQSTSVPDSLPRAGVVNE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]