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The map label for this gene is lldP

Identifier: 209398657

GI number: 209398657

Start: 4627347

End: 4629002

Strand: Direct

Name: lldP

Synonym: ECH74115_4976

Alternate gene names: 209398657

Gene position: 4627347-4629002 (Clockwise)

Preceding gene: 209397689

Following gene: 209395948

Centisome position: 83.05

GC content: 55.07

Gene sequence:

>1656_bases
ATGAATCTCTGGCAACAAAACTACGATCCCGCCGGGAATATCTGGCTTTCCAGTTTGATAGCATCGCTTCCCATCCTGTT
TTTCTTCTTTGCGCTGATTAAGCTCAAACTGAAAGGATACGTCGCCGCCTCATGGACGGTAGCAATCGCCCTTGCCGTGG
CTTTGCTGTTCTATAAAATGCCGGTCGCTAACGCGCTGGCCTCGGTGGTTTATGGCTTCTTCTATGGTTTGTGGCCCATC
GCGTGGATCATTATTGCAGCAGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTAT
TCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTTTCTGTTTCGGCGCTTTCCTTGAAGGAGCCGCAG
GCTTTGGCGCACCGGTAGCAATTACCGCCGCATTGCTGGTCGGCCTGGGTTTTAAACCGCTGTACGCCGCCGGACTGTGC
CTGATTGTTAACACCGCGCCAGTGGCATTTGGCGCGATGGGCATTCCAATCCTGGTTGCCGGACAAGTAACAGGCATCGA
CAGCTTTGAGATTGGCCAGATGGTGGGGCGGCAGCTACCGTTTATGACCATTATCGTGCTGTTCTGGATCATGGCGATTA
TGGACGGCTGGCGCGGTATCAAGGAGACGTGGCCTGCGGTCGTGGTTGCGGGCGGCTCGTTTGCCATCGCTCAGTACCTT
AGCTCTAACTTCATTGGGCCGGAGCTGCCGGACATTATCTCTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAA
ACGCTGGCAGCCAGTGCGCGTATTCCGTTTTGGTGATTTGGGGGCGTCACAGGTTGATATGACGCTAGCCCACACCGGTT
ACACTGCGGGTCAGGTGTTACGTGCCTGGACACCGTTCCTGTTCCTGACAGCTACCGTAACACTGTGGAGTATCCCGCCG
TTTAAAGCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAACATTCCGGTGCCGTACCTCGATAAACTGGT
TGCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGTTCTCTGCCACCGGCA
CCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGC
ACGCTGAAAGAACTGGCTCTACCCATCTACTCCATCGGCATGGTGCTGGCGTTCGCCTTTATCTCGAACTATTCCGGACT
GTCATCAACACTGGCACTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGG
TATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCACCGCAGCACAACAAATTGGCGTC
TCTGATCTGTTGCTGGTTGCCGCCAATACCACCGGTGGCGTCACCGGTAAGATGATCTCCCCGCAATCTATCGCTATCGC
CTGTGCGGCGGTAGGCCTGGTGGGCAAAGAGTCTGATTTGTTCCGCTTTACTGTCAAACACAGCCTGATCTTCACCTGTA
TGGTGGGCGTGATCACCACGCTTCAGGCTTATGTCTTAACGTGGATGATTCCTTAA

Upstream 100 bases:

>100_bases
CTACACAACACAATTGGCAGTGCCACTTTTACACAACGTGTGACAAGGAGATGAGCAACAGACTCATTACACGATGTGCG
TGGACTCCAGGAGACCTGCA

Downstream 100 bases:

>100_bases
TGATTGTTTTACCCAGACGCCTGTCAGACGAGGTTGCCGATCGTGTGCGGGCGCTGATTGATGAAAAAAACCTGGAAGCG
GGCATGAAGTTGCCCGCTGA

Product: L-lactate permease

Products: Proton [Cytoplasm]; lactate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKMPVANALASVVYGFFYGLWPI
AWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPP
FKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGS
TLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP

Sequences:

>Translated_551_residues
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKMPVANALASVVYGFFYGLWPI
AWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPP
FKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGS
TLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP
>Mature_551_residues
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKMPVANALASVVYGFFYGLWPI
AWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPP
FKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGS
TLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP

Specific function: Transports L-lactate across the membrane. Can also transport D-lactate and glycolate. Seems to be driven by a proton motive force

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family

Homologues:

Organism=Escherichia coli, GI1790031, Length=551, Percent_Identity=99.8185117967332, Blast_Score=1087, Evalue=0.0,
Organism=Escherichia coli, GI1789347, Length=558, Percent_Identity=63.0824372759857, Blast_Score=685, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LLDP_ECO57 (P65254)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98189
- PIR:   B86036
- RefSeq:   NP_290186.1
- RefSeq:   NP_312508.1
- ProteinModelPortal:   P65254
- EnsemblBacteria:   EBESCT00000025590
- EnsemblBacteria:   EBESCT00000060370
- GeneID:   915577
- GeneID:   960946
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5030
- KEGG:   ecs:ECs4481
- GeneTree:   EBGT00050000008813
- HOGENOM:   HBG289840
- OMA:   WSIKPFK
- ProtClustDB:   PRK10420
- BioCyc:   ECOL83334:ECS4481-MONOMER
- InterPro:   IPR003804
- TIGRFAMs:   TIGR00795

Pfam domain/function: PF02652 Lactate_perm

EC number: NA

Molecular weight: Translated: 59187; Mature: 59187

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x127f7504)-; HASH(0x121dba78)-; HASH(0xf4f948c)-; HASH(0x12686db8)-; HASH(0x111c8608)-; HASH(0x128e9a94)-; HASH(0x11d5262c)-; HASH(0x128cebec)-; HASH(0x105c8f94)-; HASH(0x11a4762c)-; HASH(0x1092ef48)-; HASH(0x11f1c868)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHH
VGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLCLIVNTAPVAFGAMGIPILVA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHCCCCCCEEEE
GQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCHHHHHHHH
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVL
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHEEEHEECCCHHHHHH
RAWTPFLFLTATVTLWSIPPFKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAY
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEHEEECCHHHHHHHHHHCCCHHHHHHHH
AAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGSTLKELALPIYSIGMVLAFAF
HHHHEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCC
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITT
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
LQAYVLTWMIP
HHHHHHHHCCH
>Mature Secondary Structure
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHH
VGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLCLIVNTAPVAFGAMGIPILVA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEECCCHHHCCCCCCEEEE
GQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCHHHHHHHH
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVL
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHEEEHEECCCHHHHHH
RAWTPFLFLTATVTLWSIPPFKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAY
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEHEEECCHHHHHHHHHHCCCHHHHHHHH
AAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGSTLKELALPIYSIGMVLAFAF
HHHHEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
HHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCC
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCMVGVITT
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
LQAYVLTWMIP
HHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796