BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is caiD [H]

Identifier: 209398425

GI number: 209398425

Start: 39784

End: 40677

Strand: Reverse

Name: caiD [H]

Synonym: ECH74115_0040

Alternate gene names: 209398425

Gene position: 40677-39784 (Counterclockwise)

Preceding gene: 209400477

Following gene: 209396400

Centisome position: 0.73

GC content: 52.46

Gene sequence:

>894_bases
ATGAAACGGCAGGGAACTACCCTGCCAGCTAATAATCACGCTCTAAAACAATATGCATTTTTTGCAGGGATGTTGTCGTC
CCTGAAAAAGCAAAAATGGAGAAAAGGAATGAGTGAATCATTACATCTGACCCGCAATGGATCAATTCTGGAAATTACTC
TTGATCGCCCAAAAGCGAATGCTATTGATGCAAAAACCAGCTTTGAAATGGGCGAAGTATTTCTAAATTTCCGTGACGAT
CCGCAATTACGTGTCGCCATTATTACCGGTGCCGGAGAGAAATTCTTTTCCGCGGGCTGGGATTTAAAAGCGGCAGCAGA
AGGCGAAGCACCGGATGCTGACTTTGGCCCGGGTGGTTTTGCGGGATTAACCGAAATTTTCAATCTCGACAAACCGGTTA
TCGCAGCTGTGAACGGCTACGCCTTTGGCGGCGGTTTCGAACTGGCGCTGGCGGCAGATTTTATTGTTTGTGCCGATAAC
GCCAGCTTCGCCCTGCCGGAAGCCAAACTGGGCATCGTTCCTGACAGCGGCGGTGTGCTGCGTCTGCCGAAGATCCTGCC
GCCTGCCATCGTCAATGAAATGGTGATGACCGGCAGACGAATGGGCGCAGAAGAGGCGCTGCGTTGGGGGATAGTCAACC
GCGTGGTTAGCCAGGCGGAACTGATGGATAACGCCCGCGAACTGGCTCAGCAGCTGGTTAACAGCGCCCCGCTGGCGATT
GCGGCGCTGAAAGAGATCTACCGCACCACCAGCGAAATGCCGGTAGAAGAATCGTATAGCTATATTCGCAGCGGCGTGTT
GAAACACTATCCATCGGTTCTGCATTCGGAAGATGCCATTGAAGGGCCGCTGGCGTTTGCCGAGAAGCGCGATCCGGTGT
GGAAAGGACGTTAA

Upstream 100 bases:

>100_bases
ACAAAATATGGCGAAATTTAAAGTGCCCTCTTATCTGGAGATCAGAAAAGATCTGCCACGTAATTGCTCGGGGAAAATAA
TTAGAAAGAATCTGAAATAA

Downstream 100 bases:

>100_bases
CAACCGTGAGCTATTACGCCTTTGAGGGGTTAATTCCGGTGGTTCACCCGACGGCGTTTGTCCATCCCAGTGCCGTCTTG
ATTGGCGATGTGATTGTGGG

Product: carnitinyl-CoA dehydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN
ASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI
AALKEIYRTTSEMPVEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR

Sequences:

>Translated_297_residues
MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN
ASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI
AALKEIYRTTSEMPVEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR
>Mature_297_residues
MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN
ASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI
AALKEIYRTTSEMPVEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=34.2007434944238, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=30.8, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=34.2245989304813, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594471, Length=241, Percent_Identity=29.4605809128631, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI37594469, Length=227, Percent_Identity=29.5154185022026, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=29.9212598425197, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4502327, Length=225, Percent_Identity=32.4444444444444, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=28, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=28, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=28, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=27.1676300578035, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=136, Percent_Identity=27.9411764705882, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694520, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=99.2337164750958, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=35.3535353535354, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=32.1100917431193, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=29.8850574712644, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=29.4354838709677, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=165, Percent_Identity=33.9393939393939, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=34.1085271317829, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=28.9156626506024, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=31.8181818181818, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=25.6603773584906, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=30.0578034682081, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=28.1818181818182, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.0632411067194, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=29.5698924731183, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=182, Percent_Identity=26.3736263736264, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=35, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=35, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=35.2941176470588, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922422, Length=287, Percent_Identity=28.9198606271777, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=30.9090909090909, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=28.2608695652174, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571730, Length=249, Percent_Identity=27.710843373494, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571729, Length=249, Percent_Identity=27.710843373494, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583077, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583079, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 32256; Mature: 32256

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKAN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCEEEEEECCCCCC
AIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGF
CCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCH
AGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVL
HHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEEEECCCCEECCCCCEEEECCCCCEE
RLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI
ECCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AALKEIYRTTSEMPVEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKAN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCEEEEEECCCCCC
AIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGF
CCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCH
AGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVL
HHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEEEECCCCEECCCCCEEEECCCCCEE
RLPKILPPAIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI
ECCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AALKEIYRTTSEMPVEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA