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The map label for this gene is gltK

Identifier: 209397952

GI number: 209397952

Start: 769890

End: 770564

Strand: Reverse

Name: gltK

Synonym: ECH74115_0745

Alternate gene names: 209397952

Gene position: 770564-769890 (Counterclockwise)

Preceding gene: 209397185

Following gene: 209395778

Centisome position: 13.83

GC content: 50.81

Gene sequence:

>675_bases
ATGTACGAGTTTGACTGGAGTTCCATTGTCCCTTCCCTGCCATATCTGCTCGACGGGCTGGTGATCACGCTGAAAATCAC
CGTCACGGCAGTCGTGATTGGTATTTTGTGGGGCACCATGCTGGCGGTGATGCGCTTGTCCAGCTTTGCGCCCGTAGCGT
GGTTTGCCAAAGCCTACGTTAACGTATTCCGCTCGATTCCTTTAGTTATGGTTTTGCTGTGGTTTTACCTGATCGTGCCG
GGTTTTCTGCAAAACGTGCTGGGATTATCACCAAAAAATGATATCCGTTTAATTTCGGCGATGGTGGCGTTTTCGATGTT
TGAAGCGGCCTACTATTCAGAGATTATCCGTGCCGGTATTCAAAGTATTTCTCGTGGTCAGTCGAGCGCCGCGCTGGCGT
TGGGAATGACTCACTGGCAGTCGATGAAACTGATTATTCTGCCGCAGGCGTTCCGCGCGATGGTGCCGCTGCTGCTCACT
CAGGGCATCGTACTGTTCCAGGATACCTCGCTGGTGTATGTGTTATCTCTGGCCGATTTCTTCCGTACCGCCTCAACCAT
TGGTGAGCGTGATGGCACGCAGGTTGAGATGATCCTGTTTGCCGGATTTGTTTATTTCGTTATTAGCCTTAGCGCGTCGT
TGTTGGTCAGCTACTTGAAAAGAAGGACAGCATAA

Upstream 100 bases:

>100_bases
CCATTACGCTAGCGTATGTGTTTATTAACGCTTTTATCATGCTGGTGATGACGCTGGTTGAACGTAAAGTTCGCCTGCCT
GGCAACATGGGGGGCAAATA

Downstream 100 bases:

>100_bases
TGATTACCCTGAAAAATGTTTCAAAATGGTATGGTCACTTTCAGGTGCTGACCGACTGCTCAACCGAAGTGAAAAAAGGC
GAAGTGGTGGTGGTTTGCGG

Product: glutamate/aspartate ABC transporter, permease protein GltK

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Sequences:

>Translated_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
>Mature_224_residues
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVP
GFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=100, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=37.3831775700935, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=31.7307692307692, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1787030, Length=227, Percent_Identity=30.8370044052863, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786874, Length=215, Percent_Identity=26.046511627907, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787087, Length=217, Percent_Identity=27.1889400921659, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI48994930, Length=130, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788646, Length=210, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788645, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLTK_ECO57 (P0AER7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90715
- PIR:   G85565
- RefSeq:   NP_286379.1
- RefSeq:   NP_308719.1
- ProteinModelPortal:   P0AER7
- EnsemblBacteria:   EBESCT00000025465
- EnsemblBacteria:   EBESCT00000059557
- GeneID:   917053
- GeneID:   957713
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0803
- KEGG:   ecs:ECs0692
- GeneTree:   EBGT00050000008884
- HOGENOM:   HBG704821
- OMA:   PSMPYLL
- ProtClustDB:   PRK15107
- BioCyc:   ECOL83334:ECS0692-MONOMER
- InterPro:   IPR010065
- InterPro:   IPR000515
- TIGRFAMs:   TIGR01726

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 24915; Mature: 24915

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x252a0d98)-; HASH(0x2358d0f0)-; HASH(0x25647180)-; HASH(0x239c9948)-; HASH(0x25c2b138)-; HASH(0x2557aee8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYV
CCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF
HHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHH
FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSYLKRRTA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796