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The map label for this gene is gltJ [H]

Identifier: 209397185

GI number: 209397185

Start: 770564

End: 771304

Strand: Reverse

Name: gltJ [H]

Synonym: ECH74115_0746

Alternate gene names: 209397185

Gene position: 771304-770564 (Counterclockwise)

Preceding gene: 209399444

Following gene: 209397952

Centisome position: 13.84

GC content: 52.36

Gene sequence:

>741_bases
ATGTCTATAGACTGGAACTGGGGTATTTTTTTACAACAAGCCCCGTTCGGCAACACCACCTATCTTGGTTGGATCTGGAG
TGGTTTTCAGGTGACGATCGCTTTGTCGATCTGCGCCTGGATTATCGCTTTCCTCGTCGGTTCATTTTTCGGTATTTTAC
GTACCGTTCCAAACCGTTTTCTCTCCGGTCTGGGTACGTTGTATGTCGAACTGTTCCGCAACGTGCCGTTGATTGTGCAA
TTCTTTACTTGGTATCTGGTGATCCCGGAGCTGCTGCCGGAGAAAATCGGCATGTGGTTTAAGGCCGAGCTGGATCCCAA
TATTCAGTTTTTCCTTTCATCCATGCTCTGCCTGGGGCTGTTTACCGCCGCCCGTGTTTGCGAACAGGTACGCGCCGCGA
TTCAGTCGCTGCCGCGTGGGCAGAAGAATGCCGCGCTGGCTATGGGTCTGACGCTGCCGCAGGCTTACCGCTACGTACTG
CTGCCTAATGCTTATCGCGTTATCGTCCCGCCGATGACCTCAGAGATGATGAACCTGGTGAAAAACTCCGCCATCGCCTC
GACCATCGGTCTGGTAGATATGGCAGCGCAGGCGAGTAAATTGCTCGATTACTCGGCTCACGCCTGGGAGTCGTTTACCG
CCATTACGCTAGCGTATGTGTTTATTAACGCTTTTATCATGCTGGTGATGACGCTGGTTGAACGTAAAGTTCGCCTGCCT
GGCAACATGGGGGGCAAATAA

Upstream 100 bases:

>100_bases
ATGGTCGTTCCCCATCGGGCCTGAAAACCGCAATACGCTGGGTAACAATCTTCGAGGGTAGCAGTTAACGCTGCTACCCT
CTTTTTTCTGGAGTAGATTT

Downstream 100 bases:

>100_bases
TGTACGAGTTTGACTGGAGTTCCATTGTCCCTTCCCTGCCATATCTGCTCGACGGGCTGGTGATCACGCTGAAAATCACC
GTCACGGCAGTCGTGATTGG

Product: glutamate/aspartate ABC transporter, permease protein GltJ

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLAYVFINAFIMLVMTLVERKVRLP
GNMGGK

Sequences:

>Translated_246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLAYVFINAFIMLVMTLVERKVRLP
GNMGGK
>Mature_245_residues
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQF
FTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLL
PNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLAYVFINAFIMLVMTLVERKVRLPG
NMGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=99.1869918699187, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1788226, Length=198, Percent_Identity=32.8282828282828, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786873, Length=215, Percent_Identity=26.5116279069767, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI48994930, Length=123, Percent_Identity=39.0243902439024, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787030, Length=213, Percent_Identity=23.4741784037559, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=27.1889400921659, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787086, Length=218, Percent_Identity=27.0642201834862, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788646, Length=223, Percent_Identity=28.6995515695067, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082239, Length=175, Percent_Identity=24.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787087, Length=222, Percent_Identity=23.8738738738739, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27567; Mature: 27436

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF
CCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLAYVFINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCC
>Mature Secondary Structure 
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF
CCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLAYVFINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]