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The map label for this gene is hypF [H]

Identifier: 209397726

GI number: 209397726

Start: 3663337

End: 3665637

Strand: Reverse

Name: hypF [H]

Synonym: ECH74115_3962

Alternate gene names: 209397726

Gene position: 3665637-3663337 (Counterclockwise)

Preceding gene: 209397141

Following gene: 209399708

Centisome position: 65.79

GC content: 57.54

Gene sequence:

>2301_bases
GTGTCCGGCTGGACGGGTTACGCAATTCATGATGGCGGGAATAACTCAATGGCAAAAAACACATCTTGCGGTGTCCAACT
GCGTATTCGAGGCAAAGTGCAGGGCGTCGGTTTTCGTCCGTTTGTCTGGCAGCTGGCACAGCAATTAAATCTTCACGGCG
ATGTCTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCTGGAAGACCCGGAAACGTTTCTTGTTCAATTGCATCAGCAC
TGCCCGCCGCTGGCGCGTATTGATAGCGTTGAGCGTGAACCGTTTATCTGGTCACAACTGCCCACTGAGTTCACCATCCG
CCAGAGCGCGGGCGGCGCCATGAATACGCAAATTGTTCCCGATGCCGCCACTTGCCCTGCTTGCCTTGCCGAAATGAATA
CCCCAGGCGAACGGCGTTATCGTTATCCATTTATCAACTGTACCCACTGCGGCCCGCGCTTCACCATTATTCGCGCCATG
CCTTACGATCGCCCGTTTACCGTGATGGCGGCGTTTCCGCTGTGTCCAGCCTGTGATAAAGAGTACCGCGACCCGCTCGA
TCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCGTATCTTGAATGGGTAAGTCATGGTGAACATGCAG
AACAAGAGGCGGCATTACAGGCGGCTATCGCACAGTTAAAAATGGGCAACATTGTCGCCATCAAAGGGATTGGCGGATTT
CATCTTGCCTGCGATGCACGTAACAGTAACGCGGTGGCGACACTTCGGGCGCGCAAACATCGCCCGGCGAAACCGCTGGC
AGTCATGTTGCCAGTGGCTGACGGTTTACCAGACGCTGCGCGCCAGTTGCTTACCACGCCCGCCGCGCCGATTGTGCTGG
TGGATAAAAAATACGTTCCTGAGCTTTGTGATGATATCGCCCCTGGCCTTAACGAAGTCGGGGTAATGTTGCCTGCGAAT
CCGCTTCAGCATTTGCTGTTACAGGAACTACAATGCCCGCTGGTGATGACCTCTGGCAACCTGAGCGGTAAACCACCGGC
TATCAGCAACGAACAGGCGCTGGAGGATTTGCAGGGCATTGCCGACGGATTCTTAATACACAACCGCGACATTGTGCAGC
GGATGGATGATTCGGTGGTGCGCGAAAGCGGCGAAATGCTGCGCCGTTCGCGGGGGTATGTGCCGGATGCGCTGGCTTTG
CCTCCGGGCTTTAAAAATGTTCCGCCTGTACTGTGTCTCGGCGCGGATCTGAAAAATACCTTCTGCCTGGTGCGCGGTGA
ACAAGTGGTGTTGAGCCAGCATCTGGGCGATTTAAGTGACGATGGCATCCAGACGCAGTGGCGCGAAGCGTTACGCCTGA
TGCAAAACATCTACAATTTCACTCCGCAATACGTTGTGCATGACGCACATCCGGGCTATGTCTCCTGCCAGTGGGCGAGC
GAAATGAATCTGCCGACGCAAACGGTGCTGCATCATCATGCCCACGCAGCGGCGTGTCTGGCAGAGCATCAGTGGCCGCT
GGATGGCGGTGATGTCATTGCTTTGACGCTCGACGGTATCGGTATGGGGGAGAACGGCGCTTTGTGGGGCGGCGAGTGCC
TGCGGGTGAACTATCGCGAATGTGAGCACCTGGGCGGCTTGCCTGCAGTGGCGCTTCCGGGTGGCGATTTGGCGGCGAAG
CAGCCGTGGCGTAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAGTGGCAGAATTACCCTGAAACGGCAAGTGTGCA
ACAGCAAAACTGGAGCGTACTGGCGCGGGCCATTGAGCGTGGAATTAACGCGCCGCTGGCGTCATCGTGTGGGCGTTTGT
TCGATGCTGTGGCGGCGGCACTGGGCTGTGCGCCAGCCACGTTAAGTTATGAAGGTGAAGCGGCTTGTGCTCTGGAGGCG
CTAGCAGCCTCATGCGACGGAGTGACGCATCCGGTGACGATGCCGCGGGTGGACAATCAACTGGATCTCGCCACTTTCTG
GCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGCGTTTCATGATGCGCTGGCGCAGGGTTTTGCCG
CGTTGATGCGTGAGCAGGCCACGATGCGTGGTATCACTACGCTGGTATTTAGCGGCGGGGTTATTCATAACCGTTTGCTG
CGTGCACGTCTGGCGCATTATCTCGCTGATTTCACATTGCTGTTTCCACAGAGTTTACCGGCGGGTGATGGCGGTTTGTC
TCTGGGGCAGGGGGTTATTGCTGCGGCGCGTTGGTTAGCGGGTGAAGTCCAGAACGGATAA

Upstream 100 bases:

>100_bases
CTTACAGAACTGTTCTGGCATCAAAGGAATTCCCGGAAATATTCGTAGAATACGGAGCGGGCGGTATCGCCGACCTGTGA
CTGCCGGATGCGACGCTGTC

Downstream 100 bases:

>100_bases
AACGTGTTGCATTTGGACACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACACATGCGTCCCCGTAGGTCGGA
TAAGACGCGCCAGCGTCGCA

Product: carbamoyltransferase HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 766; Mature: 765

Protein sequence:

>766_residues
MSGWTGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQH
CPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAM
PYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPAN
PLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALAL
PPGFKNVPPVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWAS
EMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAK
QPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
RARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG

Sequences:

>Translated_766_residues
MSGWTGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQH
CPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAM
PYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPAN
PLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALAL
PPGFKNVPPVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWAS
EMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAK
QPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
RARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG
>Mature_765_residues
SGWTGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHC
PPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMP
YDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFH
LACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPANP
LQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALP
PGFKNVPPVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWASE
MNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQ
PWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEAL
AASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLR
ARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=750, Percent_Identity=97.8666666666667, Blast_Score=1414, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83496; Mature: 83365

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGWTGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
CCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVP
CEEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECC
DAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDK
CCHHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
EYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HHCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVP
EEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCHHHH
ELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGI
HHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
ADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNT
HHCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
FCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWAS
EEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECC
EMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHH
CEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIER
HHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPRVDNQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
LDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGWTGYAIHDGGNNSMAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
CCCEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVP
CEEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECC
DAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDK
CCHHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
EYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HHCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVP
EEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCHHHH
ELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGI
HHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
ADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNT
HHCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
FCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWAS
EEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECC
EMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHH
CEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIER
HHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPRVDNQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
LDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]