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The map label for this gene is hydN

Identifier: 209397141

GI number: 209397141

Start: 3665742

End: 3666269

Strand: Reverse

Name: hydN

Synonym: ECH74115_3963

Alternate gene names: 209397141

Gene position: 3666269-3665742 (Counterclockwise)

Preceding gene: 209397110

Following gene: 209397726

Centisome position: 65.8

GC content: 52.65

Gene sequence:

>528_bases
ATGAACCGTTTCATCATTGCTGACGCGAGTAAATGTATTGGTTGCCGTACCTGTGAAGTGGCGTGCGTGGTTTCTCATCA
GGAAAATCAGGACTGTGCATCGCTGACCCCGGAAACATTTTTACCGCGTATCCATGTCATTAAAGGTGTGAACATTTCCA
CGGCGACAGTTTGCCGTCAGTGTGAAGATGCACCGTGCGCTAACGTCTGCCCGAATGGTGCTATCAGCCGTGATAAAGGG
TTTGTTCATGTGATGCAGGAACGTTGCATTGGTTGCAAAACCTGCGTTGTGGCTTGCCCGTATGGCGCGATGGAAGTGGT
GGTACGTCCGGTGATTCGCAACAGCGGCGCAGGGCTGAATGTACGGGCTGACAAAGCCGAAGCCAATAAATGCGACCTGT
GCAACCATCGTGAAGACGGCCCGGCGTGTATGGCGGCTTGCCCGACCCATGCGCTAATTTGTGTCGATCGCAATAAACTT
GAACAACTGAGCGCAGAAAAACGCCGCCGTACGGCGCTGATGTTCTAA

Upstream 100 bases:

>100_bases
CCTGCATTACAGAAATTAATTTCCTTAAATAACAGCAAATTAAAAACTGGCATGATTTGTGAATGTATCGGCGCATTAAC
TGTCATTGCTGGAGAATTTG

Downstream 100 bases:

>100_bases
AAATCTTACAGAACTGTTCTGGCATCAAAGGAATTCCCGGAAATATTCGTAGAATACGGAGCGGGCGGTATCGCCGACCT
GTGACTGCCGGATGCGACGC

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Sequences:

>Translated_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF
>Mature_175_residues
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKG
FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
EQLSAEKRRRTALMF

Specific function: Electron transport from formate to hydrogen

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=100, Blast_Score=355, Evalue=1e-100,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=61.7283950617284, Blast_Score=194, Evalue=2e-51,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=48.8372093023256, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=40.1197604790419, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=39.3063583815029, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1789079, Length=190, Percent_Identity=39.4736842105263, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI87082114, Length=199, Percent_Identity=34.6733668341709, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.7683615819209, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=33.1325301204819, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI226510944, Length=158, Percent_Identity=35.4430379746835, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=154, Percent_Identity=33.7662337662338, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1787963, Length=174, Percent_Identity=28.1609195402299, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=197, Percent_Identity=28.9340101522843, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYDN_ECO57 (P0AAK6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91075
- RefSeq:   NP_289262.1
- RefSeq:   NP_311596.1
- ProteinModelPortal:   P0AAK6
- SMR:   P0AAK6
- EnsemblBacteria:   EBESCT00000027833
- EnsemblBacteria:   EBESCT00000058164
- GeneID:   914709
- GeneID:   960982
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4021
- KEGG:   ecs:ECs3569
- GeneTree:   EBGT00050000009106
- HOGENOM:   HBG726839
- OMA:   DSSKCIG
- ProtClustDB:   PRK10330
- BioCyc:   ECOL83334:ECS3569-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 19026; Mature: 19026

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
10.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRIHVIKGVNISTATVCRQ
CCEEEEECCCHHCCCCCEEEEEEECCCCCCCHHHCCHHHHCCEEEEEECCCCHHHHHHHH
CEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHCCCCCCE
VRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF
EECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796