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The map label for this gene is gatC

Identifier: 209397449

GI number: 209397449

Start: 2843259

End: 2844614

Strand: Reverse

Name: gatC

Synonym: ECH74115_3071

Alternate gene names: 209397449

Gene position: 2844614-2843259 (Counterclockwise)

Preceding gene: 209399554

Following gene: 209395932

Centisome position: 51.05

GC content: 51.18

Gene sequence:

>1356_bases
ATGTTTTCAGAAGTCATGCGTTATATTCTCGATCTCGGCCCTACGGTGATGTTGCCGATTGTCATCATTATTTTTTCTAA
AATTTTAGGCATGAAGGCAGGCGATTGCTTTAAAGCGGGTCTGCATATCGGGATTGGCTTTGTTGGCATTGGCCTTGTGA
TTGGCTTAATGCTGGATTCCATTGGCCCGGCGGCGAAAGCGATGGCGGAAAATTTCGACCTGAATCTGCATGTGGTCGAT
GTCGGCTGGCCGGGCTCTTCACCAATGACCTGGGCGTCGCAAATTGCGCTGGTGGCGATTCCGATTGCGATTCTGGTTAA
CGTGGCGATGTTACTGACCCGTATGACGCGGGTGGTAAATGTTGATATCTGGAATATCTGGCATATGACCTTCACCGGCG
CGTTGCTGCATCTGGCAACCGGTTCATGGATGATAGGGATGGCAGGTGTGGTAATTCACGCGGCGTTTGTTTATAAGCTC
GGCGACTGGTTTGCCCGCGATACCCGAAATTTCTTTGAGCTGGAAGGCATTGCTATTCCGCACGGTACGTCGGCGTATAT
GGGGCCGATTGCGGTGCTGGTCGATGCTATCATCGAGAAAATCCCAGGCGTTAACCGTATTAAATTTAGCGCCGACGATA
TTCAGCGCAAATTTGGCCCGTTTGGCGAGCCTGTCACCGTGGGTTTTGTGATGGGGCTGATTATCGGCATCCTCGCGGGT
TACGATGTCAAAGGTGTATTGCAGCTGGCGGTAAAAACGGCGGCGGTGATGCTGCTAATGCCACGGGTGATTAAACCCAT
CATGGATGGTTTAACGCCCATCGCTAAGCAGGCCCGTAGTCGTTTACAGGCGAAGTTCGGCGGTCAGGAGTTCCTGATTG
GCCTGGATCCCGCATTACTGCTGGGGCATACGGCGGTGGTATCGGCAAGCCTGATTTTTATTCCGCTCACCATTTTAATT
GCTGTTTGTGTTCCTGGTAATCAGGTACTGCCGTTTGGCGATCTTGCCACTATCGGCTTCTTCGTGGCGATGGCGGTCGC
GGTGCATCGTGGAAATCTGTTCCGCACCTTAATCTCGGGTGTCATCATTATGAGCATCACCCTGTGGATCGCGACGCAAA
CTATTGGTTTGCACACCCAACTGGCGGCTAATGCTGGGGCGTTAAAAGCCGGGGGTATGGTGGCTTCAATGGATCAGGGC
GGTTCTCCCATTACCTGGTTACTGATTCAGGTTTTCTCCCCGCAAAATATTCCCGGTTTCATTATTATCGGCGCAATTTA
TCTTACCGGTATTTTCATGACCTGGCGTAGAGCGCGTGGCTTTATTAAACAAGAGAAAGTCGTTCTCGCAGAATAA

Upstream 100 bases:

>100_bases
TTTTGGCGATATTCCGTTAGTTCACGGCATGCCTTTTGTTTCTGGTGTCGGTATCGAAGCATTACAAAATAAAATTCTGA
CTATCTTACAGGGGTGACCT

Downstream 100 bases:

>100_bases
TTTTTACCTGAGGGGGAGGTTATCCCCCTTAACAATTAGGAGTTTTTATGAAATCAGTGGTGAATGATACTGATGGTATC
GTGCGCGTTGCAGAAAGCGT

Product: PTS system, galactitol-specific IIC component

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE

Sequences:

>Translated_451_residues
MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE
>Mature_451_residues
MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1788408, Length=451, Percent_Identity=100, Blast_Score=892, Evalue=0.0,
Organism=Escherichia coli, GI1790758, Length=384, Percent_Identity=45.5729166666667, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTKC_ECO57 (P69832)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85836
- PIR:   G90990
- RefSeq:   NP_288594.1
- RefSeq:   NP_310922.1
- ProteinModelPortal:   P69832
- EnsemblBacteria:   EBESCT00000027257
- EnsemblBacteria:   EBESCT00000057990
- GeneID:   916597
- GeneID:   959443
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3255
- KEGG:   ecs:ECs2895
- GeneTree:   EBGT00050000011294
- HOGENOM:   HBG470334
- OMA:   NIPGFIV
- ProtClustDB:   CLSK872636
- BioCyc:   ECOL83334:ECS2895-MONOMER
- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703
- TIGRFAMs:   TIGR00827

Pfam domain/function: PF03611 EIIC-GAT

EC number: NA

Molecular weight: Translated: 48366; Mature: 48366

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS51104 PTS_EIIC_TYPE_2; PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21a97550)-; HASH(0x223a9ab8)-; HASH(0x2208e418)-; HASH(0x21792378)-; HASH(0x223411c8)-; HASH(0x224b9f74)-; HASH(0x22278e90)-; HASH(0x2151ef0c)-; HASH(0x223dd45c)-; HASH(0x223434b4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDS
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVN
CCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIP
EEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECC
HGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
YDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
LGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLIQVFSPQNIPGF
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHEEHCCCCCCCHH
IIIGAIYLTGIFMTWRRARGFIKQEKVVLAE
HHHHHHHHHHHHHHHHHHHCCHHHCCEEECH
>Mature Secondary Structure
MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDS
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVN
CCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIP
EEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECC
HGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
YDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
LGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLIQVFSPQNIPGF
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHEEHCCCCCCCHH
IIIGAIYLTGIFMTWRRARGFIKQEKVVLAE
HHHHHHHHHHHHHHHHHHHCCHHHCCEEECH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796